Download Print this page

Hach DR/700 Procedures Manual

Hach DR/700 Procedures Manual

Colorimeter

Table Of Contents

page of 762

/ 762
Contents
Table of Contents
Bookmarks

Table of Contents

Advertisement

Quick Links

46014-88

DR/700

COLORIMETER

PROCEDURES MANUAL

©Hach Company, 1990-2001. All rights reserved. Printed in U.S.A.

7-30-96-9ed

Rev. 2b, 11/01

Table of Contents

Advertisement

Table of Contents

Need help?

Need help?

Do you have a question about the DR/700 and is the answer not in the manual?

Questions and answers

Summary of Contents for Hach DR/700

Page 1 46014-88 DR/700 COLORIMETER PROCEDURES MANUAL ©Hach Company, 1990-2001. All rights reserved. Printed in U.S.A. 7-30-96-9ed Rev. 2b, 11/01...
Page 3: Table Of Contents
Introduction Trademarks of Hach Company ....... .v Sample Procedure Explained ....... vii Section I Chemical Analysis Information Abbreviations and Conversions.
Page 4 Section II Procedures DR/700 Filter Module Selection Guide ..... . . 2-1 Procedure Listing by Parameter (alphabetical) ....2-1 Procedure Listing by Filter Module .
Page 5: Trademarks Of Hach Company
Additional information on the chemical reactions of many of the procedures is contained in the Hach Water Analysis Handbook, Publication 8353, available free on request.
Page 7: Sample Procedure Explained
(Powder Pillows or AccuVac Ampuls), used USEPA accepted for reporting** USING POWDER PILLOWS Acceptance of method by USEPA if applicable Install DR/700 Press: After 2 seconds, module the display will show a 81.01 The display will show program number, in the instrument.
Page 8 Illustration of procedure steps and instrument keystrokes required PHOSPHORUS, REACTIVE, continued 2 minutes Fill a 10-mL cell to Add the contents of Wait two minutes. the 10-mL line with one PhosVer 3 sample. Phosphate Powder Note: An 8-10 minute Pillow to the sample reaction period should be cell (the prepared Note: For proof of...
Page 9 PHOSPHORUS, REACTIVE, continued Fill a 10-mL cell to Place the blank in Press: ZERO the 10-mL line with the cell holder. sample (the blank). The display will count Note: In bright down to 0 Then the display will show 0.00 sunlight it may be mg/l, and the zero necessary to close the...
Page 10 PHOSPHORUS, REACTIVE, continued USING ACCUVAC AMPULS Alternate method Install DR/700 Press: After 2 seconds, module the display will show a 81.01 The display will show program number, in the instrument. 810 nm concentration units, and module number decimal position and 81.01...
Page 11 PHOSPHORUS, REACTIVE, continued 30 seconds Fill a cell with 10 Fill a PhosVer 3 6. Place an ampul cap mL of sample (the Phosphate AccuVac securely over the tip of blank). Cap. Collect at Ampul with sample. the ampul. Shake the least 40 mL of sample ampul for approximately in a 50-mL beaker.
Page 12 PHOSPHORUS, REACTIVE, continued Insert the Place the Press: READ AccuVac Vial Adapter prepared sample in the into the cell holder. cell holder. The display will count down to 0. Then the display will show the Note: In bright sunlight it may be necessary to close results in mg/L the cell compartment cover.
Page 13 PHOSPHORUS, REACTIVE, continued ACCURACY CHECK Confirm accuracy with Standard Additions Method these steps a) Snap the neck off a Phosphate Voluette Ampule Standard Solution, 50 mg/L PO b) Use the TenSette Pipet to add 0.1 mL, 0.2 mL and 0.3 mL of standard, respectively, to three 25-mL water sample.
Page 14 STATISTICAL EVALUATION repeatability A single operator repetitively tested samples of two laboratory prepared of the solutions, using one DR/700, matched sample cells and two representative procedure lots of testing reagents. Testing 1.60 mg/L PO concentration samples, the standard deviation was ±0.007 mg/L PO Testing zero concentration samples, the limit of detection was 0.019 mg/L...
Page 15 Indicator Paper, 1 to 11 pH... . . 5 rolls/pkg ..391-33 pH Meter, Hach One ....each ..43800-00 Pipet, 2 mL serological .
Page 17: Section I Chemical Analysis Information
SECTION I Chemical Analysis Information...
Page 19: Abbreviations And Conversions
Health Association (APHA), the American Water Works Association (AWWA), and the Water Pollution Control Federation (WPCF). Order from Hach requesting Cat. No. 22708-00 or from the Publication Office of the American Public Health Association. This book is the standard reference work for water analysis. Many procedures contained in this manual are based on Standard Methods.
Page 20 Conversions Conversion factors for many of the commonly used units of measure have been included to make the use of this manual more universal and to simplify calculations. Conversions are categorized by test. Nitrogen Nitrite (NO ) = Nitrogen (N) x 3.28 Nitrate (N0 ) = Nitrogen (N) x 4.42 Ammonia (NH...
Page 21 Oxygen, Dissolved The following table lists the mg/L dissolved oxygen in water at saturation for various temperatures and atmospheric pressures. The table was formulated in a laboratory using pure water; thus, the values given should be considered as only approximations when estimating the oxygen content of a particular body of surface water.
Page 22: Accuracy And Precision
ACCURACY AND PRECISION Accuracy is the nearness of a test result to the true value. Precision refers to the agreement of a set of replicate results or repeatability. Although good precision suggests good accuracy, precise results can be inaccurate. The following paragraphs describe techniques to improve accuracy and precision of analysis.
Page 23 A decision tree, such as the one in Figure 1, establishes a systematic method for identifying the problem. Request Hach Publication 7004 for additional information on standard additions.
Page 24 Branch A Suppose a single standard addition to the sample did not give the correct concentration increase. A possible cause could be interferences. Other causes could be defective reagents, an incorrect procedure, a defective instrument and apparatus or a defective standard used for standard additions.
Page 25 If the results of multiple standard additions gave the correct increments between additions, proceed to Branch C. If the results of multiple standard additions gave other than the correct increments between additions, proceed to Branch D. Branch C If interfering ions are present, the analysis may be incorrect. However, it may be possible, with multiple standard additions, to arrive at a close approximation of the correct result.
Page 26 Dirty pipets and graduated cylinders are sources of contamination and will not deliver the correct volumes. Hach's TenSette Pipet for dispensing Standards and standards sealed in Voluette Ampules are ideal for standard additions.
Page 27 graph as A and B show that the four data points do not lie on a straight line. Plot A illustrates an interference becoming progressively worse as the concentration of the standard increases. This type of interference is not common and may be caused by an error or malfunction of the procedure, reagents or instrument.
Page 28 The apparent interference in Example B could be the result of an error made in the standard addition. The analysis should be repeated. The two examples illustrate chemical interferences which most certainly mean the result of the first analysis of the water sample was incorrect. When this type of interference occurs, the analyst should attempt to analyze the sample with an alternate method which uses a different type of chemistry.
Page 29: Usepa Approved
Federal Register and compiled in the Code of Federal Regulations. USEPA Accepted Hach has developed analytical methods that are equivalent to USEPA- approved methods. Even though minor modifications may exist, the USEPA has reviewed and accepted certain methods for reporting purposes.
Page 30: Adapting Procedures
ADAPTING PROCEDURES ADAPTING HACH PROCEDURES FOR USE WITH OTHER PHOTOMETERS Hach test procedures can be used with other instrumentation if calibration curves are established to convert test results from % transmittance or absorbance to the concentration of the constituent being measured.
Page 31 instrument reading was 46%, the 40 line and the 6 column would be selected. The test result would be 0.78 mg/L iron (Fe). If in Step 3, absorbance values are measured, plot the results on linear- linear paper. Plot the absorbance value on the vertical scale and the concentration values on the horizontal scale.
Page 32: Adapting A Buret Titration For Use With A Digital Titrator
Table 3 Calibration Table %T UNITS Tens 2.30 2.21 2.12 2.04 1.97 1.90 1.83 1.77 1.72 1.66 1.61 1.56 1.51 1.47 1.43 1.39 1.35 1.31 1.27 1.24 1.20 1.17 1.14 1.11 1.08 1.04 1.02 Adapting a Buret Titration for Use With a Digital Titrator Adapt any standard titration procedure using a buret to the Digital Titrator by using the following procedure.
Page 33 b. If the number of digits required is less than 70, increase the sample size to increase precision. c. If the sample size is altered, adjust the amount of buffering or indicating reagents by the same proportion. 4. When using the Digital Titrator for your buret method, note the number of digits required for a sample titration.
Page 34: Interferences
Table 4. Titration Cartridges Description Cat. No. CDTA, 0.0800 M, HexaVer .......14402-01 CDTA, 0.800 M, HexaVer .
Page 35: Ph Interference
Interference Many of the procedures in this manual are pH dependent. Hach reagents contain built-in buffers to adjust the pH of the typical sample to the correct pH range. However, the reagent buffer capacity may not be sufficient for some unusual samples.
Page 36: Interference From Stray Light
8. Analyze the sample as before. Interference From Stray Light Typical indoor lighting permits the DR/700 to operate with the cell compartment cover open. In bright sunlight, it may be necessary to close the cell compartment cover. If a 25-mL cell is used in the procedure, transfer 10 mL of the solution to a 10-mL cell.
Page 37 solution to pass through. This is especially helpful when sample turbidity interferes with calorimetric analysis. Two general methods of filtration are gravity and vacuum. Gravity filtration uses the force of gravity to pull the sample though the filter paper. Vacuum filtration uses the pressure difference created by either an aspirator or vacuum pump plus the force of gravity to move the sample through the filter.
Page 38 REQUIRED APPARATUS Description Unit Cat. No. Filter Discs, glass 47 mm ....100/pkg ..2530-00 Filter Holder, membrane ....each ... 2340-00 Flask, filter, 1000 mL .
Page 39: Reagent And Standard Stability
Chemicals supplied by Hach have an indefinitely long shelf life when stored under average room conditions, unless designated otherwise. Notations on product labels specify any special storage conditions required.
Page 40: Safety
the test is performed. This is done by zeroing the instrument on deionized water and reagents. In most cases, the reagent blank is so small the instrument is zeroed on either an untreated portion of the original water sample or deionized water.
Page 41: Sample Cell Matching
Refer to the MSDS for safety data essential for day-to-day operations and safety training. Sample Cell Matching The sample cells provided with the DR/700 Colorimeter are not optically perfect. Glass imperfections can introduce an error in the true absorbance or percent transmittance measurement. In turn, the true Absorbance or % T error can result in reduced accuracy.
Page 42: Sample Dilution Techniques
accuracy and precision, sample cells should be matched prior to use. Refer to Matching Sample Cells in the instrument manual supplied with the colorimeter. Sample Dilution Techniques Ten and Twenty-five milliliter (mL) are the specified volumes for most colorimetric tests. However, in some tests, the color developed in the sample may be too intense to be measured.
Page 43 The concentration of the sample is equal to the diluted sample reading times the multiplication factor. An example: A 2.5 mL sample was diluted with 22.5 mL of deionized water. The result was 0.35 mg/L. What is the concentration of the sample? 0.35 x 10 = 3.5 mg/L More accurate dilutions can be done with a pipet and a 100-mL volumetric flask.
Page 44: Temperature Considerations
Temperature Considerations For best results, most tests described in this manual should be performed with sample temperatures between 20 °C (68 °F) and 25 °C (77 °F). If certain tests require closer temperature control, that requirement will be indicated in notes following those procedures. Use of Pipets and Graduated Cylinders When small sample quantities are used, the accuracy of measurements is important.
Page 45: Use Of Accuvac Ampuls
Using AccuVac Ampuls Use of the DR/700 AccuVac Vial Adapter For safety and ease of use, the DR/700 AccuVac Vial Adapter is provided with the instrument for use with Hach Company's AccuVac Ampul Reagents. Insert the adapter in the cell compartment by aligning...
Page 46 it about 1/4 inch out from the slot in the front side of the cell holder as in Figure 8. Push the adapter against the slot sides to seat it. to be sure the adapter is seated, gently slide it upward. The adapter should stop about half way up and stay there.
Page 47: Use Of Reagent Powder Pillows
Use of Reagent Powder Pillows Dry powdered reagents are used when possible to minimize problems of leakage and deterioration. Powders are packaged in individual, pre- measured, polyethylene "powder pillows." Each pillow contains enough reagent for one test and is opened easily with nail clippers or scissors; see Figure 10.
Page 48: Using The Tensette Pipet
For best results, always use a new tip for each pipetting operation. After being used several times, the pipet tip may retain some liquid, causing an error in delivery, Each pipet is supplied with 100 tips. Order Hach replacements, for best results.
Page 49 3. Press down on the turret cap with the thumb, using a smooth motion, until the turret reaches the stop. Immerse the tip about 5 mm (¼") below the surface of the solution to avoid drawing air into the tip. Do not insert the tip any deeper, or the delivery volume may be affected.
Page 50 6. Use the thumb and forefinger to twist the turret cap to the next higher position on the volume-setting ring to assure full blowout and quantitative transfer of the sample. The "F" position provides full blowout for the 1.0-mL setting. 7.
Page 51: Mixing Water Samples
Mixing Water Samples The following two methods may be helpful in tests that require mixing sample with chemicals (usually indicated by "swirl to mix" instructions). 1. A swirling motion is recommended when mixing sample in a graduated cylinder or a titration flask. In this case, grip the cylinder (or flask) firmly with the tips of three fingers;...
Page 52: Volume Measurement Accuracy
Figure 13 Swirling Square Mixing Bottle Volume Measurement Accuracy Sample cells supplied with the colorimeter are marked to indicate approximately 10 mL or 10, 20, and 25 mL. In most tests where volume measurements are critical, the procedure specifies the appropriate method. If a sample must be diluted, use a pipet for volume measurement.
Page 53: Sample Pretreatment
This section has three different digestion procedures. The Hach Digesdahl system is an absolute process that yields a digest suitable for the determination of metals, total phosphorus and total kjeldahl nitrogen (TKN).
Page 54: Epa Vigorous Digestion With Hot Plate
6. Quantitatively transfer the sample with deionized water to a 100-mL volumetric flask and dilute to volume with deionized water. Continue with the procedure. This mild digestion may not suffice for all sample types. A reagent blank also should be carried through the digestion and measurement procedures.
Page 55 result by the correction factor in column 5 of the table below. A reagent blank also should be carried through the digestion and measurement procedures. Table 7. Vigorous Digestion Expected Suggested Suggested Suggested Correction Metal Sample for Volume Final Volume Factor Concentration Digestion 1:1 HC1...
Page 56: General Digesdahl Digestion Procedure
Volume % solids manuals for a variety of (mL) sample types are Note: Use only Hach available, free of charge digestion flasks. Example: If solids are from Hach Company. Volumetric flasks with 10% of total volume of...
Page 57 GENERAL DIGESDAHL DIGESTION, continued 3 - 5 minutes Place the flask Heat 3-5 minutes. Be sure you have weight followed by the Do not boil sample to added the correct fractionating column dryness. amount of sulfuric with funnel on the acid.
Page 58 GENERAL DIGESDAHL DIGESTION, continued Boil off excess Take the flask off hydrogen peroxide by the heater and allow heating for one more the flask to cool. minute after addition Remove the of hydrogen peroxide fractionating column is complete. Do not from the digestion heat to dryness.
Page 59 GENERAL DIGESDAHL DIGESTION, continued REQUIRED APPARATUS (continued) Quantity Description Per Digestion Unit Cat. No. Safety Glasses ... . . 1 ... each..18421-00 Safety Shield, for Digesdahl .
Page 60 Watch Glass, 65 mm ....12/pkg ..578-97 For additional ordering information, see final section. In the U.S.A. call 800-227-4224 to place an order. *Contact Hach for larger sizes. 1-42...
Page 61 Table 8. Digestion Guidelines for Specific Sample Types Sample Sample Amount Preheat Amount of Special Type Weight of Acid Time Peroxide Instructions Plant 0.25 to 5 g 4 mL 4 min. 10 mL Use N-free paper Tissue to weigh sample Meat &...
Page 62: Safety Considerations
•Sample size-Never exceed 0.5 grams of sample (dry weight) •Oils and organic liquids should be considered as solids when determining sample size. •Acid type- Only use acid specified in Hach step-by-step procedures. •Acid volume- Never use less than 3 mL. •Always follow the order of steps indicated.
Page 63 •Always wear safety glasses. •Always perform digestion behind a safety shield or in a closed fume hood. •If the sample goes to dryness, remove immediately and cool. Repeat procedure with smaller sample volume or more acid. The following additional specific safety precautions are appropriate when using hydrogen peroxide in the Digesdahl digestion applications: Do not mix hydrogen peroxide with any chemical reagents except as specified in the instructions.
