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Beckman Coulter Manuals
Laboratory Equipment
J6 series
Instructions for use manual
Beckman Coulter J6 series Instructions For Use Manual
Rotors and tubes
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Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
Revision History
Safety Notice
Alerts for Warning, Caution, Important, and Note
Chemical and Biological Safety
Mechanical Safety
Rohs Notice
Table of Contents
Rotors
Introduction
General Description
Rotor Designations
Fixed-Angle, Swinging-Bucket, Vertical-Tube, and Rack-Type Rotors
Materials
Drive Pins
Rotor Selection
Rotors Used in Beckman Coulter J Series Centrifuges
Particle Separation in Fixed-Angle, Swinging-Bucket, and Verti
Pelleting (Differential Separation)
Sedimentation Coefficients (in Svedberg Units) for some Com
Nomogram for J2 Series Centrifuges
Nomogram for J6Series Centrifuges
Isopycnic Separations
Rate Zonal Separations
Blood Component Separations
General Operating Information
Rotor Balance
Bucket Rotors
Rotor Tie-Down
Arranging Tubes Symmetrically in a Fixed-Angle, Vertical-Tube, or JS-24 Series Swinging-Bucket Rotor
Overspeed Protection
Allowable Run Speeds
Temperature Compensation
Avanti J Centrifuges
Avanti J2 Series Centrifuges (no Longer Manufactured)
Analog J6 Series Centrifuges (no Longer Manufactured)
Microprocessor-Controlled Centrifuges
Tubes, Bottles, and Accessories
Introduction
Labware Selection Criteria
Labware Material Compatibility with Solvents and Sample
Characteristics and Chemical Resistances of Tube and Bottle
Gradient Formation and Fractionation
Labware Types
Polypropylene Tubes
Open-Top Polypropylene Tubes
Quick-Seal Polypropylene Tubes
Polycarbonate Tubes
Polyethylene Tubes
Ultra-Clear Tubes
Stainless Steel Tubes
Microfuge Tubes
Bottles
Multiwell Titer Plates
96-Well Microtiter Plates
Deep-Well Titer Plates (and Caps)
Square-Well Titer Plates
Temperature Limits
Spacers and Floating Spacers
Adapters
Bottle Adapters
Multitube Adapters
Solid Multitube Adapters
Modular Disk Adapters
Bottle and Tube Caps
Aerosolve Canisters
Blood Bag Cups
Rotor Labware Assemblies
Tubes, Bottles, and Accessories
Introduction
Gradient Preparation
General Filling and Sealing or Capping Requirements
General Filling and Sealing Requirements for Tubes and
Working with Physiological Fluids
Filling Open-Top Tubes
Open-Top Polypropylene Tubes
Swinging-Bucket Rotors
Fixed-Angle Rotors
Other Open-Top Tubes
Polycarbonate
Ultra-Clear
Polypropylene
Polyethylene
Stainless Steel
Capping Tubes
Filling and Capping Tubes
Three-Piece Assemblies
JLA-8.1000 and JLA-9.1000 Bottle Cap/Closure
Filling and Loading Cups in the
Rotor
Filling and Sealing Quick-Seal Tubes
The Cordless Quick-Seal Tube Topper
Capping Multiwell Titer Plates
Cap Strips
Aluminum Foil Lids
Using Adapters
Using Solid Multitube Adapters
Using Modular Disk Multitube Adapters
Using Aerosolve Canisters
Using Canisters as Wide-Mouth Bottles
Using Canisters with Tube Racks
Aerosolve Tube Rack
Using Blood Bag Cups
Sample Recovery
Capped Tubes
Cups
Quick-Seal Tubes
Making Ultra-Clear Tubes Wettable
Using Fixed-Angle Rotors
Introduction
Description
Examples of Fixed-Angle Rotors
General Specifications for Beckman Coulter J Series Fixed-Angle Rotors
Tubes and Bottles
Rotor Preparation and Loading
Prerun Safety Check
Rotor Preparation
Operation
Installing the Rotor
Removal and Sample Recovery
Using Swinging-Bucket Rotors
Introduction
Description
Examples of Swinging-Bucket Rotors
General Specifications for Beckman Coulter J Series Swinging
Labware
Rotor Preparation and Loading
Prerun Safety Check
Rotor Preparation
Special Preparation Instructions for JS-24 Series Rotors
Loading the Rotor Yoke
Loading JS-24 Series Rotors
Symmetric and Balanced Loading
Examples of Correctly and Incorrectly Loaded Buckets and Carriers
Loading Buckets
Using Modular Disk Adapters
Using Blood Bag Cups
Loading Buckets into the Rotor
Typical Blood Bag Loading Procedures
Using Microtiter Plate Carriers
Micro Plus Carriers
JS-5.9 and JS-5.3 Plate Carriers
The Micro Plus Microtiter Plate Carrier, Base, Pad, and Deep
J6 Carriers
Microplate Carriers Used with J6-Series Rotors
Operation
Sample Recovery
Removing JS-24 Series Rotors
And Rack-Type Rotors,6-1
Using Vertical-Tube and Rack-Type Rotors
Introduction
Description
General Specifications for Beckman Coulter J Series Vertical-Tube and Rack-Type Rotors
Vertical-Tube Rotors
Rack-Type Rotors
Vertical-Tube Rotor
Rack-Type Rotor
Using a Vertical-Tube Rotor
Tubes and Bottles
Rotor Preparation and Loading
Prerun Safety Checks
Rotor Preparation
Operation
Installing the Rotor
Removal and Sample Recovery
Using a Rack-Type Rotor
Trays and Tubes
Rotor Preparation and Loading
Prerun Safety Checks
Rotor Preparation
Operation
Installing the Rotor
Removal and Sample Recovery
Care and Maintenance
Introduction
Rotor Care
Cleaning
Decontamination
Sterilization and Disinfection
Inspection
Lubrication
O-Rings
Pivot Pins and Buckets
Field Rotor Inspection Program
Tube, Bottle, and Accessory Care
Cleaning
Decontamination
Sterilization and Disinfection
Tube and Bottle Sterilization and Disinfection
Inspection
Tube and Bottle Storage
Returning a Rotor or Accessory to the Factory
Diagnostic Hints
Troubleshooting Chart
Products,A-1
Chemical Resistances for Beckman Coulter Centrifugation Products
