Creating And Editing Protocols: Advanced Options; Temperature And Dwell Time Options (F3-Option) - BIO RAD MyCycler Instruction Manual

The Clear key may be used to remove all numbers in the cycle repeat field.
5.4 Creating and Editing Protocols: Advanced Options
When creating or editing a protocol, the system provides additional functionality
in the use of the following:
• Temperature, Dwell Time, Temperature Gradient Options (F3-Option)
• Changing the Number of Cycles or Steps (F4-Add/Del)
5.4.1 Temperature and Dwell Time Options (F3-Option)
To add periodic increments or decrements to set point temperatures or dwell
times, put the cursor on the temperature or dwell time that you want to affect and
press F3-Option.
If the cursor is on a temperature field when the you press F3-Option, a menu
will offer the choices Constant Temp, Increment Temp, and Decrement
Temp, Temp Gradient, Max Ramp Rate and Set Ramp Rate (Figure XX).
• If the cursor is on a dwell time field when you press F3-Option, the choices in
the menu are Constant Time, Increment Time, and Decrement Time.
• If you press F3 when the cursor is somewhere other than a temperature or
dwell time field, a message will be displayed advising that no options are
available for this item. /->
Increment/Decrement Temperature: When you select one of these options, a
field will be displayed immediately above the set point temperature field. For
temperature increments, a thermometer icon and an up arrow will appear next to
the increment field. For temperature decrements, a thermometer icon and a down
arrow will appear next to the decrement field. The specified value will be added to
or subtracted from the set point temperature accordingly, for each repeat after
repeat one.
Use the number keys to input a temperature increment or decrement value.
You may set the increment or decrement as low as 0.1 °C, and as large as you like,
as long as the combination of the increment or decrement and the number of
repeats does not result in a temperature outside the range of the MyCycler
(4.0-1 oo.rrc).
Increment/Decrement Time: When you select one of these options, a field will be
displayed immediately below the dwell time field. For time increments, a clock icon
and an up arrow will appear next to the increment field. For time decrements, a
clock icon and a down arrow will appear next to the decrement field. The specified
value will be added to or subtracted from the dwell time accordingly, for each
repeat after repeat one.
Use the number keys to input a time increment or decrement value. You may
set the increment or decrement as low as 1.0 seconds, and as long you like, as _^
long as the combination of the increment or decrement and the number of repeats
does not result in a dwell time outside the range of the MyCycler (00:00-99:59).
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