Page 64 3. Hold the parts exposed to the digestion mixture under running water, avoiding getting water or the digestion mixture into the heater base. If the aspirator fails during a digestion, immediately turn the heater off. Do not breathe any fumes that may be expelled from the manifold. After the flask has cooled and the fumes subsided, clean or replace the aspirator.
Page 65 Do store in a cool place away from direct sunlight. Do store in the original containers with closures as supplied and keep closed when not in use. (Be sure the containers are vented. Hach hydrogen peroxide bottles are shipped with a special permeable cap liner.) Do wear gloves and safety glasses when handling the material.
Page 66: Application Specific Manuals
3201 Fluid Fertilizer Analysis Manual 8353 Water Analysis Handbook To receive a free copy of these manuals, contact Hach Customer Services and request by literature code number. DISTILLATION Distillation is an effective way of separating chemical components for analysis. The Hach Distillation Apparatus (see Figure 14) is adapted easily for a variety of test needs.
Page 67: Sampling And Storage
Figure 14 General Purpose Distillation Apparatus with Heater and Support Apparatus SAMPLING AND STORAGE Correct sampling and storage are critical to the accuracy of each test. For greatest accuracy minimize contamination from the sampling device, remove residues of previous samples from sample container and preserve the sample properly, if necessary.
Page 68: Acid Washing Bottles
taken near the center of the vessel or duct and below the surface. Use only clean containers (bottles, beakers) for collecting samples. Rinse the container several times first with the water to be sampled. Take samples as closely as possible to the source of the supply to minimize the effects of a distribution system.
Page 69: Storage And Preservation
glassware must be rinsed thoroughly with distilled water. For ammonia and Kjeldahl nitrogen, the glassware must be rinsed with ammonia-free water. Storage and Preservation The most cost-effective sample containers are made of polypropylene or polyethylene. The best and most expensive containers are made of quartz or TFE (tetrafluoroethylene, Teflon).
Page 70 Table 9. Required Containers, Preservation Techniques and Holding Times Maximum Parameter No./Name Container Preservation Holding Time Table 1A-Bacterial Tests: 1-4. Coliform, fecal Cool, 4°C, 0.008% 6 hours. and total 5. Fecal streptococci P, G Table 1B-Inorganic Tests: 1. Acidity P, G Cool, 4°C 14 days.
Page 71 Table 9 Required Containers, Preservation Techniques and Holding Times (continued) Maximum Parameter No./Name Container Preservation Holding Time Metals (continued): 35. Mercury P, G to pH < 2 28 days. 3, 5-8, 10, 12, 13, 19, 20, P, G 6 months. 22, 26, 29, 30, 32-34, 36, 37, 45, 47, 51, 52, 58-60, 62, 63, 70-72, 74, 75.
Page 72 Table 9 Required Containers, Preservation Techniques and Holding Times (continued) Maximum Parameter No./Name Container Preservation Holding Time Metals (continued): 61. Silica 28 days. 64. Specific conductance P, G 65. Sulfate P, G 66. Sulfide P, G Cool, 4 °C add 7 days.
Page 73: Volume Additions, Corrections For
Volume Additions, Correction For When significant amounts of preservative are used, a volume correction should be made. This will account for the acid added to preserve the sample and the base used to adjust the pH to the range of the procedure. This correction is made as follows: 1.
Page 74 1-56...
Page 75: Section Ii Procedures
SECTION II Procedures...
Page 77 DR/700 MODULE SELECTION GUIDE Parameters and Ranges (alphabetical) Module Number Reagent Description Range (Cat. No.) Package Aluminum, Aluminon Method ....0-1.0 mg/L ..52.01 (46252-00)..22420-00 Aluminum, Eriochrome Cyanine R Method .
Page 78 Module Number Reagent Description Range (Cat. No.) Package Nitrogen, Ammonia, Salicylate Method ..0-0-1.00 mg/L. . 61.01 (46261-00)..22437-00 Oxygen, Dissolved, High Range ... 0-10 mg/L ..52.01 (46252-00)..25150-25 Oxygen, Dissolved, Low Range.
Page 79 420-nm Filter Module (42.01) Cat. No. 46000-10 Parameter.........Range Benzotriazole, Tolytriazole.
Page 80 525-nm Filter Module (52.01) Cat. No. 46000-14 Parameter.........Range Aluminum, Aluminon .
Page 81 610-nm Filter Module (61.01) Cat. No. 46000-17 (continued) Parameter.........Range Oxygen Dissolved, LRDO AccuVac .
Page 83 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 84 Table of Contents for 420-nm parameters Benzotriazole, Tolyltriazole ....... 42-1 Copper, Porphyrin .
Page 85 For cooling and boiler water UV Photolysis Method* Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm the concentration Note: The most reliable and module units and the zero 42.01...
Page 86 BENZOTRIAZOLE AND TOLYLTRIAZOLE, continued Fill a square Add the contents Insert the mixing bottle with of one Triazole ultraviolet lamp into 25 mL of sample. Reagent Powder the mixing bottle. Pillow (the prepared Note: For proof of sample). Swirl to Note: UV safety goggles dissolve completely.
Page 87 BENZOTRIAZOLE AND TOLYLTRIAZOLE, continued 5 minutes Turn the UV lamp Wait 5 minutes. Turn the lamp off. Remove lamp from the bottle Swirl to Note: A yellow color mix thoroughly. will develop if triazole is Note: Low results will present. occur if photolysis (lamp on) takes place for more or less than...
Page 88 BENZOTRIAZOLE AND TOLYLTRIAZOLE, continued Press: ZERO Place the Press: READ prepared sample in The display will the cell holder. The display will count down to 0. count down to 0. Then the display will Note: In bright sunlight Then the display will show 0.00 mg/L and show the results in it may be necessary to...
Page 89 5.0 mg/L, replace the lamp. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 10.0 mg/L tolyltriazole concentration solutions, the standard deviation was ±0.22 mg/L tolyltriazole.
Page 90 Indicator Paper, 1 to 11 pH ... . . 5 rolls/pkg..391-33 Pipet Filler, safety bulb ....each..14651-00 *Contact Hach for larger sizes. 42-6...
Page 91 Timer, interval, 1 second to ten hours ..each..23480-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 42-7...
Page 92 42-8...
Page 93 For water, wastewater and seawater Porphyrin Method* Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm the concentration Note: If samples cannot and module number units and the zero 42.01...
Page 94 COPPER, continued Fill two 25-mL Add the contents Add the contents cells to the 25-mL of one Copper of one Porphyrin 1 line with sample. Masking Powder Reagent Powder Pillow to one of the Pillow to each sample Note: Wash all sample cells (the cell.
Page 95 Reagent Powder Pillow to each sample Note: Typical indoor cell. Cap and invert lighting permits the several times to mix. DR/700 to operate with the cell compartment Note: The yellow color open. In bright will turn blue sunlight, it may be momentarily.
Page 96 Then the display will µg/L and the zero show the results in lighting permits the prompt will turn off. µg/L copper DR/700 to operate with (Cu Porphyrin). the cell compartment open. In bright sunlight, Note: For most accurate it may be necessary to...
Page 97 Prepare this solution daily. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 150 µg/L Cu concentration solutions, the standard deviation was ±1.2 µg/L Cu.
Page 98 COPPER, continued Testing zero concentration samples, the limit of detection was 1.7 µg/L Cu. The limit of detection was calculated as three times the standard deviation when testing zero concentration samples (adapted from Analytical Chemistry, 1980, 52, 2242-2249). INTERFERENCES The following may interfere when present in concentrations exceeding those listed below: Aluminum 60 mg/L...
Page 99 ... 1 ..each..968-00 DR/700 Filter Module Number 42.01... . . 1 ..each..46242-00 OPTIONAL REAGENTS Copper Standard Solution, 10 mg/L Cu .
Page 100 Watch Glass......each..578-70 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information Outside the U.S.A. - Contact the Hach office or distributor serving you. 42-16...
Page 101 For water and wastewater Mercaptoacetic Acid Method* Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm the concentration Note: Collect samples and module number units, decimal 42.01...
Page 102 MOLYBDENUM, MOLYBDATE, HR, continued Fill a 25-mL cell Add the contents Add the contents to the 25-mL line of one MolyVer 1 of one MolyVer 2 with sample. Reagent Powder Reagent Powder Pillow. Cap and Pillow. Cap and invert Note: For proof of invert several times to several times to mix.
Page 103 0. Then the display will Note: Typical indoor lighting permits the show 0.00 mg/L and lighting permits the DR/700 to operate with the zero prompt will DR/700 to operate with the cell compartment turn off. the cell compartment cover open.
Page 104 , listed under Optional Reagents. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched cells and two representative lots of testing reagents. Testing 20.0 mg/L Mo concentration samples, the standard deviation was ±0.11 mg/L Mo.
Page 105 ... . 1 ..each..968-00 DR/700 Filter Module Number 42.01... . . 1 ..each..46242-00...
Page 106 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 42-22...
Page 107 Method 8037 NICKEL (0 to 1.80 mg/L Ni) For water, wastewater and seawater Heptoxime Method*; EPA accepted for reporting (Digestion is required; see Section 1)** 5 minutes Measure 300 mL Add the contents Wait 5 minutes. of sample in a 500-mL of one Nickel 1 graduate cylinder.
Page 108 Note: Point the funnel in a safe direction when venting. 5 minutes 30 seconds Close the Shake the funnel Install module stopcock. Shake for several times over the 42.01 30 seconds. next five minutes. in a DR/700. Vent after shaking. 42-24...
Page 109 NICKEL, continued Press: After 2 seconds, Wait for the the display will show layers to separate The display will show a program number, (about 5 minutes). 420 nm the concentration Insert a pea-size and module number units, decimal cotton plug into the 42.01 position and the zero delivery tube of the...
Page 110 Note: Typical indoor turn off. lighting permits the Note: The five-minute DR/700 to operate with reaction period is not the cell compartment necessary for the second cover open, In bright and third extractions. sunlight, it may be Shake with chloroform;...
Page 111 0. Note: Typical indoor Then the display will show the results in lighting permits the mg/L nickel (Ni). DR/700 to operate with the cell compartment cover open, In bright sunlight, it may be necessary to close the cell compartment cover.
Page 112 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.00 mg/L Ni concentration samples, the standard deviation was ±0.026 mg/L Ni.
Page 113 NICKEL, continued Testing zero concentration samples, the limit of detection was 0.013 mg/L Ni. The limit of detection was calculated as three times the standard deviation when testing zero concentration samples (Adapted from Analytical Chemistry, 1980, 52, 2242-2249). INTERFERENCES Cobalt, copper and iron interferences can be overcome by adding one or more additional Nickel 1 Reagent Powder Pillows in Step 2.
Page 114 Cylinder, graduated, 500 mL..1 ..each..508-49 DR/700 Filter Module Number 42.01... . . 1 ..each..46242-00 Funnel, separatory, 500 mL.
Page 115 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 42-31...
Page 116 42-32...
Page 117 Nessler Method**, EPA accepted for reporting (distillation required)† Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm the concentration Note: If samples cannot and module number units, decimal 42.01...
Page 118 NITROGEN, AMMONIA, continued Fill a 25-mL Fill another Add three drops sample cell to the 25-mL sample cell of Mineral Stabilizer 25-mL mark with with demineralized to each sample cell. sample (the prepared water (the blank). Cap and invert several sample).
Page 119 0. Then the display will show 0.00 Note: Typical indoor lighting permits the mg/L and the zero lighting permits the DR/700 to operate with prompt will turn off. DR/700 to operate with the cell compartment the cell compartment cover open. In bright cover open.
Page 120 Ammonium Nitrogen to 50.0 mL with demineralized water. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched cells and two representative lots of testing reagents. Testing 1.50 mg/L NH - concentration samples, the standard deviation was ±0.013 mg/L NH...
Page 121 9.5 with 1 N sodium hydroxide solution. Use a pH meter. c) Set up the general purpose distillation apparatus as shown in the Hach Distillation Apparatus Manual. Pour the solution into the distillation flask. Add a stir bar. d) Use a graduated cylinder to measure 25 mL of demineralized water into a 250-mL erlenmeyer flask.
Page 122 Water, demineralized ..25 mL ..4 L ... . . 272-56 REQUIRED APPARATUS DR/700 Filter Module Number 42.01... 1 ..each ..46242-00 Pipet, 1 mL .
Page 123 Thermometer, -20 to 105 °C ... . . each ... 1877-01 For Technical Assistance, Prices and Ordering In the U.S.A.--Call 800-227-4224 toll-free for more information. Outside the U.S.A.--Contact the Hach office or distributor serving you. *Contact Hach for larger sizes 42-39...
Page 124 42-40...
Page 125 Method 8000 OXYGEN DEMAND, CHEMICAL (COD) (0 to 150 mg/L) For water, wastewater and seawater Reactor Digestion Method*; USEPA approved for reporting† DIGESTION 2 minutes Remove the cap Homogenize 100 Turn on the COD from a 0 to 150 mg/L mL sample for 2 Reactor.
Page 126 OXYGEN DEMAND, CHEMICAL, continued Hold the vial at a Replace the vial Hold the vial by 45-degree angle. cap tightly. Rinse the the cap and over a Pipet 2.00 mL of COD vial with sink. Invert several sample into the vial. demineralized water times to mix the and wipe the vial...
Page 127 OXYGEN DEMAND, CHEMICAL, continued 2 hours Prepare a blank Heat the vials for Turn the reactor by repeating Steps 3 2 hours. off. Wait 20 minutes to 6, substituting 2.00 for the vials to cool to Note: Many wastewater mL demineralized C or less.
Page 128 OXYGEN DEMAND, CHEMICAL, continued Invert each vial several times while still warm. Place the vials into a rack. Wait until the vials have cooled to room temperature. Note: If a pure green color appears in the reacted sample, the reagent capacity may have been exceeded.
Page 129 COLORIMETRIC DETERMINATION, 0 to 150 mg/L COD Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm concentration units and module number and the zero prompt.
Page 130 Clean the outside Place the sample of the sample vial vial into the adapter The display will with a towel. with the Hach logo count down to 0. facing the front of the Then the display will instrument. show 0.00 mg/L, and the zero prompt will turn off.
Page 131 100-mg/L standard. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagents. Testing 150 mg/L COD concentration samples, the standard deviation was ±5 mg/L COD.
Page 132 COD Reactor, 120/240 Vac ..1 ..each ..45600-00 COD Vial Adapter, DR/700 . . . 1 ..each ..46008-00 DR/700 Filter Module Number 42.01.
Page 133 COD Disposal Information Brochure ......4088 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 42-49...
Page 134 42-50...
Page 135 (0 to 250 mg/L) For palladium-tin activator baths N,N'-Dimethyldithiooxamide Method Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm the concentration and module number units and the zero 42.01 prompt.
Page 136 PALLADIUM, continued 5 minutes Add 0.5 mL of Add the contents Wait 5 minutes. the palladium of one Chromium 1 activator sample to Reagent Powder one of the mixing Pillow to each cylinders (the cylinder. Stopper and prepared sample). invert several times to The other cylinder mix.
Page 137 PALLADIUM, continued Fill a 10-mL Place the blank Press: ZERO cell to the 10-mL line in the cell holder. with the blank. Cap. The display will Note: In bright light, it count down to 0. Then the display will may be necessary to show 0 mg/L and the close the cell zero prompt will turn...
Page 138 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 1.54 mg/L Pd concentration solutions, the standard deviation was ±1.79 mg/L Pd.
Page 139 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 42-55...
Page 140 42-56...
Page 141 Method 8180 PHOSPHORUS, ACID HYDROLYZABLE For water, wastewater, seawater Hydrolysis to Orthophosphate Method* 30 minutes Measure 25 mL of Add 2.0 mL of Place the flask sample into a 50-mL Sulfuric Acid (the prepared sample) erlenmeyer flask Solution, 5.25 N. on a hot plate.
Page 142 PHOSPHORUS, ACID HYDROLYZABLE, continued Cool the prepared Add 2.0 mL of Pour the prepared sample to room 5.0 N Sodium sample into a temperature. Hydroxide Solution. graduated cylinder. Swirl to mix. Add demineralized water rinsings from Note: Use the 1-mL the flask to return the volume to 25 mL.
Page 143 PHOSPHORUS, ACID HYDROLYZABLE continued SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed immediately. If prompt analysis is not possible, samples may be preserved up to 24 hours by cooling to 4 °C (39 °F). Warm to room temperature before testing.
Page 144 Meter, EC10, portable ....each ..50050-00 For Technical Information Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. *Contact Hach for larger sizes. 42-60...
Page 145 (0 to 20 mg/L PO For water and wastewater (also called Orthophosphate) Molybdovanadate Method* Install module Press: After 2 seconds, 42.01 in a DR/700. the display will show The display will show a program number, Note: If samples cannot 420 nm...
Page 146 Then the display will lighting permits the than 24 mg/L, read at show 0.00 mg/L and DR/700 to operate with exactly 3 minutes or the zero prompt will the cell compartment make a 1:1 dilution. turn off.
Page 147 0. Note: Typical indoor Then the display will show the results in lighting permits the mg/L phosphate DR/700 to operate with ). See Table 1 to the cell compartment convert results to cover open. In bright other units.
