Appendix - Examples; Example: Single Test Run - Agilent Technologies 7100 Technical Note

APPENDIX - Examples

Example: Single test run

For testing the functions of the 7100 CE and Empower 3 a test method can
be created. The equipment and method parameters can be summarized as
follows:
Capillary:
Electrolyte:
Home vials:
Sample:
The sample has to be diluted 1:10 before the run.
NOTE
Voltage:
Temperature:
Stop Time:
Preconditioning:
Injection:
Post-injection:
DAD:
DAD Timetable:
The run can be executed as single run (in this case save the instrument
method as well as the method set). If defined in the method, the online plot
shows the selected signals and the final result electropherogram can be
obtained in the Browse menu of Empower or via the projects.
Ensure that the Run Time is the same as defined in the CE/DAD method.
NOTE
Agilent 7100 CE in Empower Environment
Fused silica capillary 50 µm ID, 48.5 cm total (40 cm effective),
ext. Light path (G1600-60232)
20 mM Borate (from IQ-Kit: 5063-6514)
1 and 2 (filled with electrolyte solution)
4-Hydroxyacetophenone solution 1 mmol/l (from IQ-Kit: 5063-6514)
+30 kV
25°C
4 min
300 s (from electrolyte vial 3 into waste vial)
50 mbar for 10 s (from injection vial to outlet vial)
50 mbar for 5 s (from inlet vial to outlet vial)
Signal: 200 nm (BW 5 nm)
Reference: 360 nm (BW 100 nm)
Peakwidth: >0.025 min (10 Hz)
0.5 min Balance
APPENDIX - Examples
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