Thermal Cycling Protocols; Overview; The Protocol Library Screen; My Protocols - BIO RAD MyCycler Instruction Manual

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Section 4
Thermal Cycling Protocols
4.1 Overview
Thermal cycling protocols contain the temperature cycling information to direct
the MyCycler. A protocol is made up of as many as nine cycles, and a cycle is
made up of as many as nine steps. A step is defined by specifying the set point
temperature and dwell time at that temperature. A cycle is defined by specifying
the times and temperatures for all steps and the number of times the cycle is
repeated. The MyCycler comes with a set of predefined Bio-Rad protocols in the
Protocol Library. You may also create new protocols by modifying any existing
protocol in the library, including the Bio-Rad protocols, or you can create a new
protocol from scratch.
4.2 The Protocol Library Screen
The Protocol Library screen contains the list of all thermal protocols saved on
the instrument. All user-defined protocols appear on the left-hand side of the
screen in the column labeled "My Protocols". Bio-Rad template protocols appear
on the right-hand side of the screen in the column labeled "Bio-Rad". Use the
arrow keys to select a protocol and then press Enter for more options. (See
Section 4.2.3 Protocol Options)
t*
Usei-defined Protocols ■
Use arrow keys to select a protocol,
and then press Enter.
i My Protocols
My First Protocol
Bio-Rad |
WIIHSIITilHinU
RT-PCR-2
LONG-PCR-2
CVCLE-SEQ2
NESTED PRIM2
TOUCHDOWN
STflNDfiRD-3
RT-PCR-3
LONG-PCR-3
CYCLE-SEQ3
Bio-Rad Template
Protocols
4.2.1 My Protocols
User-defined protocols are displayed in the Protocol Library under the column
labeled "My Protocols". The MyCycler has sufficient memory to store up to 99
total protocols in the library. You may run, view, edit, delete, or rename any of
these protocols listed in the My Protocols column.
4.2.2 Bio-Rad Protocols
Bio-Rad protocols are displayed in the Protocol Library under the column
labeled "Bio-Rad". These protocols serve as templates for several different PCR
applications. These include standard PCR, reverse transcriptase PCR (RT-PCR),
nested primer PCR, PCR with temperature gradients, long PCR, touchdown PCR,
and cycle sequencing PCR. These Bio-Rad protocols may be run directly, or
modified as needed prior to running or saving. If you wish to modify a Bio-Rad
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