Page 148 PHOSPHORUS, REACTIVE, continued SAMPLING AND STORAGE Collect samples in clean plastic or glass bottles that have been cleaned with 1:1 Hydrochloric Acid Solution and rinsed with demineralized water. Do not use a commercial detergent because the phosphate content will contaminate the sample. If samples cannot be analyzed the same day, adjust the pH to 2 or less by adding about 2 mL of sulfuric acid, ACS, per liter of sample.
Page 149 PHOSPHORUS, REACTIVE, continued STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and representative lots of testing reagents. Testing 20.00 mg/L PO concentration samples, the standard deviation was ±0.11 mg/L PO Testing zero concentration samples, the limit of detection was 0.27 mg/L...
Page 150 Water, demineralized ..25 mL ..4 L ... . 272-56 REQUIRED APPARATUS DR/700 Filter Module Number 42.01... 1 ..each ..46242-00 OPTIONAL REAGENTS Bromine Water .
Page 151 Spoon, measuring, 0.1 g ....each ..511-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. *Contact Hach for larger sizes 42-67...
Page 152 42-68...
Page 153 Method 8190 PHOSPHORUS, TOTAL For water, wastewater and seawater (also called Organic and Acid Hydrolyzable) Acid Persulfate Digestion Method*; USEPA accepted for reporting Measure 25 mL Add the contents Add 2.0 mL of of sample into a of one Potassium 5.25 N Sulfuric Acid 50-mL erlenmeyer Persulfate Powder...
Page 154 PHOSPHORUS, TOTAL, continued 30 minutes Place the flask on Cool the sample Add 2.0 mL of a hot plate. Boil to room temperature. 5.0 N Sodium gently for 30 minutes. Hydroxide Solution. Swirl to mix. Note: Maintain the Note: Use the 1-mL volume near 20 mL by calibrated dropper adding small amounts of...
Page 155 PHOSPHORUS, TOTAL, continued Pour the sample into a 25-mL graduated cylinder. Using demineralized water rinsings from the flask, return the volume in the cylinder to 25 mL. Proceed with a reactive phosphorus test of the expected total phosphorus concentration range. Note: Results of the reactive phosphorus test at this point will include...
Page 156 PHOSPHORUS, TOTAL, continued SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed immediately. If prompt analysis is not possible, samples may be preserved up to 24 hours by cooling to 4 °C (39 °F). Warm to room temperature before testing.
Page 157 Meter, EC10, portable ....each ..50050-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A - Contact the Hach office or distributor serving you. 42-73...
Page 158 42-74...
Page 159 10 times to mix. Note: If the bath solution has more than 15 g/gal of rhodium a larger dilution is necessary. Note: The DR/700 must be calibrated before samples can be analyzed. See Calibration section. *User calibration required; range is approximate...
Page 160 Mohr pipet. 10 minutes Place the tubes in Install module Press: a COD reactor at 42.01 C for 10 minutes. in a DR/700. The display will show 420 nm and module number 42.01 42-76...
Page 161 Press Hach logo facing to PROGRAM until the display the front of the shows program instrument. number 42.000...
Page 162 0. Then the display will the adapter with the Then the display will show 0.00 and the Hach logo facing to show the results in zero and S1 prompts the front of the mg/L Rh in the will turn off.
Page 163 3750 mg/L, 3.765 g/L or 14.2 g/gal of rhodium in the original bath. When analyzing samples, the DR/700 can display the diluted bath concentration or one of the proportional values. It will display in the same way as it was calibrated.
Page 164 Test Tube Rack, stainless steel ... each ..18641-00 For Technical Assistance, Prices and Ordering In the U.S.A.--Call 800-227-4224 toll-free for more information. Outside the U.S.A.--Contact the Hach Office or distributor serving you. 42-80...
Page 165 For water and wastewater Silicomolybdate Method* Install module Press: After 2 seconds, 42.01 the display will show in a DR/700. The display will show a program number, 420 nm the concentration Note: If samples cannot and module number units and the zero 42.01...
Page 166 SILICA, HR, continued Fill a 10-mL cell Add the contents Add the contents to the 10-mL line of one Molybdate of one Acid Reagent with sample. Reagent Powder Powder Pillow For Pillow For High High Range Silica. Note: A 25-mL sample Range Silica.
Page 167 SILICA, HR, continued Fill a second Place the blank Press: ZERO 10-mL cell to the in the cell holder. 10-mL line with The display will Note: In bright sunlight sample (the blank). count down to 0. Then the display will it may be necessary to show 0.00 mg/L and close the cell...
Page 168 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 20.0 mg/L SiO concentration samples, the standard deviation was ±0.42 mg/L SiO...
Page 169 ..1 ..each ... 968-00 DR/700 Filter Module Number 42.01... 1 ..each ..46242-00...
Page 170 C ... . . each ..1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 42-86...
Page 171 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 172 Table of Contents for 450-nm parameters Barium (user calibration), Sample Cell and AccuVac Ampul ..45-1 Chloride ..........45-13 Color, True and Apparent .
Page 173 45.000 Adjust the pH of samples before analysis. The upper display will show the Note: The DR/700 must concentration of a be calibrated before previous calibration sample analysis. See standard. If desired, Calibration following press the...
Page 174 BARIUM, continued Prepare the Fill a 25-mL cell Fill a 25-mL cell displayed standard. to the 25-mL line to the 25-mL line For each 10 mg/L with the displayed with sample. displayed, pipet 1.00 standard. Cap. mL from a 5000-mg/L Note: Filter highly Barium Voluette colored or turbid water...
Page 175 3. will develop if barium is present. Note: Typical indoor lighting permits the Note: If the BariVer 4 DR/700 to operate with Reagent does not the cell compartment dissolve readily, use a open. In bright 25-mL graduated sunlight, it may be mixing cylinder.
Page 176 Then the display will show 0.00 g/L and the show the results in lighting permits the zero prompt will turn mg/L barium. DR/700 to operate with off. the cell compartment Note: After each test, open. In bright Note: This step adjusts...
Page 177 BARIUM, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds, 45.01 the display will show in a DR/700. The display will show a program number, 450 nm the concentration and module number units and the zero 45.01 prompt. Press...
Page 178 BARIUM, continued Prepare the Collect at least Fill a Barium displayed standard. 40 mL of sample in a AccuVac Ampul with For each 10 mg/L 50-mL beaker. the displayed displayed, pipet Collect at least 40 mL standard. Fill a 1.00 mL from a of the displayed second AccuVac 5000-mg/L Barium...
Page 179 Note: This step adjusts the DR/700 to the calibration curve previously entered. Press: READ The display will count down to 0.
Page 180 Process each in the same way as the "displayed standard" described in this method. Next, perform paragraph 3.2.4.1, Calibration Using Two Prepared Standards, in the DR/700 instrument manual. Either standard can be used as Standard 1 or Standard 2. 45-8...
Page 181 ..1 ..each ... . 968-00 DR/700 Filter Module Number 45.01... 1 ..each ..46245-00...
Page 182 BARIUM, continued REQUIRED APPARATUS (Using Powder Pillows) (cont.) Quantity Description Per Test Unit Cat. No. Flask, volumetric, 500 mL . . . 1 ..each ..14574-49 Pipet, Mohr, 25 mL .
Page 183 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial for DR/700..each ..46025-00 Brush .
Page 184 45-12...
Page 185 Install module Press: After 2 seconds, number the display will show 45.01 The display will show a program number, in a DR/700. 455 nm the concentration and module number units and the zero Note: Samples can be 45.01 prompt. If necessary,...
Page 186 Cap Note: Typical indoor each cell and invert several times to mix. lighting permits the DR/700 to operate with Note: An orange color the cell compartment will develop if chloride open. In bright sunlight, is present.
Page 187 Chloride Standard Solution, 1000 mg/L, to 500 mL with demineralized water. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 12.0 mg/L Cl concentration samples, the standard deviation was ±0.34 mg/L Cl Testing zero concentration samples, the limit of detection was 0.14 mg/L...
Page 188 Water, demineralized ..25 mL ..4 L ... . . 272-56 REQUIRED APPARATUS DR/700 Filter Module Number 45.01... 1 ..each ..46245-00 Pipet, volumetric, 1.0 mL .
Page 189 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 50 tests equals 25 samples and 25 blanks. 45-17...
Page 190 45-18...
Page 191 Sampling and Storage following these steps. Fill a 10-mL cell Install module Press: to the 10-mL line 45.01 with filtered in a DR/700. The display will show demineralized water 450 nm (the blank). Discard and module number the excess. 45.01...
Page 192 COLOR, TRUE AND APPARENT, continued After 2 seconds, Pour about 50 mL Fill a second the display will show of sample through the 10-mL cell to the a program number, filter. 10-mL line with the the concentration filtered sample (the units and the zero prepared sample).
Page 193 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 500 color units concentration solutions, the standard deviation was ±7.0 color units.
Page 194 Aspirator, vacuum ..1 ..each ... 2131-00 DR/700 Filter Module Number 45.01... 1 ..each ..46245-00 Filter Holder, 47 mm, 300 mL graduated .
Page 195 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 45-23...
Page 196 45-24...
Page 197 For boiler water/feedwater, water and seawater p-Dimethylaminobenzaldehyde Method* Install module Press: After 2 seconds, 45.01 the display will show in a DR/700. The display will show the program number, 450 nm the concentration Note: Samples must be and module number units, decimal 45.01...
Page 198 HYDRAZINE, continued Pour 10.0 mL of Pour 10.0 mL of Add 0.5 mL of demineralized water sample into a second HydraVer 2 into a 10-mL sample 10-mL sample cell Hydrazine Reagent to cell (the blank) using (the prepared sample) each sample cell. a graduated cylinder.
Page 199 Use the working solution in place of the sample in Step 5. The result should be 100 µg/L hydrazine. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two 45-27...
Page 200 Cylinder, graduated, 25 mL . . . 1 ..each ... . 508-40 DR/700 Filter Module Number 45.01... 1 ..each ..46245-00 OPTIONAL REAGENTS Hydrazine Sulfate, ACS .
Page 201 Thermometer, -20 to 105 °C ... . . each ..1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A - Contact the Hach Office or distributor serving you. *Contact Hach for larger sizes. 45-29...
Page 202 45-30...
Page 203 Method 8047 PHENOLS (0 to 0.200 mg/L) For water, wastewater and seawater 4-Aminoantipyrine Method*, USEPA accepted for reporting (distillation is required; see Section 1)** Measure 300 mL Pour the Measure 300 mL of demineralized measured of sample in a 500-mL water in a 500-mL demineralized water graduated cylinder.
Page 204 PHENOLS, continued 30 seconds Add the contents Add 30 mL of Holding the of one Phenol 2 chloroform to each stopper in, invert each Reagent Powder separatory funnel. funnel and Pillow to each Stopper each funnel. temporarily vent. separatory funnel. Shake each funnel Stopper.
Page 205 PHENOLS, continued Remove the Insert a pea-size Drain the stoppers. Allow both cotton plug into the chloroform layers funnels to stand until delivery tube of each into separate sample the chloroform settles funnel. cells - one for the to the bottom of the blank and one for the funnel.
Page 206 Note: Typical indoor Then the display will Note: Typical indoor lighting permits the show 0.000 mg/L. lighting permits the DR/700 to operate with DR/700 to operate with the cell compartment the cell compartment cover open. In bright cover open. In bright...
Page 207 PHENOLS, continued Press: READ The display will count down to 0. Then the display will show the results in mg/L phenols. SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed within four hours after collection. The following storage instructions are necessary only when prompt analysis is impossible.
Page 208 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 0.100 mg/L phenols concentration solutions, the standard deviation was ±0.0047 mg/L phenols.
Page 209 PHENOLS, continued necessary to elevate the flask with a laboratory jack. Place a stirring bar into the distillation flask. b) Measure 300 mL of sample in a 500-mL graduated cylinder. Pour it into the distillation flask. c) Using a serological pipet, add 1 mL of Methyl Orange Indicator Solution to the distillation flask.
Page 210 Cylinder, 500 mL graduated . . . 1 ..each ... . 508-49 DR/700 Filter Module Number 45.01... 1 ..each ..46245-00 Funnel, 500 mL separatory .
Page 211 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 45-39...
Page 212 45-40...
Page 213 45.01 turbid or colored samples. Note: If samples cannot be analyzed immediately, see Sampling and Storage following these steps. Note: The DR/700 must be calibrated before sample analysis. See Calibration following these steps. *User calibration required; range is approximate. 45-41...
Page 214 POTASSIUM, continued After 2 seconds, Make the Fill a 25-mL the display will show displayed standard. graduated mixing a program number, For each mg/L cylinder with 25 mL the concentration displayed, pipet 1 mL of the displayed units, decimal from a 250-mg/L standard.
Page 215 Cap each cell. Note: Typical indoor Then the display will show the lighting permits the concentration of the DR/700 to operate with standard and the zero the cell compartment prompt will turn off. cover open. In bright light, it may be necessary to close the cell compartment cover.
Page 216 Make each as described in step 5 of the potassium procedure. Continue by doing steps 2 and 6 through 10, processing both standards like the displayed standard. Next, perform paragraph 3.2.4.1, Calibration Using Two Prepared Standards, in the DR/700 Instrument Manual. Either standard can be 45-44...
Page 217 POTASSIUM, continued used as Standard 1 or Standard 2. Complete the calibration within 7 minutes after the 3-minute period. SAMPLING AND STORAGE Collect samples in acid-washed plastic bottles. Adjust the pH to 2 or less with nitric acid (about 2 mL per liter). Preserved samples may be stored at least six months at room temperature.
Page 218 25 mL ..1 ..each ... 1896-40 DR/700 Filter Module Number 45.01... 1 ..each ..46245-00 OPTIONAL REAGENTS Nitric Acid, ACS .
Page 219 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 45-47...
Page 220 45-48...
Page 221 For water, wastewater and seawater Direct Colorimetric Method Install module Press: After 2 seconds, 45.01 the display will show in a DR/700. The display will show a program number, 450 nm the concentration Note: If samples cannot and module units, decimal 45.01...
Page 222 0. Then the display will Note: Typical indoor lighting permits the show 0.00 mg/L and lighting permits the DR/700 to operate with the zero prompt will DR/700 to operate with the cell compartment turn off. the cell compartment cover open.
Page 223 Large amounts of turbidity will result in high readings. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 934 mg/L Na concentration samples, the standard deviation was ±5.4 mg/L Na Testing zero concentration samples, the limit of detection was 8.1 mg/L...
Page 224 Sample Cell, 10-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A.-Call 800-227-4224 toll-free for more information. Outside the U.S.A.-Contact the Hach office or the distributor serving you. 45-52...
Page 225 PROGRAM Sampling and Storage or twice until the following these steps. lower display shows program number Note: The DR/700 must 45.000 be calibrated before sample analysis. See The upper display Calibration following will show the these steps.
Page 226 SULFATE, continued Prepare the Fill a 10-mL cell Fill a 10-mL cell displayed standard. to the 10-mL line to the 10-mL line For each mg/L with the displayed with sample. displayed, pipet 1.00 sample. Cap. mL of 1000 mg/L of Note: Filter highly Sulfate Standard colored or turbid...
Page 227 SULFATE, continued 5 minutes Add the contents Wait 5 minutes. Place the prepared of one SulfaVer 4 standard into the cell Sulfate Reagent holder. Note: Allow cells to Powder Pillow to stand undisturbed. each sample cell. Note: In bright sunlight Cap and invert several it may be necessary to Note: Perform Steps 9-...
Page 228 PROGRAM immediately, see key once or twice Sampling and Storage, until the lower below. display shows program number Note: The DR/700 must 45.000 be calibrated before sample analysis. See The upper display Calibration following will show the these steps. concentration of a previous calibration standard.
Page 229 SULFATE, continued Prepare the Pour at least 40 Collect at least displayed standard. mL of the displayed 40 mL of sample in a For each mg/L standard into a 50-mL beaker. displayed, pipet 50-mL beaker. 1.00 mL of 1000 Note: Filter highly mg/L of Sulfate colored or turbid Standard Solution...
Page 230 SULFATE, continued 5 minutes Fill a SulfaVer 4 Quickly invert the Wait 5 minutes. Sulfate AccuVac sample several time Ampul with to mix. Wipe off any Note: Allow the ampul displayed standard. liquid or fingerprints. to stand undisturbed. Fill a second AccuVac Ampul with Note: A white turbidity Note: Complete Steps...
Page 231 SULFATE, continued Place the Within five prepared sample in minutes after the five- the cell holder. minute waiting period, press: READ Note: In bright sunlight The display will it may be necessary to count down to 0. close the cell Then the display will compartment cover.
Page 232 Process each on the same way as the "displayed standard" described in the test procedure. Next perform paragraph 3.2.4.1, Calibration Using Two Prepared Standards, in the DR/700 instrument manual. Either standard can be used as Standard 1 (S1) or Standard 2 (S2). INTERFERENCES...
Page 233 ..1 ..each ... 968-00 DR/700 Filter Module Number 45.01... 1 ..each ..46245-00 Flask, volumetric, 1000 mL .
