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POLYACRYLIC ACID,
Lever
13.
Rinse the
syringe by shaking.
Discard the eluant
through the tubing.
Note: Move the plunger
up and down several
times to clear the
tubing.
Lever
16.
Rotate the valve
lever to the pump
(left) position. Over a
period of 30 seconds
force the eluant
through the LC
cartridge. Collect the
eluant in a 25-mL
tall-form graduated
cylinder.
continued
Lever
14.
Draw at least 10
cc of eluant into the
syringe through the
tubing followed by
air past the 25-cc
mark.
17.
Fill the cylinder
to exactly 25 mL with
demineralized water.
Stopper and invert to
mix (the blank).
Note: Up to five
samples can be run with
one blank.
Note: Volumes are
critical at this point.
The cylinders can be
matched by pipetting
25.00 mL of
demineralized water
into each, and marking
them at the correct
volume. Tall-form
cylinders must be used.
50-59
Lever
15.
Push the plunger
down to adjust the
eluant volume to
exactly the 10-cc
mark.
Lever
18.
Clean the
syringe and cartridge
by drawing in 25 cc
of demineralized
water in the aspirate
(down) position.
Discard the water
back through the
tubing.
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