List of Chemical Resistances
Temperature Compensation Tables
Introduction
Temperature Compensation
Temperature Compensation Settings for Thej2-HC Centrifuge
Temperature Compensation Settings for the J2-21, J2-21B
Temperature Compensation Settings for the J2-MI, J2-21M, J2-MC, and J2-21M/E Centrifuges
Temperature Compensation Settings for the J6
Gradient Materials
Introduction
Materials
Commonly Used Gradient Materials with Their Solvents
Density, Refractive Index, and Concentration Data-Cesium
Density, Refractive Index, and Concentration Data-Sucrose at
Blood Component Separation
Introduction
Blood Bank Collection Overview
Components and Typical Usage
Single-Donor Fresh Plasma
Single-Donor Plasma
Packed Red Blood Cells
Leukocyte-Depleted Red Blood Cells
Shelf Life
Freezing
Separation of Blood Components by Centrifugation
Blood Component Storage
Blood Component Separation
Blood Bank Methods
Tips for Optimum Centrifugation Runs
References
List of References
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Rotors
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General Description
3
Troubleshooting Chart
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PN JR-IM-10AG
October 2014
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Instructions For Use
Rotors and Tubes
For Beckman Coulter
J2, J6, and Avanti J Series Centrifuges
Table of
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Summarization of Contents
Revision History
Revision AE, December 2013
Details changes made in Revision AE, dated December 2013.
Revision AF, February 2014
Details changes made in Revision AF, dated February 2014.
Revision AG, October 2014
Details changes made in Revision AG, dated October 2014.
Safety Notice
Alerts for Warning, Caution, Important, and Note
Explains the meaning of warning symbols and terms used in the manual.
Chemical and Biological Safety
Covers safety precautions for handling hazardous materials.
Mechanical Safety
Discusses safety measures related to mechanical operation and maintenance.
RoHS Notice
China RoHS Caution Label
Explains the meaning of the China RoHS caution label.
China RoHS Environmental Label
Explains the meaning of the China RoHS environmental label.
Scope of this Manual
Chapter 1: Rotors Overview
Outlines the content of Chapter 1 on rotors.
Chapter 2: Labware Overview
Outlines the content of Chapter 2 on labware.
Chapter 3: Filling, Sealing, and Recovery
Outlines the content of Chapter 3 on labware handling.
CHAPTER 1 Rotors
Introduction to Rotors
Introduces the chapter on J series rotors.
Rotor General Description
Provides an overview of rotor types and design.
Rotor Selection and Use
Guides users on selecting and using rotors.
CHAPTER 2 Tubes, Bottles, and Accessories
Labware Selection Criteria
Lists factors for choosing appropriate labware.
Labware Types Overview
Describes various types of labware available.
Temperature Limits for Labware
Discusses temperature limitations for various plastic labware.
CHAPTER 3 Filling, Sealing, and Sample Recovery
Gradient Preparation Methods
Explains methods for preparing density gradients.
General Filling and Sealing Requirements
Outlines general requirements for filling and sealing tubes.
Working with Physiological Fluids
Provides safety guidelines for handling physiological fluids.
CHAPTER 4 Using Fixed-Angle Rotors
Introduction and Description
Introduces and describes fixed-angle rotors.
Rotor Preparation and Loading
Details steps for preparing and loading fixed-angle rotors.
Operation and Installation
Provides operational guidance and installation steps.
CHAPTER 5 Using Swinging-Bucket Rotors
Introduction and Description
Introduces and describes swinging-bucket rotors.
Rotor Preparation, Loading, and Balancing
Details preparation, loading, and balancing for swinging-bucket rotors.
Bucket and Carrier Loading Procedures
Instructions for loading buckets, carriers, and specific adapters.
CHAPTER 6 Using Vertical-Tube and Rack-Type Rotors
Introduction and Description
Introduces and describes vertical-tube and rack-type rotors.
Vertical-Tube Rotor Operation
Instructions for using vertical-tube rotors.
Rack-Type Rotor Operation
Instructions for using rack-type rotors.
CHAPTER 7 Care and Maintenance
Rotor Care Fundamentals
Outlines essential aspects of proper rotor care.
Cleaning, Decontamination, and Sterilization
Details procedures for cleaning, decontaminating, and sterilizing rotors.
Inspection and Lubrication
Emphasizes inspection and lubrication for rotor maintenance.
APPENDIX B Temperature Compensation Tables
Introduction to Compensation Tables
Introduces the appendix on temperature compensation.
Temperature Compensation Settings
Lists temperature compensation units for various rotors.
APPENDIX D Blood Component Separation
Blood Bank Collection Overview
Describes the process of collecting and processing whole blood.
Blood Component Usage and Storage
Details blood components, their uses, and storage life.
Centrifugation for Blood Separation
Explains centrifugation methods for blood component separation.
APPENDIX E References
List of Available Documents
Lists documents for obtaining further information.
Glossary
Glossary of Terms
Defines key terms used in the manual.
Table of Contents
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