Page 234 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..43784-00 Ampule Breaker Kit .
Page 235 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 236 Table of Contents for 480-nm parameters Lead, LeadTrak ......... 48-1...
Page 237 Method 8317 (0 to 150 µg/L) LEAD For drinking water ® LeadTrak Fast Column Extraction Method 2 minutes Fill a 100-mL Using a plastic Wait 2 minutes. 1-mL dropper, add plastic graduated 1.0 mL of pPb-1 Acid cylinder with Preservative Solution 100 mL of the water to the sample and to be tested.
Page 238 LEAD, continued Use a second Mount a new Fast Pour the prepared 1-mL plastic dropper Column Extractor in sample slowly into to add 2.0 mL of a ring stand with a the Column pPb-2 Fixer Solution clamp. Place a Extractor. Wait for to the sample.
Page 239 LEAD, continued After the flow has Place a 25-mL After the Eluant stopped, fully sample cell under the Solution has started compress the Extractor. Using a to drip from the adsorbent pad in the 25-mL plastic Extractor, insert the Extractor with the graduated cylinder, plunger and slowly plunger.
Page 240 Note: The solution will the next step. turn brown. Install module Press: After 2 seconds, 48.01 the display will show in a DR/700. The display will show a program number, 480 nm concentration units, and module number decimal position and 48.01 the zero prompt.
Page 241 Note: Typical indoor drops of pPb-6 Then the display will lighting permits the Decolorizer Solution show 0.00 µg/L and DR/700 to operate with to the cell. Cap and the zero prompt will the cell compartment invert several times to turn off.
Page 242 0. Then the display will Note: Typical indoor show the results in lighting permits the µg/L lead (Pb). DR/700 to operate with the cell compartment Note: Running a cover open. In bright reagent blank with lead- sunlight it may be...
Page 243 LEAD, continued APPARATUS/SAMPLE PREPARATION Because lead is very common to our environment, care must be taken to prevent sample contamination. Follow these steps for greatest test accuracy: a) Lead-free water is necessary to minimize sample contamination when rinsing apparatus or diluting sample. The water may be either distilled or demineralized.
Page 244 LEAD, continued c) After two minutes the sample is ready for analysis. Steps 2 and 3 are skipped in the analysis procedure. Use 100 mL of this preserved sample directly in Step 7. Sampling for lead contamination from drinking water sources such as well water or water from main supply lines a) Add 10 mL of pPb-1 Acid Preservative to a one-liter bottle.
Page 245 LEAD, continued b) Swirl the sample to mix. Then test the sample as described in the procedure. Each 0.1-mL of standard added should increase the lead concentration determined in Step 20 by 10 µg/L. Standard Solution Method A 50-µg/L lead standard solution can be prepared by first pipetting 1.00 mL of Lead Standard Solution, 1000 mg/L as Pb, into a 100-mL plastic volumetric flask and diluting to the mark with lead-free water to make a 10 mg/L lead working solution (included in the reagent set).
Page 246 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched cells and two representative lots of testing reagents. Testing 100 µg/L Pb concentration samples the standard deviation was ±3.0 µg/L Pb.
Page 247 25 mL ..1 ..each ... 1081-40 DR/700 Filter Module Number 48.01... 1 ..each ..46248-00 Support, ring stand .
Page 248 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 48-12...
Page 249 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 250 Table of Contents for 500-nm parameters Cyanuric Acid (user calibration) ......50-1 Iron, Ferrous, Sample Cell and AccuVac Ampul ....50-7 Iron, Total, FerroVer, Sample Cell and AccuVac Ampul.
Page 251 Use the labware listed under Optional Apparatus. Large amounts of color or turbidity will interfere and cause high readings. Note: The DR/700 must be calibrated before sample analysis. See Calibration following these steps. *User calibration required. Range is approximate. 50-1...
Page 252 CYANURIC ACID, continued After 2 seconds, Make the Fill a 25-mL cell the display will show displayed standard. to the 25-mL line a program number, For each mg/L with the displayed the concentration displayed, pipet standard. units and the zero 1.00 mL from a prompt.
Page 253 Cap and invert several Note: Typical indoor after the 3-minute times to mix. lighting permits the waiting period. DR/700 to operate with Note: A white turbidity the cell compartment will form if cyanuric open. In bright acid is present. sunlight, it may be necessary to close the cell compartment cover.
Page 254 Note: Typical indoor show the The display will lighting permits the concentration of the count down to 0. DR/700 to operate with standard and the zero Then the display will the cell compartment prompt will turn off. show the results in open.
Page 255 Perform Steps 6-8 with both these standards. d) With Filter Module 51.01 installed, perform paragraph 3.2.4.1, Calibration Using Two Prepared Standards, in the DR/700 instrument manual. Either standard can be used as Standard 1 or Standard 2. Calibration must be completed within 7 minutes after the 3-minute reaction period.
Page 256 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 50-6...
Page 257 (Powder Pillows or AccuVac Ampuls) USING POWDER PILLOWS Install module Press: After 2 seconds, 50.01 the display will show in a DR/700. The display will show a program number, 500 nm concentration units Note: Analyze samples and module number and the zero prompt.
Page 258 IRON, FERROUS, continued 3 minutes Fill a 25-mL cell Add the contents Wait 3 minutes. to the 25-mL line of one Ferrous Iron with sample. Reagent Powder Note: Steps 7 and 8 can Pillow to the sample be completed during Note: For proof of cell (the prepared this reaction period.
Page 259 IRON, FERROUS, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Note: In bright light, Then the display will show the results in put 10 mL in a 10-mL mg/L ferrous iron cell, insert it and close the cell compartment cover.
Page 260 IRON, FERROUS, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds, 50.01 the display will show in a DR/700. The display will show a program number, 500 nm concentration units Note: Analyze samples and module number and the zero prompt.
Page 261 IRON, FERROUS, continued 3 minutes Wait 3 minutes. Place the blank in Press: ZERO the cell holder. The display will Note: In bright light count down to 0. then the display will show close the cell 0.00 mg/L and the compartment cover.
Page 262 1.0 mg/L standard solution. Prepare this immediately before use. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.50 mg/L Fe concentration samples, the standard deviation was ±0.008 mg/L Fe Testing zero concentration samples, the limit of detection was 0.007 mg/L...
Page 263 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Cap for 10- and 25-mL sample cells .
Page 264 50-14...
Page 265 USEPA approved for reporting (digestion - see Section 1)** USING POWDER PILLOWS Install module Press: After 2 seconds 50.01 the display will show in a DR/700. The display will show a program number, 500 nm concentration units, Note: If samples cannot and module number decimal position and 50.01...
Page 266 IRON, TOTAL, continued 3 minutes Fill a 10-mL cell Add the contents Wait 3 minutes. to the 10-mL line of one FerroVer Iron with sample. Reagent Powder Note: Samples Pillow to the sample containing visible rust Note: A 25-mL sample cell (the prepared should be allowed to sample).
Page 267 IRON, TOTAL, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Note: In bright sunlight Then the display will show the results in it may be necessary to mg/L iron (Fe). close the cell compartment cover.
Page 268 IRON, TOTAL, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds 50.01 the display will show in a DR/700. The display will show a program number, 500 nm concentration units, Note: If samples cannot and module number decimal position and 50.01...
Page 269 IRON, TOTAL, continued 3 minutes Wait 3 minutes. Within 30 minutes Press: ZERO after the 3-minute Note: Samples period, place the The display will blank in the cell count down to 0. containing visible rust holder. Then the display will should be allowed to show 0.000 mg/L and react at least five...
Page 270 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.5 mg/L Fe concentration samples, the standard deviation was ±0.008 mg/L Fe Testing zero concentration samples, the limit of detection was 0.007 mg/L Fe...
Page 271 IRON, TOTAL, continued standard deviation when testing zero concentration samples (Adapted from Analytical Chemistry, 1980, 52, 2242-2249). Using two representative lots of FerroVer Iron AccuVac Ampuls, the standard deviation was ±0.012 mg/L Fe and the limit of detection was 0.014 mg/L Fe INTERFERENCES The following will not interfere below the levels shown: Chloride...
Page 272 REQUIRED APPARATUS (Using Powder Pillows) DR/700 Filter Module Number 50.01... 1 ..each ..46250-00 Clippers, for opening powder pillows .
Page 273 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac vial, DR/700 ..each ..46025-00 Ampule Breaker Kit .
Page 274 50-24...
Page 275 Install module Press: After 2 seconds, number the display will show 50.01 The display will show a program number, in a DR/700. 500 nm the concentration and module number units, decimal Note: If sample cannot 50.01 position and the zero prompt.
Page 276 NITRATE, HR, continued 1 minute Fill a 10-mL cell Add the contents Shake the cell to the 10-mL line of one NitraVer 5 vigorously for one with sample. Reagent Powder minute. Pillow to the cell (the prepared sample). Note: A deposit of Cap.
Page 277 NITRATE, HR, continued 5 minutes Set the cell down Fill a 10-mL cell Place the blank in and wait 5 minutes. to the 10-mL line the cell holder. with sample (the Note: Do not wait more Note: In bright sunlight blank).
Page 278 NITRATE, HR, continued Press: Place the Press: ZERO READ prepared sample in The display will the cell holder. The display will count count down to 0. down to 0. Then the Then the display will Note: In bright sunlight display will show the show 0.0 mg/L and results in mg/L nitrate it may be necessary to...
Page 279 Install module Press: After 2 seconds number the display will show 50.01 The display will show a program number, in a DR/700. 500 nm the concentration and module number units, decimal Note: If sample cannot 50.01 position and the zero prompt.
Page 280 NITRATE, HR, continued 5 minutes Wait 5 minutes. Place the blank in Press: ZERO the cell holder. The display will Note: A deposit of Note: In bright sunlight count down to 0. unoxidized metal will Then the display will it may be necessary to remain after the show 0.0 mg/L and close the cell...
Page 281 NITRATE, HR, continued Place the Place the Press: READ AccuVac Vial prepared sample in Adapter into the cell the cell holder. The display will holder. count down to 0. Note: In bright sunlight Then the display will show the results in it may be necessary to mg/L nitrate as close the cell...
Page 282 PRECISION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 30.0 mg/L NO concentration samples, the standard deviation was ±3.40 mg/L NO Testing zero concentration samples, the limit of detection was 0.66 mg/L...
Page 283 (i.e., seawater), but a calibration must be performed using standards spiked to the same chloride concentration. See Calibrations paragraph 3.2.4 in the DR/700 Instrument Manual for more information. Highly buffered samples or extreme sample pH may exceed the buffering capacity of the reagents and require sample pretreatment; see Interferences, pH (Section I).
Page 284 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Cap for 10-and 25-mL sample cells .
Page 285 Install module Press: After 2 seconds, number the display will show 50.01 The display will show a program number, in a DR/700. 500 nm the concentration and module number units, decimal Note: If samples cannot 50.01 position and the zero prompt.
Page 286 NITRATE, LR, continued 3 minutes Fill a 50-mL Add the contents Shake the cylinder graduated mixing of one NitraVer 6 continuously for three cylinder to the 30-mL Nitrate Reagent minutes. line with sample. Powder Pillow to the cylinder. Stopper. Note: A deposit of Note: For proof of unoxidized metal will accuracy, use a 0.20 mg/L...
Page 287 NITRATE, LR, continued 2 minutes Wait 2 minutes. Fill a 10-mL cell Add the contents to the 10-mL line of one NitriVer 3 with the prepared Nitrite Reagent Note: A two-minute sample. Powder Pillow to the period allows cadmium sample cell (the to settle.
Page 288 NITRATE, LR, continued Press: Place the Press: ZERO READ prepared sample in The display will the cell holder. The display will count down to 0. count down to 0. Then the display will Note: In bright sunlight, Then the display will show 0.00 mg/L and show the results in it may be necessary to...
Page 289 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.30 mg/L NO concentration samples the standard deviation was ±0.012 mg/L NO...
Page 290 Chlorides in amounts over 100 mg/L cause low results. To determine nitrate in high chloride samples or seawater, a manual calibration must be performed. See section 3.2.4 in the DR/700 instrument manual. Prepare nitrate standard solutions with the approximate chloride concentration of the samples to be tested.
Page 291 50 mL ..1 ..each ... 1896-41 DR/700 Filter Module Number 50.01... 1 ..each ..46250-00 OPTIONAL REAGENTS Bromine Water .
Page 292 Nitrate at these levels can be determined directly using the Nitrate Ion Selective Electrode (Cat. No. 44560-71) For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 50-42...
Page 293 Install module Press: After 2 seconds, number the display will show 50.01 The display will show a program number, in a DR/700 500 nm the concentration and module number units, decimal Note: If sample cannot 50.01 position and the zero prompt.
Page 294 NITRITE, LR, continued 10 minutes Fill a 10-mL cell Add the contents Wait 10 minutes. to the 10-mL line of one NitriVer 3 with sample (the Nitrite Reagent blank). Powder Pillow (the prepared sample). Note: For proof of Cap and invert to mix.
Page 295 NITRITE, LR, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Note: In bright sunlight Then the display will show the results in it may be necessary to mg/L nitrite as close the cell nitrogen (NO -N).
Page 296 NITRITE, LR, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds, 50.01 the display will show in a DR/700. The display will show a program number, 500 nm the concentration Note: If sample cannot and module number units, decimal 50.01...
Page 297 NITRITE, LR, continued 10 minutes Wait 10 minutes. Place the blank in Press: ZERO the cell holder. The display will count down to 0. Note: In bright sunlight Then the display will it may be necessary to show 0.000 mg/L and close the cell the zero prompt will compartment cover.
Page 298 Prepare this solution immediately before use. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.091 mg/L NO -N concentration samples the standard deviation was ±0.0010 mg/L NO...
Page 299 ..1 ..each ... 968-00 DR/700 Filter Module Number 50.01... 1 ..each ..46250-00 REQUIRED APPARATUS (Using AccuVac Ampuls) Adapter, AccuVac Vial .
Page 300 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Balance, analytical .
Page 301 Note: If the platinum bath solution has more than 10 g/L (1.2 troy oz./gal) of platinum, a larger dilution is necessary. Note: The DR/700 must be calibrated before sample measurement. See Calibration following these steps. *User calibration required; range is approximate.
Page 302 PLATINUM, continued Add the contents Place both bottles Cool both bottles of one Chromium 1 in a boiling water under tap water to Reagent Powder bath for five minutes. about room Pillow to each bottle. temperature. Swirl to dissolve. Note: The sample may Place a hollow become turbid upon polyethylene stopper...
Page 303 PLATINUM, continued 5 minutes Wait 5 minutes. Install module Press: 50.01 in a DR/700. The display will show 500 nm and module number 50.01 After 2 seconds, Fill a 10-mL cell After the 5- the display will show to the 10-mL line...
Page 304 PLATINUM, continued Place the blank Press: Place the ZERO in the cell holder. prepared sample in The display will the cell holder. Note: In bright light it count down to 0. Then the display will Note: In bright light it may be necessary to show zero may be necessary to...
Page 305 Stopper. Invert at least 10 times to mix. Refer to paragraph 2.2.4.1, Calibration Using Two Prepared Standards, in the DR/700 instrument manual. Follow the platinum procedure starting at Step 2 to develop the S1 and S2 standards. Use 15 mL of demineralized water in Step 3.
Page 306 (requires hot plate) ..1 ..each ... 1955-55 For Technical assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 50-56...
Page 307 Method 8107 POLYACRYLIC ACID (0 to 20.0 mg/L) For water and brines ® Adsorption-Colorimetric Method (as Acrysol LMW-20, -45) SAMPLE PREPARATION Remove the Rinse the syringe Insert the plunger syringe plunger. with the sample. Fill and force the sample Attach the prefilter to to the 30-cc mark.
Page 308 POLYACRYLIC ACID, continued Lever Lever Lever Assemble the Rinse the syringe. Draw the syringe apparatus as Discard the solution remaining reagent shown. Place the through the tubing. blank into the syringe long end of the LC through the tubing cartridge on the male Note: Move the plunger followed by a small tip of the three-way...
Page 309 POLYACRYLIC ACID, continued Lever Lever Lever Rinse the Draw at least 10 Push the plunger syringe by shaking. cc of eluant into the down to adjust the Discard the eluant syringe through the eluant volume to through the tubing. tubing followed by exactly the 10-cc air past the 25-cc mark.
Page 310 POLYACRYLIC ACID, continued Lever Lever Repeat, Return the valve discarding the water to the aspirate (down) through the cartridge position and repeat with the valve in the Steps 8 through 20, pump (left) position. using the buffered sample in place of the Note: The cartridge blank.
