Surfactants, Anionic (Also Called: Detergents) Crystal Violet Method - Hach DR/850 Procedures Manual

SURFACTANTS, ANIONIC
(Also called: Detergents) Crystal Violet Method
1.
Enter the stored
program number for
Surfactants, anionic
(LAS).
Press:
PRGM
The display will show:
PRGM ?
5.
Add the contents of
one Detergents Reagent
Powder Pillow to the
funnel. Stopper the
funnel and shake to
dissolve the powder.
* Analytical Chemistry, 38, 791(1966).
(0 to 0.300 mg/L)
2.
Press:
26 ENTER
The display will show
mg/L, LAS and the
ZERO icon.
6.
Add 30 mL of
benzene to the funnel.
Stopper the funnel and
shake gently for
one minute.
Note: Spilled reagent will
affect test accuracy and is
hazardous to the skin and
other materials.
Note: Use benzene only in
a well-ventilated area.
For water, wastewater, and seawater
*
3.
Fill a clean 500-mL
graduated cylinder to the
300-mL mark with
sample. Pour the sample
into a clean 500-mL
separatory funnel.
7.
Place the separatory
funnel in a support stand.
417
Method 8028
4.
Add 10 mL of
Sulfate Buffer Solution.
Stopper the funnel.
Shake the funnel for
five seconds.
8.
Press:
TIMER ENTER
A 30-minute reaction
period will begin.
Note: Excessive agitation
may cause an emulsion,
requiring a longer time for
phase separation. If this
occurs, remove most of the
water layer, then gently
agitate the funnel with a
clean inert object in the
funnel such as a Teflon-
coated magnetic
stirring bar.