Page 311 Install module Press: After 2 seconds, number the display will show 50.01 The display will show a program number, in a DR/700. 500 nm and module the concentration number units, decimal 50.01 position and the zero prompt. If necessary, press the...
Page 312 POLYACRYLIC ACID, continued 5 minutes Immediately After the 5- Place the cell place the cylinders in minute period, fill a labeled "reagent the dark. Begin 10-mL cell to the blank" in the cell timing a 5-minute 10-mL line with the holder.
Page 313 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 3.4 mg/L Acrysol LMW- 45, acid form, concentration samples the standard deviation was ±0.33 mg/L pAA.
Page 314 POLYACRYLIC ACID, continued INTERFERENCES Filter turbid or oily samples through glass wool or a moderately rapid paper, such as S&S No. 560, before beginning the test. If the sample is laden with oil, do a preliminary extraction of the oil with 1,1,2-trichloro-1,2,2-trifluoroethane (Freon 113) as follows: a) Transfer a sample portion to a clean 125-mL separatory funnel.
Page 315 25 mL ..2 ..each ..21190-40 DR/700 Filter Module Number 50.01... 1 ..each ..46250-00 Flask, erlenmeyer, 50 mL .
Page 316 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A - Contact the Hach office or distributor serving you. 50-66...
Page 317 Method 8196 VOLATILE ACIDS (0 to 2500 mg/L) For digestor sludges Esterification Method* Pipet 0.5 mL of Filter or Pipet 0.5 mL of demineralized water centrifuge 25 mL of the filtrate or into a dry 25-mL the sample using supernatant into square mixing bottle labware listed under another dry 25-mL...
Page 318 VOLATILE ACIDS, continued 3 minutes Wait 3 minutes. Cool solutions to Pipet 0.5 mL of C (until the cell Hydroxylamine feels cold) with Hydrochloride running tap water. Solution into each cell. Swirl to mix. Pipet 2.0 mL of Add 10 mL of Add 10 mL of 4.5 N Sodium Ferric Chloride...
Page 319 VOLATILE ACIDS, continued 3 minutes Wait 3 minutes. Install module Press: 50.01 Note: During the 3- in a DR/700. The display will show 500 nm minute period, complete and module number Step 14-20 so the 50.01 prepared sample is read when the timer stops.
Page 320 VOLATILE ACIDS, continued Press: Pour 10 mL of Place the ZERO the prepared sample prepared sample in The display will into a 10-mL round the cell holder. count down to 0. sample cell. Cap. Then the display will Note: In bright light, it show 0 mg/L and the may be necessary to zero prompt will turn...
Page 321 100-mL volumetric flask. Dilute to volume with demineralized water. STATISTICAL EVALUATION A single operator repetitively tested samples of two prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1250 mg/L as HOAc concentration samples, the standard deviation was ±4.5 mg/L HOAc.
Page 322 Cylinder, graduated, 10 mL. . . 1 ..each ... . 508-38 DR/700 Filter Module Number 50.01... 1 ..each ..46250-00 Hot Plate, 4-inch circular, 3-1/2"...
Page 323 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 50-73...
Page 324 50-74...
Page 325 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 326 Table of Contents for 525-nm parameters Aluminum, Aluminon ........52-1 Aluminum, ECR .
Page 327 Method 8012 ALUMINUM (0 to 1.00 mg/L) For water and wastewater Aluminon Method* Fill a 50-mL Add the contents Add the contents graduated mixing of one Ascorbic Acid of one AluVer 3 cylinder to the 50-mL Powder Pillow. Aluminum Reagent mark with sample.
Page 328 ALUMINUM, continued Fill a 25-mL cell Add the contents Pour the to the 25-mL line of one Bleaching 3 remaining 25 mL of with the mixture (the Reagent Powder bleached sample in prepared sample). Pillow to the the cylinder into a Cap.
Page 329 Note: Typical indoor and the zero prompt display shows will turn off. lighting permits the program number DR/700 to operate with 52.01.1 the cell compartment open. In bright sunlight, it may be necessary to close the cell compartment cover.
Page 330 0. Then the display will show the results in Note: Typical indoor mg/L aluminum. lighting permits the DR/700 to operate with Note: Clean the the cell compartment graduated cylinder and open. In bright sunlight, sample cells with soap...
Page 331 Dilute to volume with demineralized water. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 0.50 mg/L Al concentration solutions, the standard deviation was ±0.009 mg/L Al.
Page 332 ALUMINUM, continued 0.008 mg/L Al. The limit of detection was calculated as three times the standard deviation when testing zero concentration samples (adapted from Analytical Chemistry, 1980, 52, 2242-2249). INTERFERENCES The following do not interfere up to the indicated concentrations. Alkalinity 1000 mg/L as CaCO Iron...
Page 333 Ascorbic acid is added to remove iron interference. The AluVer 3 Aluminum Reagent, packaged in powder form shows exceptional stability and is applicable for fresh water samples. DISPLAYED DR/700 VALUE (mg/L Al mg/L AL TRUE ALUMINUM VALUE FLUORIDE INTERFERENCE GRAPH...
Page 334 50 mL ....1..each ..1896-41 DR/700 Filter Module Number 52.01....1..each ..46252-00 OPTIONAL REAGENTS Aluminum Standard Solution, 100 mg/L .
Page 335 C....each ..1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 52-9...
Page 336 52-10...
Page 337 For water Eriochrome Cyanine R Method* Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm the concentration and module units and the zero Note: If samples cannot 52.01...
Page 338 ALUMINUM, continued Fill a 50-mL Add the contents Add the contents graduated mixing of one ECR Reagent of one Hexa- cylinder to the 50-mL Powder Pillow. methylenetetramine mark with sample. Stopper and invert Buffer Reagent several times to Powder Pillow. dissolve the powder;...
Page 339 ALUMINUM, continued 5 minutes Put 1 drop of ECR Fill the sample Wait 5 minutes. Masking Reagent cell to the 10-mL Solution into a 10-mL mark with the sample cell. solution from the cylinder (the blank). Cap and invert several times to mix.
Page 340 ALUMINUM, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Then the display will Note: In bright light, it show the results in may be necessary to mg/L aluminum. close the cell compartment cover.
Page 341 Al reading in Step 14 should be 0.10 mg/L Al. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 0.200 mg/L Al concentration solutions, the standard deviation was ±0.0049 mg/L Al.
Page 342 ALUMINUM, continued Table 1. Common Interferences with the Eriochrome Cyanine R Method Constituent Concentration Error Acidity 0-62 mg/L as CaCO Alkalinity 0-750 mg/L as CaCO 0-1000 mg/L as CaCO 0-1000 mg/L 0.2 mg/L -5% of reading 2 mg/L -5% of reading 0-4 mg/L + mg/L Fe 0.0075...
Page 343 ALUMINUM, continued used in the following pretreatment are replaced, even if the new reagent has a matching lot number. When the pretreated sample has been analyzed, subtract the aluminum concentration of the reagent blank from the sample results. c) Measure 50 mL of sample into the 125-mL erlenmeyer flask using the graduated cylinder.
Page 344 ALUMINUM, continued Table 2. True aluminum concentration (mg/L) vs. DR/700 reading (mg/L) and fluoride concentration (mg/L) with the Eriochrome Cyanine R method DR/700 Fluoride Concentration (mg/L) Reading (mg/L) 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000...
Page 345 ... . 1..each ..1896-41 DR/700 Filter Module Number 52.01 ..1..each ..46252-00 OPTIONAL REAGENTS Aluminum Standard Solution, 100 mg/L .
Page 346 C ....each ..1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 52-20...
Page 347 Prepare and store the calibration as directed under Using User-Programmed Method (paragraph 3.2.4) in the DR/700 Instrument Manual. Then use the following procedure. †User calibration required; range is approximate. *Adapted from Standard Methods for the Examination of Water and Wastewater **Procedure is equivalent to USEPA method 206.4 for wastewater and Standard Method...
Page 348 ARSENIC, continued Stir control: 5 Heat control: 0 Measure 250 mL Turn on the power Measure 25 mL of of sample into the switch. Set the stir hydrochloric acid, distillation flask control to 5. Set the ACS, into the flask using a graduated heat control to 0.
Page 349 ARSENIC, continued 15 minutes 15 minutes Add 6.0 g of Set the heat Set the heat 20-mesh zinc to the control to 3. Wait 15 control to 1. Wait 15 flask. Cap minutes. minutes. immediately. Note: Use an analytical balance to weigh the zinc metal.
Page 350 0. and the zero prompt. Then the display will lighting permits the Press the show the PROGRAM DR/700 to operate with key until the display concentration of the the cell compartment shows program standard and the zero open. In bright...
Page 351 0. Then the display will Note: Typical indoor show the results in lighting permits the mg/L arsenic (As). DR/700 to operate with the cell compartment open. In bright sunlight, it may be necessary to close the cell compartment cover.
Page 352 Perform Steps 1-18 of the arsenic procedure using the 0.20 mg/L arsenic standard. d) Perform the instructions in the "Calibration Using Two Prepared Standards" section of the DR/700 Instrument Manual (paragraph 3.2.4.1). For Standard 1, use the blank from the arsenic procedure and make the display read 00.00 mg/L.
Page 353 Purpose Accessories ..1 ..set ... 22653-00 DR/700 Module Number 52.01... 1 ..each ..46252-00 Flask, volumetric, 100 mL .
Page 354 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 52-28...
Page 355 DPD Method* (Powder Pillows or AccuVac Ampuls) USING POWDER PILLOWS Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm the concentration Note: Samples must be and module number units and the zero 52.01...
Page 356 BROMINE, continue 3 minutes Fill a 10-mL Add the contents Wait 3 minutes. sample cell to the of one DPD Total 10-mL line with Chlorine Powder sample. Pillow to the sample cell (the prepared Note: A 25-mL sample sample). Cap and shake the cell for 20 can be tested by using seconds.
Page 357 BROMINE, continue Fill a 10-mL cell Place the blank in Press: ZERO to the 10-mL line the cell holder. with sample (the The display will blank). Cap. Note: In bright light it count down to 0. Then the display will may be necessary to show 0.00 mg/L and close the cell...
Page 358 USING ACCUVAC AMPULS Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm the concentration Note: Sample must be and module number units and the zero 52.01...
Page 359 BROMINE, continue 3 minutes Wait 3 minutes. Place the blank in Press: ZERO the cell holder. The display will Note: In bright light it count down to 0. Then the display will may be necessary to show 0.00 mg/L and close the cell the zero prompt will compartment cover.
Page 360 If these increases do not occur, see Standard Additions (Section I) for more information. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 1.00 mg/L Cl (equivalent to 2.25 mg/L Br ) concentration solutions, the standard deviation was ±0.005 mg/L CL...
Page 361 Adapter, AccuVac Vial, DR/700 ....1 ..each ..46025-00 Beaker, 50 mL .
Page 362 Sample Cell, 25-ml with screw cap..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 52-36...
Page 363 Digestion (Section I). Note: The DR/700 must be calibrated before sample measurement. See Calibration following these steps. *Adapted from Standard Methods for the Examination of Water and Wastewater.
Page 364 CADMIUM, continued Fill a 250-mL Add the contents Add 30 mL of graduated cylinder to of one Buffer Powder chloroform to a the 250-mL mark Pillow, citrate type 50-mL graduated with sample. Pour for heavy metals. mixing cylinder. Add the sample into a Stopper and shake to the contents of one 500-mL separatory...
Page 365 CADMIUM, continued 15 seconds 60 seconds 1 minute Add 20 mL of Add 30 mL of Let the funnel 50% Sodium DithiVer Solution to stand undisturbed for Hydroxide Solution the 500-mL about 1 minute. and the a 0.1-g scoop separatory funnel. of potassium cyanide Stopper, invert, and Note: The bottom layer...
Page 366 3. cell (the prepared sample). Cap. Note: Typical indoor lighting permits the Note: The cadmium- DR/700 to operate with dithizone complex is the cell compartment stable for hours if the open. In bright sample cell is kept sunlight, it may be...
Page 367 0 mg/L and the show the results in lighting permits the zero and S1 prompts µg/L cadmium (Cd). DR/700 to operate with will turn off. the cell compartment Note: For best results, a open. In bright reagent blank should be...
Page 368 If these increases do not occur, see Standard Additions (Section I) for more information. CALIBRATION The DR/700 must be calibrated in order to use the Cadmium Dithizone test procedure. To obtain most accurate results, perform a new calibration when a new lot of DithiVer Metals Reagent Powder Pillows is used.
Page 369 CADMIUM, continued h) For Standard 1, use the blank from the cadmium procedure and make the display show 0000 µg/L. i) For Standard 2, use the prepared sample from the cadmium procedure and make the display show 0080 µg/L. INTERFERENCES The following do not interfere: Aluminum Lead...
Page 370 CADMIUM, continued c) Extract the solution with several 2 or 3 mL portions of pure chloroform to remove any remaining DithiVer, again discarding the bottom layer each time. d) Continue with Step 7. e) In Step 8, substitute 28.5 mL of DithiVer solution for the 30 mL. f) Continue with Step 9.
Page 371 50 mL ..1 ..each ... 1896-41 DR/700 Module Number 52.01... 1 ..each ..46252-00 Flask, volumetric, 100 mL .
Page 372 Tongs, crucible ..... . . each ... . 569-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 52-46...
Page 373 USEPA accepted for reporting** USING POWDER PILLOWS Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm concentration units, Note: Samples must be and module number and the zero prompt.
Page 374 CHLORINE, FREE, continued Fill a 10-mL cell Add the contents Immediately to the 10-mL line of one DPD Free (within 1 minute of with sample. Chlorine Powder reagent addition) Pillow to the cell (the place the prepared prepared sample). sample into the cell Cap the cell and holder.
Page 375 CHLORINE, FREE, continued Press: READ The display will count down to 0. Then the display will show the results in mg/L free chlorine Note: If the sample temporarily turns yellow after reagent addition, or flashes the upper range limit, dilute a fresh sample and repeat the test.
Page 376 USING ACCUVAC AMPULS Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm concentration units, Note: Samples must be and module number and the zero prompt.
Page 377 CHLORINE, FREE, continued Insert the Fill a DPD Free Quickly invert the AccuVac Vial Chlorine Reagent ampul several times Adapter into the cell AccuVac Ampul with to mix. Wipe off any holder. sample. liquid or fingerprints. Note: Keep the tip Note: A pink color will immersed while the develop if free chlorine...
Page 378 CHLORINE, FREE, continued Immediately Press: READ (within 1 minute of sample addition) The display will place the AccuVac count down to 0. ampul into the cell Then the display will holder. show the results in mg/L free chlorine Note: In bright light it may be necessary to Note: If the sample close the cell...
Page 379 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 1.00 mg/L Cl concentration solutions, the standard deviation was ±0.007 mg/L Cl Testing zero concentration samples, the limit of detection was 0.005 mg/L...
Page 380 ..1 ..each ... . 968-00 DR/700 Filter Module Number 52.01... 1 ..each ..46252-00 REQUIRED APPARATUS (Using AccuVac Ampuls) Adapter, AccuVac vial.
Page 381 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 52-55...
Page 382 52-56...
Page 383 USEPA accepted for reporting** USING POWDER PILLOWS Install module Press: After 2 seconds 52.01 the display will show in a DR/700. The display will show a program number, 525 nm concentration units Note: Samples must be and module number and the zero prompt.
Page 384 CHLORINE, TOTAL, continued 3 minutes Fill a 10-mL cell Add the contents Wait 3 minutes. to the 10-mL line of one DPD Total with sample. Chlorine Powder Pillow to the sample Note: A 25-mL sample cell (the prepared sample). Cap the can be tested by using sample cell and shake 25-mL sample cells and...
Page 385 CHLORINE, TOTAL, continued Fill a 10-mL cell Place the blank in Press: ZERO to the 10-mL line the cell holder. with sample (the The display will blank). Cap. Note: In bright light it count down to 0. Then the display will may be necessary to show 0.00 mg/L and close the cell...
Page 386 CHLORINE, TOTAL, continued USING ACCUVAC AMPULS Install the DR/700 Press: After 2 seconds module number the display will show 52.01 The display will show a program number, in the DR/700. 525 nm concentration units and module number and the zero prompt.
Page 387 CHLORINE, TOTAL, continued 3 minutes Wait 3 minutes. Place the blank in Press: ZERO the cell holder. The display will count down to 0. Note: In bright light it Then the display will show 0.00 mg/L and may be necessary to the zero prompt will close the cell turn off.
Page 388 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 1.00 mg/L Cl concentration solutions, the standard deviation was ±0.005 mg/L Cl Testing zero concentration samples, the limit of detection was 0.010 mg/L...
Page 389 ..1 ..each ... . 968-00 DR/700 Filter Module Number 52.01... 1 ..each ..46252-00...
Page 390 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 52-64...
Page 391 Method 8030 HARDNESS (0 to 2.00 mg/L Mg as CaCO ; 0 to 2.7 mg/L Ca as CaCO For water, wastewater, seawater Calcium and Magnesium; Calmagite Colorimetric Method Pour 100 mL of Add 1.0 mL of Add 1.0 mL of water sample into a Calcium and Alkali Solution for...
Page 392 Install module Press: After 2 seconds, number the display will show 52.01 The display will show a program number, in a DR/700. 525 nm the concentration and module number units, decimal 52.01 position and the zero prompt. If necessary, press the...
Page 393 0. Then the display will Note: Typical indoor lighting permits the show 0.00 mg/L and lighting permits the DR/700 to operate with the zero prompt will DR/700 to operate with the cell compartment turn off. the cell compartment cover open.
Page 394 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.23 mg/L Mg as CaCO and 1.875 mg/L Ca as CaCO concentration samples, the standard deviation was ±0.020 mg/L as CaCO...
Page 395 HARDNESS, continued INTERFERENCES For the most accurate calcium test result, the test should be rerun on a diluted sample if the calcium is over 1.0 and the magnesium is over 0.25 mg/L as CaCO . No retesting is needed if either is below those respective concentrations.
Page 396 Thermometer, -20 to 105 °C ... . . each ... 1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach Office or distributor serving you. 52-70...
Page 397 DPD Method* (Powder Pillows or AccuVac Ampuls) USING POWDER PILLOWS Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm the concentration Note: Samples must be and module number units, decimal 52.01...
Page 398 IODINE, continued 3 minutes Fill a 10-mL cell Add the contents Wait 3 minutes. to the 10-mL line of one DPD Total with sample. Chlorine Powder Pillow to the sample Note: A 25-mL sample cell (the prepared sample). Cap and can be tested by using shake the cell for 20 25-mL sample cells and...
Page 399 IODINE, continued Within 3 Press: READ minutes after the 3- minute period, place The display will the prepared sample count down to 0. in the cell holder. Then the display will show the results in Note: In bright sunlight mg/L iodine (I it may be necessary to close the cell compartment cover.
Page 400 IODINE, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds, 52.01 the display will show in a DR/700. The display will show a program number, 525 nm the concentration Note: Sample must be and module number units, decimal 52.01...
Page 401 IODINE, continued 3 minutes Wait 3 minutes. Place the blank in Press: ZERO the cell holder. The display will Note: In bright sunlight count down to 0. Then the display will it may be necessary to show 0.00 mg/L and close the cell the zero prompt will compartment cover.
Page 402 If these increases do not occur, see Standard Additions (Section I) for more information. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.00 mg/L Cl (equivalent to 3.58 mg/L I ) concentration samples, the standard deviation was ±0.005 mg/L Cl...
Page 403 ..1 ..each ... 968-00 DR/700 Filter Module Number 52.01... 1 ..each ..46252-00 REQUIRED APPARATUS (Using AccuVac Ampuls) Beaker, 50 mL .
Page 404 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Ampule Breaker Kit .
Page 405 Storage following these and is hazardous to skin steps. Adjust the pH of and other materials. the stored samples before analysis. Note: The DR/700 must be calibrated before sample measurement. See Calibration following these steps. Note: Clean all glassware with a Nitric Acid Solution, 1:1.
Page 406 LEAD, continued Add 50 mL of Add 30 mL of the Continue adding chloroform to a dithizone solution to 5.0 N Sodium graduated mixing the funnel. Stopper, Hydroxide Standard cylinder. Add the invert, and open the Solution, dropwise, contents of one stopcock slowly to until the color of the DithiVer Metals...
Page 407 LEAD, continued Add 2 heaping Insert a pea-sized Fill a 25-mL cell 1.0 g scoops of cotton plug into the to the 25-mL line potassium cyanide, delivery tube of the with chloroform (the ACS, to the funnel. funnel. Drain the blank).
Page 408 LEAD, continued Install module Press: After 2 seconds, 52.01 the display will show a in a DR/700 The display will show program number, the 525 nm concentration units, and module decimal position and 52.01 the zero prompt. Press once of...
Page 409 Note: Typical indoor lighting permits the 0 µg/L and the zero lighting permits the DR/700 to operate with prompt will turn off. DR/700 to operate with the cell compartment the cell compartment cover open. In bright cover open.
Page 410 LEAD, continued Press: READ The display will count down to 0. Then the display will show the results in µg/L lead (Pb). Note: For best results, a reagent blank should be determined for each new lot of DithiVer Metals Reagent Powder Pillows.
Page 411 If these increases do not occur, see Standard Additions (Section I) for more information. CALIBRATION The DR/700 must be calibrated in order to use the Lead Dithizone test procedure. To obtain the most accurate results, a new calibration should be done when a different manufacturing lot of DithiVer Metals Reagent Powder Pillows is used.
Page 412 LEAD, continued INTERFERENCES The following do not interfere. Aluminum Cobalt Antimony Iron Arsenic Magnesium Cadmium Manganese Calcium Nickel Chromium Zinc The following interfere. Bismuth Silver Copper Mercury Eliminate interference from these metals by the following treatment, beginning after procedure Step 4. a) Measure about 5 mL of the prepared DithiVer solution into the separatory funnel.
Page 413 LEAD, continued Highly buffered samples or extreme sample pH may exceed the buffering capacity of the reagents and require sample pretreatment; see Interferences, pH (Section I). WASTE DISPOSAL Disposal of cyanide-containing wastes by following the steps below. a) Use good ventilation or a fume hood b) Add the waste, while stirring, to a beaker containing a strong solution of sodium hydroxide and calcium hypochlorite or sodium hypochlorite (household bleach).
Page 414 Cylinder, graduated, 250 mL . . 1 ..each ... . 508-46 DR/700 Filter Module Number 52.01... 1 ..each ..46252-00 Flask, volumetric, 100 mL .
Page 415 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach Office or distributor serving you. 52-89...
Page 416 52-90...
Page 417 Install module Press: After 2 seconds, number the display will show 52.01 The display will show a program number, in a DR/700. 525 nm the concentration and module number units, decimal Note: If samples cannot 52.01 position and the zero prompt.
Page 418 MANGANESE, HR, continued Fill a 10-mL cell Add the contents Add the contents to the 10 mL line with of one Buffer Powder of one Sodium sample. Pillow, citrate type. Periodate Powder Swirl to mix. Pillow to the sample Note: A 25-mL sample cell (the prepared Note: For proof of sample).
Page 419 MANGANESE, HR, continued 8 minutes Press: Within eight Press: ZERO READ minutes after the 2- The display will minute period, place The display will count count down to 0. the prepared sample down to 0. Then the Then the display will in the cell holder.
Page 420 MANGANESE, HR, continued SAMPLING AND STORAGE Collect samples in acid-washed plastic bottles. Manganese may be lost by adsorption to glass container walls. Adjust the pH to 2 or less with nitric acid (about 2 mL per liter). Preserved samples may be stored at room temperature for 6 months.
Page 421 Interferences, pH (Section I). STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 10.0 mg/L Mn concentration samples, the standard deviation was ±0.11 mg/L Mn.
Page 422 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 52-96...
Page 423 Install module Press: After 2 seconds, number the display will show 52.01 The display will show a program number, in a DR/700. 525 nm the concentration and module number units, decimal Note: Samples must be 52.01 position and the zero prompt.
Page 424 OXYGEN, DISSOLVED, HR, continued 30 seconds Fill a blue ampul Fill a High Range Without inverting cap with sample. Fill Dissolved Oxygen the ampul, a 10-mL cell with AccuVac Ampul with immediately place the 10 mL of sample. sample (the prepared ampul cap that was Collect at least 40 mL sample).
Page 425 OXYGEN, DISSOLVED, HR, continued Press: Insert the Wipe dry and ZERO AccuVac Vial place the prepared The display will Adapter into the cell sample in the cell count down to 0. holder. holder. Then the display will show 0.0 mg/L and Note: In bright sunlight the zero prompt will it may be necessary to...
Page 426 STATISTICAL EVALUATION A single operator repetitively tested samples of the same solutions, using one DR/700 and two representative lots of AccuVac Ampuls. Testing 5 concentration samples, the standard deviation was ±0.23 mg/L mg/L O Testing zero concentration samples, the limit of detection was 0.11 mg/L...
Page 427 Dissolved oxygen may also be determined by titrimetric methods. Request Publication 1171 for additional information. For Technical Assistance Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 52-101...
Page 428 52-102...
Page 429 Method 8180 PHOSPHORUS, ACID HYDROLYZABLE For water, wastewater, seawater Hydrolysis to Orthophosphate Method* 30 minutes Measure 25 mL of Add 2.0 mL of Place the flask sample into a 50-mL Sulfuric Acid (the prepared sample) erlenmeyer flask Solution, 5.25 N. on a hot plate.
Page 430 PHOSPHORUS, ACID HYDROLYZABLE, continued Cool the prepared Add 2.0 mL of Pour the prepared sample to room 5.0 N Sodium sample into a temperature. Hydroxide Solution. graduated cylinder. Swirl to mix. Add demineralized water rinsings from Note: Use the 1-mL the flask to return the volume to 25 mL.
Page 431 PHOSPHORUS, ACID HYDROLYZABLE continued SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed immediately. If prompt analysis is not possible, samples may be preserved up to 24 hours by cooling to 4 °C (39 °F). Warm to room temperature before testing.
Page 432 Meter, EC10, portable ....each ..50050-00 For Technical Information Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. *Contact Hach for larger sizes. 52-106...
Page 433 Install module Press: After 2 seconds, number the display will show 52.01 The display will show a program number, in a DR/700. 525 nm the concentration and module number units, decimal Note: If samples cannot 52.01 position and the zero prompt.
Page 434 PHOSPHORUS REACTIVE, continued Add 1 mL of Add 1 mL of Fill a 25-mL Molybdate Reagent Amino Acid Reagent graduate mixing using a 1-mL Solution. Stopper cylinder with 25 mL calibrated dropper. and invert several of sample. times to mix (the prepared sample).
Page 435 PHOSPHORUS REACTIVE, continued Press: Fill a 10-mL cell Place the ZERO to the 10-mL line prepared sample in The display will with prepared the cell holder. count down to 0. sample. Then the display will Note: In bright sunlight, show 0.00 mg/L and it may be necessary to the zero prompt will close the cell...
Page 436 PHOSPHORUS REACTIVE, continued SAMPLING AND STORAGE Collect samples in plastic or glass bottles that have been acid cleaned with 1:1 Hydrochloric Acid Solution and rinsed with demineralized water. Do not use commercial detergents containing phosphate for cleaning glassware used in this test. Analyze samples immediately after collection for best results.
Page 437 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 15.0 mg/L PO concentration samples, the standard deviation was ±0.10 mg/L PO Testing zero concentration samples, the limit of detection was 0.23 mg/L...
Page 438 ..1 ..each ..1896-40 DR/700 Filter Module Number 52.01... 1 ..each ..46252-00 OPTIONAL REAGENTS Amino Acid Reagent Powder Pillow .
Page 439 Thermometer, -20 to 105 °C ... . . each ... 1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach Office or distributor serving you. *Contact Hach for larger sizes. 52-113...
Page 440 52-114...
Page 441 Method 8190 PHOSPHORUS, TOTAL For water, wastewater and seawater (also called Organic and Acid Hydrolyzable) Acid Persulfate Digestion Method*; USEPA accepted for reporting Measure 25 mL Add the contents Add 2.0 mL of of sample into a of one Potassium 5.25 N Sulfuric Acid 50-mL erlenmeyer Persulfate Powder...
Page 442 PHOSPHORUS, TOTAL, continued 30 minutes Place the flask on Cool the sample Add 2.0 mL of a hot plate. Boil to room temperature. 5.0 N Sodium gently for 30 minutes. Hydroxide Solution. Swirl to mix. Note: Maintain the Note: Use the 1-mL volume near 20 mL by calibrated dropper adding small amounts of...
Page 443 PHOSPHORUS, TOTAL, continued Pour the sample into a 25-mL graduated cylinder. Using demineralized water rinsings from the flask, return the volume in the cylinder to 25 mL. Proceed with a reactive phosphorus test of the expected total phosphorus concentration range. Note: Results of the reactive phosphorus test at this point will include...
Page 444 PHOSPHORUS, TOTAL, continued SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed immediately. If prompt analysis is not possible, samples may be preserved up to 24 hours by cooling to 4 °C (39 °F). Warm to room temperature before testing.
Page 445 Meter, EC10, portable ....each ..50050-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A - Contact the Hach office or distributor serving you. 52-119...
Page 446 52-120...
Page 447 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 448 Table of Contents for 550-nm parameters Chromium, Hexavalent, Sample Cell and AccuVac Ampul..55-1 Chromium, Total ........55-11 Copper, Bicinchoninate, Sample Cell and AccuVac Ampul .
Page 449 AccuVac Ampuls), USEPA accepted for reporting** USING POWDER PILLOWS Install module Press: After 2 seconds, 55.01 the display will show in a DR/700. The display will show a program number, 550 nm the concentration and module number units and the zero 55.01...
Page 450 CHROMIUM, HEXAVALENT, continued 5 minutes Fill a 10-mL cell Add the contents Wait 5 minutes. to the 10-mL line of one ChromaVer 3 with sample. Reagent Powder Pillow to the cell (the Note: A 25-mL sample prepared sample). Cap. Invert several can be tested by using times to mix.
Page 451 CHROMIUM, HEXAVALENT, continued Fill a 10-mL cell Place the blank in Press: ZERO to the 10-mL line the cell holder. with the sample (the The display will Note: In bright light it blank). Cap. count down to 0. Then the display will may be necessary to Note: For turbid show 0.000 mg/L and...
Page 452 CHROMIUM, HEXAVALENT, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Then the Note: In bright light it display will show the results in mg/L may be necessary to hexavalent chromium. close the cell compartment cover.
Page 453 CHROMIUM, HEXAVALENT, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds, 55.01 the display will show in a DR/700. The display will show a program number, 550 nm concentration units and module number and the zero prompt. 55.01 If necessary, press the...
Page 454 CHROMIUM, HEXAVALENT, continued Fill a cell with Fill a ChromaVer Quickly invert the 10 mL of sample (the 3 Reagent AccuVac ampul several times blank). Cap. Collect Ampul with sample to mix. Wipe off any at least 40 mL of (this is the prepared liquid or fingerprints.
Page 455 CHROMIUM, HEXAVALENT, continued 5 minutes Wait 5 minutes. Place the blank in Press: ZERO the cell holder. The display will Note: In bright light it count down to 0. Then the display will may be necessary to show 0.000 mg/L and close the cell the zero prompt will compartment cover.
Page 456 0.25 mg/L Cr STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 4.00 mg/L Cr concentration solutions, the standard deviation was ±0.0026 mg/L Cr Testing zero concentration samples, the limit of detection was 0.0132 mg/L Cr...
Page 457 ..1 ..each ... . 968-00 DR/700 Filter Module Number 55.01... 1 ..each ..46255-00...
Page 458 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 55-10...
Page 459 Method 8024 CHROMIUM, TOTAL (0 to 0.700 mg/L) For water and wastewater Alkaline Hypobromite Oxidation Method* Fill a clean 25-mL Add the contents Place the prepared mixing bottle with of one Chromium 1 sample into a boiling 25 mL of sample. Reagent Powder water bath.
Page 460 Undissolved powder does not affect accuracy. 5 minutes Wait 5 minutes. During the 5 Press: minute period, install module The display will show 55.01 550 nm in a DR/700. and module number 55.01 55-12...
Page 461 Note: Typical indoor press the UP ARROW samples, treat the blank lighting permits the key until the lower as described in Steps 1 DR/700 to operate with display shows through 8. the cell compartment program number open. In bright 55.03.1...
Page 462 Then the display will show 0.000 mg/L and show the results in lighting permits the the zero prompt will mg/L chromium. DR/700 to operate with turn off. Subtract the the cell compartment ChromaVer 3 open. In bright Reagent blank value...
Page 463 Prepare this solution daily. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 0.400 mg/L Cr concentration solutions, the standard deviation was ±0.0024 mg/L Cr.
Page 464 ..1 ..each ... 968-00 DR/700 Filter Module Number 55.01... 1 ..each ..46255-00 Water bath and rack .
Page 465 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 55-17...
Page 466 55-18...
Page 467 USEPA Approved for reporting** (digestion required; see Section 1)† USING POWDER PILLOWS Install module Press: After 2 seconds, 55.01 the display will show in a DR/700. The display will show a program number, 550 nm the concentration Note: If samples cannot and module number units and the zero 55.01...
Page 468 COPPER, continued 2 minutes Fill a 10-mL Add the contents Wait 2 minutes. sample cell to the of one CuVer 1 10-mL line with Copper Reagent sample. Powder Pillow to the sample cell (the Note: A 25-mL sample prepared sample). Cap and invert several can be tested by using times to mix.
Page 469 COPPER, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Note: In bright light it Then the display will show the results in may be necessary to mg/L copper (Cu). close the cell compartment cover.
Page 470 USING ACCUVAC AMPULS Install module Press: After 2 seconds, 55.01 the display will show in a DR/700. The display will show a program number, 550 nm concentration units Note: If samples cannot and module number and the zero prompt.
Page 471 COPPER, continued 2 minutes Wait 2 minutes. Place the blank in Press: ZERO the cell holder. The display will Note: In bright light it count down to 0. Then the display will may be necessary to show 0.00 mg/L and close the cell the zero prompt will compartment cover.
Page 472 Prepare this solution daily. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 2.50 mg/L Cu concentration solutions, the standard deviation was ±0.012 mg/L Cu.
Page 473 COPPER, continued Using two representative lots of AccuVacs, the standard deviation was ±0.011 mg/L Cu. and the limit of detection was 0.022 mg/L Cu. INTERFERENCES (Using Powder Pillows) If the sample is extremely acidic (pH 2 or less) a precipitate may form. Add 8 N Potassium Hydroxide Standard Solution drop-wise while swirling to dissolve the turbidity.
Page 474 ..1 ..each ... . 968-00 DR/700 Filter Module Number 55.01... 1 ..each ..46255-00 REQUIRED APPARATUS (Using AccuVac Ampuls) Adapter, AccuVac vial .
Page 475 Sample Cell, 10-mL with screw cap ..6/pkg ..24276-06 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 *Contact Hach for larger sizes. 55-27...
Page 476 55-28...
Page 477 Press: After 2 seconds, number the display will show 55.01 The display will show a program number, in a DR/700. 550 nm concentration units and module number and the zero prompt. Note: Samples must be 55.01 If necessary, press the...
Page 478 DEHA, continued Fill a 25-mL cell Fill a second Add the contents to the 25-mL line sample cell with of one DEHA with sample. 25 mL of Reagent 1 Powder demineralized water Pillow to each sample Note: Rinse glassware (the blank). cell.
Page 479 Note: Typical indoor reaction time affect the Immediately place lighting permits the results. Be sure these the sample cells in the DR/700 to operate with factors are controlled as dark. the cell compartment described. open. In bright Note: A purple color...
Page 480 Note: Typical indoor show the results in zero prompt will turn µg/L DEHA. lighting permits the off. DR/700 to operate with Note: Repeat the above the cell compartment procedure, omitting Step open. In bright 7, to determine the sunlight, it may be...
Page 481 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 200 µg/L DEHA concentration solutions, the standard deviation was ±2.4 µg/L DEHA. Testing zero concentration samples, the limit of detection was 3.1 µg/L DEHA.
Page 482 Thermometer, -20 to 105 °C ... . . each ... 1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 55-34...
Page 483 For water and seawater FerroZine Method* Install module Press: After 2 seconds, 55.01 the display will show in a DR/700. The display will show a program number, 550 nm concentration units, Note: If samples cannot and module number decimal position and 55.01...
Page 484 IRON, continued 5 minutes Fill a 25-mL cell Add the contents Wait 5 minutes. to the 25-mL line of one FerroZine Iron with sample. Reagent Solution Note: A violet color will Pillow to the cell (the develop if iron is Note: Rinse glassware prepared sample).
Page 485 Note: Typical indoor blank). Cap. count down to 0. Then the display will lighting permits the show 0.000 mg/L and DR/700 to operate with the zero prompt will the cell compartment turn off. cover open. In bright sunlight it may be necessary to close the cell compartment cover.
Page 486 0. Note: Typical indoor Then the display will show the results in lighting permits the mg/L iron (Fe). DR/700 to operate with the cell compartment cover open. In bright sunlight it may be necessary to close the cell compartment cover.
Page 487 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.500 mg/L Fe concentration samples, the standard deviation was ±0.0024 mg/L Fe.
Page 488 IRON, continued INTERFERENCES Copper and cobalt may interfere to give slightly high results. EDTA interferes, use either the TPTZ or FerroVer method. The TPTZ method is suggested for low concentrations. Any of the three digestions give in Digestion (Section I) used in place of the treatments given below will eliminate the following interferences.
Page 489 ..1 ..each ... 968-00 DR/700 Filter Module Number 55.01... 1 ..each ..46255-00 OPTIONAL REAGENTS Ammonium Hydroxide, ACS .
Page 490 Thermometer, -20 to 105 °C ... . . each ... 1877-01 For Technical Assistance Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free to place an order. Outside the U.S.A. - Contact the Hach office or distributor serving you. 55-42...
Page 491 For water and wastewater PAN Method* (Digestion required) Install module Press: After 2 seconds, 55.01 the display will show in a DR/700. The display will show a program number, 550 nm concentration units Note: If samples cannot and module and the zero prompt.
Page 492 MANGANESE, LR, continued Pour 25 mL of Pour 25 mL of Add the contents demineralized water sample into another of one Ascorbic Acid into a sample cell (the sample cell (the Powder Pillow to each blank). prepared sample). cell. Cap and invert several times to mix.
Page 493 MANGANESE, LR, continued 2 minutes Add 1.0 mL of Add 1.0 mL of Wait 2 minutes. Alkaline-Cyanide 0.1% PAN Indicator Reagent Solution to Solution to each Note: If sample contains each cell. Swirl to sample cell. Cap and high amounts of iron mix.
Page 494 0. Then the display will Note: Typical indoor lighting permits the show 0.00 mg/L and lighting permits the DR/700 to operate with the zero prompt will DR/700 to operate with the cell compartment turn off. the cell compartment cover open.
Page 495 MANGANESE, LR, continued Press: READ Table 1. Conversion Factors To convert reading from Multiply by The display will mg/L Mn mg/L MnO 2.16 count down to 0. mg/L Mn mg/L KMnO 2.88 Then the display will show the results in mg/L manganese.
Page 496 Step 12 should be 0.5 mg/L Mn. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.500 mg/L Mn concentration samples, the standard deviation was ±0.0029 mg/L Mn.
Page 497 MANGANESE, LR, continued Magnesium 300 mg/L as CaCO Nickel 40 mg/L Zinc 15 mg/L WASTE DISPOSAL Dispose of all spent cyanide-containing wastes by following the steps below. a) Pour the waste into a large beaker and make alkaline (pH 11) with calcium hypochlorite or sodium hypochlorite (bleach).
Page 498 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A - Call 800-227-4224 toll-free for more information. Outside the U. S. A. - Contact the Hach office or distributor serving you. 55-50...
Page 499 Method 8150 NICKEL (0 to 1.000 mg/L) For water and wastewater 1-(2-Pyridylazo)-2-Naphthol (PAN) Method* Measure 25 mL Measure 25 mL of Add the contents of sample in a mixing demineralized water of one Phthalate- graduated cylinder. in a second cylinder Phosphate Reagent This will be the (the blank).
Page 500 Wait 15 minutes. Install module PAN Indicator Steps 6 through 8 can 55.01 Solution, 0.3%, to be performed during in a DR/700. each cylinder. this waiting period. Stopper. Invert several times to mix. Note: During color development, the Note: Use the plastic sample solution color dropper provided.
Page 501 NICKEL, continued Fill a 10 mL Place the blank Press: ZERO cell to the 10-mL line in the cell holder. with the blank The display will solution. Note: In bright sunlight count down to 0. Then the display will it may be necessary to show 0.000 mg/L and close the cell the zero prompt will...
Page 502 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.600 mg/L Ni concentration samples, the standard deviation was ±0.0043 mg/L Ni.
Page 503 NICKEL, continued 20 mg/L 40 mg/L 15 mg/L 20 mg/L 10 mg/L and CO interferes directly and must not be present. 500 mg/L 400 mg/L 25 mg/L 60 mg/L 5000 mg/L 20 mg/L 30 mg/L Highly buffered samples or extreme sample pH may exceed the buffering capacity of the reagents and required sample pretreatment;...
Page 504 Sample Cell, 25-mL, with screw cap ..6/pkg ..24019-06 *100 Tests equals 50 sample and 50 blanks. Contact Hach for larger sizes. For Technical Assistance, Prices and Ordering In the U.S.A.
Page 505 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 506 Table of Contents for 575-nm parameters Fluoride, Sample Cell and AccuVac Ampul ....57-1 Iron, Total, TPTZ ........57-11 Nickel, Autocatalytic.
Page 507 (distillation is required; see Section 1)** USING SPADNS REAGENT SOLUTION Install module Press: After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm concentration units Note: If samples cannot and the module and the zero prompt.
Page 508 The display will Note: Typical indoor count down to 0. lighting permits the Then the display will DR/700 to operate with show 0.0 mg/L and the cell compartment the zero prompt will open. In bright sunlight, turn off.
Page 509 0. Note: Typical indoor Then the display will show the results in lighting permits the mg/L fluoride (F DR/700 to operate with the cell compartment Note: If the instrument open. In bright flashes the upper range sunlight, it may be...
Page 510 USING ACCUVAC AMPULS Install module Press After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm concentration units Note: If samples cannot and module number and the zero prompt.
Page 511 FLUORIDE, continued 1 minute Wait 1 minute. Insert the Place the blank in AccuVac Vial the cell holder. Adapter into the cell Note: In bright light, it holder. may be necessary to close the cell compartment cover. Press: Place the Press: ZERO READ...
Page 512 Standard Solution Method A variety of standard solutions covering the entire range of the test is available from Hach. Use these in place of sample to verify technique. Minor variations between lots of reagent become measurable above 1.5 mg/L. While results in this region are usable for most purposes, better accuracy may be obtained by diluting a fresh sample 1:1 with demineralized water and retesting.
Page 513 Set up the distillation apparatus for the general purpose distillation. See the Hach Distillation Apparatus Manual. Turn on the water and make certain it is flowing through the condenser. b) Measure 100 mL of sample into the distillation flask. Add a magnetic stirring bar and turn on the heater power switch.
Page 514 Cylinder, graduated, 25 mL . . 1 ..each ... . 508-40 DR/700 Filter Module Number 57.01... 1 ..each ..46257-00 Pipet Filler, safety bulb .
Page 515 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Cap for 10- and 25-mL sample cells .
Page 516 57-10...
Page 517 TPTZ Method* (Powder Pillows or AccuVac Ampuls) USING POWDER PILLOWS Install module Press: After 2 seconds 57.01 the display will show in a DR/700. The display will show a program number, 575 nm concentration units, Note: If samples cannot and module the decimal position 57.01...
Page 518 IRON, TOTAL, continued 3 minutes Fill a 25-mL cell to Add the contents Wait 3 minutes. the 25-mL line with of one TPTZ Iron sample. Reagent Powder Note: Continue with Pillow (the prepared steps 7 and 8 while the Note: For proof of sample).
Page 519 Note: Typical indoor water. Pillow to the demineralized water lighting permits the (the blank). Cap. DR/700 to operate with Shake for 30 seconds. the cell compartment cover open. In bright sunlight, it might be necessary to close the cell compartment cover.
Page 520 L Iron (Fe). turn off. Note: Typical indoor lighting permits the DR/700 to operate with the cell compartment cover open. In bright sunlight, it might be necessary to close the cell compartment cover.
Page 521 IRON, TOTAL, continued USING ACCUVAC AMPULS Install module Press: After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm concentration units, Note: If sample cannot and module number decimal position and 57.01...
Page 522 IRON, TOTAL, continued Fill a cell with 10 Fill a TPTZ Iron Invert the ampul mL of sample (the AccuVac Ampul with (the prepared sample) blank). Cap. Collect sample. repeatedly to mix. at least 40 mL of Wipe off any liquid or sample in a 50 mL Note: Keep tip fingerprints.
Page 523 IRON, TOTAL, continued Insert the Within 30 Press: READ AccuVac Vial minutes after the 3- Adapter into the cell minute waiting The display will holder. period, place the count down to 0. prepared sample in Then the display will the cell holder. show the results in mg/L iron (Fe).
Page 524 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.75 mg/L Fe concentration samples, the standard deviation was ±0.035 mg/L Fe.
Page 525 IRON, TOTAL, continued INTERFERENCES In the powder pillow procedure if the sample, (without TPTZ Iron Reagent) has a color or turbidity greater than the blank of Step 8 (demineralized water plus TPTZ Iron Reagent), use the sample as the blank. A sample pH of less than 3 or greater than 4 after the addition of reagent may inhibit color formation, cause the developed color to fade quickly or result in turbidity.
Page 526 ..1 ..each ... 968-00 DR/700 Filter Module Number 57.01... 1 ..each ..46257-00 REQUIRED APPARATUS (Using AccuVac Ampuls) Beaker, 50 mL .
Page 527 Sample Cell, 25-mL with screw cap ..each ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 57-21...
Page 528 57-22...
Page 529 (0 to 7.00 g/L) For finishing baths Photometric Method Install module Press: After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm the concentration and module number units, decimal 57.01 position and the zero prompt.
Page 530 NICKEL, AUTOCATALYTIC, continued Place the blank in Press: Place the prepared ZERO the cell holder. sample in the cell The display will holder. Note: In bright sunlight count down to 0. Then the display will Note: In bright sunlight it may be necessary to show 0.00 g/L and the it may be necessary to close the cell...
Page 531 Step 5. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 2.19 g/L Ni concentration samples the standard deviation was ±0.019 g/L Ni.
Page 532 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 57-26...
Page 533 For water and wastewater Ferrous Sulfate Method* Install module Press: After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm concentration units, Note: If sample cannot and module decimal position and 57.01...
Page 534 NITRITE, HR, continued 10 minutes Fill a 10-mL Add the contents Wait 10 minutes. sample cell to the of one NitriVer 2 10-mL line with Nitrite Reagent sample. Powder Pillow. Cap and shake to dissolve Note: A 25-mL sample (the prepared sample).
Page 535 NITRITE, HR, continued Invert the Press: READ prepared sample twice. Then place the The display will prepared sample in count down to 0. the cell holder. Then the display will show the results in Note: In bright sunlight mg/L nitrite. it may be necessary to Note: To convert results close the cell...
Page 536 Prepare this solution daily. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells, and two representative lots of testing reagents. Testing 79.8 mg/L NO concentration samples, the standard deviation was ±3.8 mg/L NO Testing zero concentration samples, the limit of detection was 3.9 mg/L...
Page 537 Cap, for 10-mL and 25-mL sample cells..12/pkg ..24018-12 DR/700 Filter Module Number 50.01 ..each ..46250-00 Flask, volumetric, 1000 mL .
Page 538 57-32...
Page 539 For water, wastewater, cooling tower and pool/spa water Direct Binary Complex Method Install module Press: After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm concentration units and module number and the zero prompt.
Page 540 The display will Note: Typical indoor count down to 0. Then the display will lighting permits the show 0.0 mg/L and DR/700 to operate with the zero prompt will the cell compartment turn off. cover open. In bright sunlight it may be necessary to close the cell compartment cover.
Page 541 0. Note: Typical indoor Then the display will show the results in lighting permits the mg/L quaternary DR/700 to operate with ammonium the cell compartment compounds as CTAB. cover open. In bright sunlight it may be necessary to close the cell compartment cover.
Page 542 QUATERNARY AMMONIUM COMPOUNDS, continued c) Analyze 25 mL of the 5 mg/L CTAB standard solution according to the preceding procedure. The result should be 5.0 ±0.1 mg/L. Standard Additions Method a) Use a TenSette Pipet to add 0.5, 1.0 and 1.5 mL of Q.A.C. Standard Solution, 100 mg/L as CTAB, to three 50-mL samples.
Page 543 STATISTICAL EVALUATION A single operator repetitively tested samples of two prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 2.8 mg/L CTAB concentration samples, the standard deviation was ±0.12 mg/L CTAB.
Page 544 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 57-38...
Page 545 For water and wastewater Colorimetric Method Install module Press: After 2 seconds, 57.01 the display will show in a DR/700. The display will show a program number, 575 nm the concentration Note: If sample cannot and module number units, decimal 57.01...
Page 546 SILVER, continued Add the contents Add the contents Using a 50-mL of one Silver 1 of one Silver 2 graduated cylinder, Powder Pillow to a Reagent Pillow to the add 50-mL of sample dry 50-mL mixing cylinder. Swirl to (adjusted to pH 9-10) graduated cylinder.
Page 547 SILVER, continued Place the blank Press: ZERO Place the in the cell holder. prepared sample in The display will the cell holder. Note: In bright sunlight count down to 0. Then the display will Note: In bright sunlight it may be necessary to show 0.000 mg/L and it may be necessary to close the cell...
Page 548 SILVER, continued SAMPLING AND STORAGE Collect samples in acid-cleaned plastic or glass bottles. Using pH paper, adjust the pH to 2 or less with nitric acid (about 2 mL/liter). Store preserved samples at room temperature for up to six months. Adjust the pH to 9 to 10 with 5.0 N sodium hydroxide before analysis.
Page 549 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.500 mg/L Ag concentration samples the standard deviation was ±0.0036 mg/L Ag.
Page 550 SILVER, continued Caution: Poisonous hydrogen cyanide gas is generated during this digestion. Always wear safety glasses and use a safety shield, or operate the Digesdahl within a closed fume hood. Additional safety precautions are given in General Digesdahl Digestion (Section 1). a) Add an appropriate size sample to the 100-mL volumetric flask of the Digesdahl.
Page 551 SILVER, continued g) Add another 3 mL of sulfuric acid via the capillary funnel. Add another 5 mL of hydrogen peroxide. Check the solution for digestion completion. If digestion is not complete, continue adding hydrogen peroxide in 5 to 10 mL portions. Several portions may be necessary. Note: Digestion is complete when the digestate is colorless or the color of the digestate does not change upon addition of hydrogen peroxide.
Page 552 50 mL ..1 ..each ... 1896-41 DR/700 Filter Module Number 57.01... 1 ..each ..46257-00 OPTIONAL REAGENTS Hydrogen Peroxide, 50% .
Page 553 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 57-47...
Page 554 57-48...
Page 555 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 556 Table of Contents for 610-nm parameters Boron ..........61-1 Cobalt .
Page 557 Method 8015 BORON (0 to 14.0 mg/L) For water and wastewater Carmine Method* Measure 75.0 mL Add the contents Accurately pipet of sulfuric acid, ACS, of one BoroVer 3 2.0 mL of using a 100-mL Reagent Powder demineralized water graduated cylinder, Pillow to the flask.
Page 558 Steps 4 and 5. Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm the concentration and module number units and the zero 61.01 prompt.
Page 559 BORON, continued Pour 10 mL of Place the blank Press: ZERO the blank and 10 mL in the cell holder. of the prepared The display will sample into separate Note: In bright light, it count down to 0. sample cells. Label Then the display will may be necessary to and cap the cells...
Page 560 BORON, continued SAMPLING AND STORAGE Collect samples in polyethylene bottles or alkali-resistant boron-free glass. REAGENT PREPARATION Prepare additional BoroVer 3/sulfuric acid solution by mixing one BoroVer 3 Reagent Powder Pillow per 75 mL of sulfuric acid, ACS, adding the powder pillows individually with stirring. Preparation of this solution generates gaseous HCl when the indicator pillow is added to the concentrated sulfuric acid.
Page 561 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 8.0 mg/L B concentration solutions, the standard deviation was ±0.11 mg/L B. Testing zero concentration samples, the limit of detection was 0.44 mg/L B.
Page 562 Sample cell, 25-mL, with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 61-6...
Page 563 For water and wastewater 1-(2-Pyridylazo)-2-Naphthol (PAN) Method* Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm the concentration Note: If samples cannot and module number units and the zero 61.01...
Page 564 COBALT, continued Measure 25 mL of Measure 25 mL of Add the contents sample in a 25-mL demineralized water of one Phthalate- graduated mixing in a second 25-mL Phosphate Reagent cylinder (the prepared cylinder (the blank). Powder Pillow to sample). each cylinder.
Page 565 COBALT, continued Fill a 10-mL cell Place the blank Press: ZERO to the 10-mL line in the cell holder. with the blank. Cap. The display will Note: In bright light, it count down to 0. Then the display will may necessary to close show 0.00 mg/L and the cell compartment the zero prompt will...
Page 566 This is a 10-mg/L cobalt standard solution. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 1.0 mg/L Co concentration solutions, the standard deviation was ±0.004 mg/L Co.
Page 567 COBALT, continued 10 mg/L 500 mg/L 400 mg/L 25 mg/L 60 mg/L 5000 mg/L 20 mg/L 30 mg/L Highly buffered samples or extreme sample pH may exceed the buffering capacity of the reagents and require sample pretreatment; see Interferences, pH (Section I). SUMMARY OF METHOD After buffering the sample and masking any Fe with pyrophosphate,...
Page 568 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 61-12...
Page 569 Method 8027 CYANIDE (0 to 0.200 mg/L) For water, wastewater and seawater Pyridine-Pyrazalone Method* 30 seconds Fill a 10-mL cell Add the contents Shake the sample to the 10-mL line of one CyaniVer 3 cell for 30 seconds. with sample. Cyanide Reagent Powder Pillow.
Page 570 CYANIDE, continued 30 seconds 10 seconds Wait an additional Add the contents Shake the sample 30 seconds, leaving of one CyaniVer 4 cell for 10 seconds. the sample cell Cyanide Reagent Proceed immediately undisturbed. Powder Pillow. Cap with Step 7. the sample cell.
Page 571 CYANIDE, continued Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm the concentration and module number units and the zero 61.01 prompt. If necessary, press the...
Page 572 CYANIDE, continued Place the Press: READ prepared sample in the cell holder. The display will count down to 0. Then the display will Note: In bright light it show the results in may be necessary to mg/L cyanide (CN close the cell compartment cover.
Page 573 CYANIDE, continued or sample distillation procedures should be adjusted to approximately pH 7 with 2.5 N Hydrochloric Acid Standard Solution. When significant amounts of preservative are used, correct for volume additions; see Sampling and Storage, Volume Additions, (Section I) for more information.
Page 574 CYANIDE, continued a) Place a drop of sample on a disc of hydrogen sulfide test paper that has been wetted with pH 4 Buffer Solution. b) If the test paper darkens, add a 1-g measuring spoon of lead acetate to the sample.
Page 575 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 0.098 mg/L CN concentration solutions, the standard deviation was ±0.0028 mg/L CN Testing zero concentration samples, the limit of detection was 0.0011 mg/L CN...
Page 576 CYANIDE, continued c) Add Sodium Arsenite Solution drop-wise until the sample turns colorless. Swirl the sample thoroughly after each drop. Count the number of drops. d) Take another 25-mL sample and add the total number of drops of Hydrochloric Acid Standard Solution counted in Step a. e) Subtract one drop from the amount of Sodium Arsenite Solution added in Step c.
Page 577 CYANIDE, continued Acid Distillation For USEPA reporting purposes, samples must be distilled. All samples to be analyzed for cyanide should be treated by acid distillation except when experience has shown that there is no difference in results obtained with or without distillation. With most compounds, a one-hour reflux is adequate.
Page 578 Hach: a) Set up the distillation apparatus for cyanide recovery, leaving off the thistle tube. Refer to the Hach Distillation Apparatus Manual. Turn on the water and make certain it is flowing steadily through the condenser. b) Fill the distillation apparatus cylinder to the 50-mL mark with 0.25 N Sodium Hydroxide Standard Solution.
Page 579 ..1 ..each ... . 968-00 DR/700 Filter Module Number 61.01... 1 ..each ..46261-00...
Page 580 CYANIDE, continued OPTIONAL REAGENTS Cat. No. Cyanide Reagent Set (100 Tests) 25-mL sample size ..22428-00 Includes: (1) 14039-69, (1) 14040-99, (1) 14041-69 Description Unit Cat. No. Acetic Acid Solution, 10% ....500 mL ..14816-49 Ascorbic Acid .
Page 581 Cyanide can also be measured directly using the Cyanide/Iodide Ion selective electrode, Cat. No. 44410-71 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 61-25...
Page 582 61-26...
Page 583 For water MBTH Method* Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm the concentration Note: Samples must be and module number units and the zero 61.01...
Page 584 FORMALDEHYDE, continued Accurately Accurately Add the contents measure 25 mL of measure 25 mL of of one MBTH sample in a 50-mL formaldehyde-free Powder Pillow to the mixing cylinder (the water in a second blank. Stopper the prepared sample). 50-mL mixing cylinder.
Page 585 FORMALDEHYDE, continued 17 minutes 20 seconds Set a timer for a Immediately Wait 2 minutes 17-minute reaction shake the cylinder (15 minutes period and proceed vigorously for 20 remaining). Then immediately to Step 8. seconds. add the contents of one MBTH Powder Note: Important- begin Pillow to the prepared sample.
Page 586 FORMALDEHYDE, continued Just before 15 After the 15 Press: ZERO minutes has elapsed minutes has elapsed, (2 minutes place the blank in the The display will remaining), fill a cell holder. count down to 0. 10-mL cell to the Then the display will 10-mL line with the Note: In bright light, it show 0 µg/L and the...
Page 587 FORMALDEHYDE, continued ACCURACY CHECK Standard Additions Method a) Snap the neck off a Formaldehyde Voluette Ampule Standard Solution, 4000 mg/L. b) Use the TenSette Pipet to add 0.2 mL of standard to a 100-mL volumetric flask. Dilute to volume with formaldehyde-free water. Mix well.
Page 588 Other aldehydes give a positive interference. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagent. Testing 200 µg/L concentration solutions, the standard deviation was ±2.4 µg/L CH Testing zero concentration samples, the limit of detection was 2.9 µg/L...
Page 589 Water bath, 240 Vac, 50 Hz, 5 gal (19 L) . . . each ..24638-02 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 61-33...
Page 590 61-34...
Page 591 For boiler and cooling tower waters Ternary Complex Method Install module Press: After 2 seconds 61.01 the display will show in a DR/700. The display will show a program number, 610 nm concentration units, Note: If samples cannot and module number decimal position and 61.01...
Page 592 MOLYBDENUM, MOLYBDATE, LR continued Fill a 25-mL Add the contents Fill a 10-mL cell sample cell to the of one Molybdenum to the 10-mL line 20-mL line with the 1 Reagent Powder with prepared sample. Pillow. Cap Shake sample. the sample cell to Note: For proof of dissolve the reagents (the prepared...
Page 593 MOLYBDENUM, MOLYBDATE, LR continued Place the blank Press: Place the ZERO in the cell holder. developed sample in The display will the cell holder. Note: In bright sunlight count down to 0. Then the display will Note: In bright sunlight it may be necessary to show 0.00 mg/L and it may be necessary to...
Page 594 20 mL of the standard according to the above procedure. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.000 mg/L Mo concentration samples, the standard deviation was ±0.008 mg/L Mo Testing zero concentration samples, the limit of detection was 0.035 mg/L Mo...
Page 595 MOLYBDENUM, MOLYBDATE, LR continued Negative Interference: Level above which it interferes (mg/L) Iron Copper Chromium (Cr 4.5* Chloride 1,400 AMP (Phosphonate) Phosphonohydroxyacetic Acid Bisulfate 3,300 Nitrite 350* Aluminum Acrylates Alum Lignin Sulfonate Orthophosphate 4,500 Bicarbonate 5,650 EDTA 1,500 Borate 5,250 Ethylene Glycol 2% (by volume) Sulfite...
Page 596 ..1 ..each ... 968-00 DR/700 Filter Module Number 61.01... 1 ..each ..46261-00...
Page 597 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 61-41...
Page 598 61-42...
Page 599 Install module Press: After 2 seconds, number the display will show 61.01 The display will show a program number, in a DR/700. 610 nm the concentration and module number units and the zero Note: If samples cannot 61.01 prompt. If necessary,...
Page 600 0. Then the display will Note: Typical indoor lighting permits the show 0.00 mg/L and lighting permits the DR/700 to operate with the zero prompt will DR/700 to operate with the cell compartment turn off. the cell compartment cover open.
Page 601 NITROGEN, AMMONIA, continued Press: READ Table 1. Conversion Factors To convert reading from Multiply by The display will mg/L NH mg/L NH 1.29 count down to 0. mg/L NH mg/L NH 1.22 Then the display will show the results in mg/L NH as N.
Page 602 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents, Testing 0.400 mg/L NH as N concentration samples, the standard deviation was ±0.006 mg/L NH as N.
Page 603 Samples with severe interferences require distillation. Albuminoid nitrogen samples also require distillation. Hach recommends the distillation procedure using the Hach General Purpose Distillation Set. See Optional Apparatus listing. The distillation procedure is detailed in the Nitrogen, Ammonia - Nessler Method.
Page 604 Clippers, large ... . . 1 ..each ... 968-00 DR/700 Filter Module Number 61.01.
Page 605 Thermometer, -20 to 105 °C ... . . each ... 1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 61-49...
Page 606 61-50...
Page 607 The display will show or twice until the in a DR/700. 610 nm lower display shows and module number program number Note: The DR/700 must 61.01 61.000 be calibrated before After 2 seconds, the If necessary, press the sample analysis. See...
Page 608 NITROGEN, MONOCHLORAMINE, continued 15 minutes Promptly add the Wait 15 minutes. Place the cell contents of one While waiting, wipe labeled blank into the Monochloramine fingerprints, liquids, cell holder. Reagent Pillow to the etc. from the cells. cell labeled free Note: In bright sunlight ammonia and one to it may be necessary to...
Page 609 NITROGEN, MONOCHLORAMINE, continued Leave the Place the cell Press: READ monochloramine cell labeled free ammonia The display will in the cell holder. into the cell holder. count down to 0. Press twice. Then the display will ZERO Note: In bright light it show the free The display will show ammonia result in...
Page 610 61.01 and module number program number in a DR/700. 61.01 61.000 Note: The DR/700 must After 2 seconds, the If necessary, press the be calibrated before display will show a until the UP ARROW sample analysis. See...
Page 611 NITROGEN, MONOCHLORAMINE, continued Mix by vigorously Promptly fill a Invert the ampuls adding 40-50 mL of Monochloramine to dissolve the sample to the beaker AccuVac ampul from powder. labeled free each beaker. ammonia. Add 40-50 Note: For proof of mL of sample to the Note: Keep tips accuracy test a 0.50 beaker labeled...
Page 612 NITROGEN, MONOCHLORAMINE, continued Place the Place the ampul Press: READ AccuVac Adapter in filled from the beaker the cell holder. labeled The display will monochloramine into count down to 0. the cell holder. Then the display will show the Note: In bright light it monochloramine results in mg/L NH may be necessary to...
Page 613 100 mL with demineralized water. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.2 mg/L NH as N concentration samples, the standard deviation was ±0.010 mg/L NH Testing zero concentration samples, the limit of detection was 0.009 mg/L...
Page 614 NITROGEN, MONOCHLORAMINE, continued Manual. Standard 1 (S1) is made by pouring 10 mL of sample into a zeroing vial or 10-mL sample cell. Cap the cell. Edit the display to show 00.00 mg/L. For standard 2 (S2), edit the display to show 00.55 mg/L and 1.124 Abs.
Page 615 REQUIRED REAGENTS (Using AccuVac Ampuls) DR/700 Filter Module Number 61.01... 1 ..each ..46261-00 Free Ammonia AccuVac Reagent Set (12 tests) Includes: (1) 25230-25, (1) 26072-36 .
Page 616 Wipers, disposable, Kimwipes, 30x30 cm . . 280/box ..20970-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 61-60...
Page 617 Collect at Then the display will may be necessary to show 0.00 µg/L and least 40 mL of sample close the cell in a 50-mL beaker. the zero prompt will compartment cover. turn off. AccuVac is a Hach Company trademark. 61-61...
Page 618 OXYGEN, DISSOLVED, LR, continued Insert the Fill a Low Range Without inverting AccuVac Vial Dissolved Oxygen the ampul, Adapter into the cell AccuVac Ampul with immediately place the holder. sample. Refer to ampul cap filled with sampling and storage sample securely over for technique.
Page 619 OXYGEN, DISSOLVED, LR, continued Wipe the ampul Press: READ dry and immediately place the prepared The display will sample in the cell count down to 0. holder. Then the display will show the results in µg/L dissolved Note: In bright light it oxygen.
Page 620 STATISTICAL EVALUATION A single operator repetitively tested samples of the same solutions, using one DR/700 and two representative lots of AccuVacs. Testing 300 µg/L O samples, the standard deviation was ±27.7 µg/L O Testing zero concentration samples, the limit of detection was 16.1 µg/L O...
Page 621 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac, Vial, DR/700 ..each ..46025-00 Cap for 10- and 25-mL sample cells .
Page 622 61-66...
Page 623 Method 8000 OXYGEN DEMAND, CHEMICAL (COD) (0 to 1,500 and 0 to 15,000 mg/L) For water, wastewater and seawater Reactor Digestion Method*; USEPA approved for reporting (0 to 1,500 mg/L range)† DIGESTION 2 minutes Remove cap of a Homogenize a Turn on the COD COD Digestion 100 mL sample for 2...
Page 624 OXYGEN DEMAND, CHEMICAL, continued cap tightly. Rinse the Hold the vial at a Hold the vial by COD vial with 45-degree angle. the cap and over a demineralized water Pipet 2.00 mL sink. Invert several and wipe the vial (0.2 mL for 0 to times to mix the clean with a paper 15,000 range) of...
Page 625 OXYGEN DEMAND, CHEMICAL, continued 2 hours Prepare a blank Heat the vials for Turn the reactor by repeating Steps 3 2 hours. off. Wait 20 minutes to 6, substituting 2.00 for the vials to cool to mL (0.2 mL for 0 to Note: Many wastewater C or less.
Page 626 OXYGEN DEMAND, CHEMICAL, continued Invert each vial several times while still warm. Place the vials into a rack. Wait until the vials have cooled to room temperature. Note: If a pure green color appears in the reacted sample, the reagent capacity may have been exceeded.
Page 627 COLORIMETRIC DETERMINATION, 0 to 1,500 and 0 to 15,000 mg/L COD Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm the concentration and module number units and the zero 61.01...
Page 628 Place the sample ZERO of the sample vial vial in the adapter The display will with a towel. with the Hach logo count down to 0. facing the front of the Then the display will instrument. Note: Wiping with a show 0.00 mg/L, and...
Page 629 10,000 mg/L COD. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of reagents. For 0-1,500 range, a 1200 mg/L COD sample was tested and the standard deviation was ±29 mg/L COD.
Page 630 OXYGEN DEMAND, CHEMICAL, continued INTERFERENCES Chloride is the primary interference when determining COD concentration. Each COD vial contains mercuric sulfate that will eliminate chloride interference up to the level specified in column 1 in the table below. Dilute samples with higher chloride concentrations. Dilute the sample enough to reduce the chloride concentration to the level given in column 2.
Page 631 COD Reactor, 120/240 Vac ..1 ..each ..45600-00 COD Vial Adapter, DR/700 . . . 1 ..each ..46008-00 DR/700 Filter Module Number 61.01.
Page 632 COD Disposal Information Brochure ......4144 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. 61-76...
Page 633 For water Indigo Method (Using AccuVac Ampuls) Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm the concentration Note: Samples must be and module number units and the zero 61.01...
Page 634 OZONE, continued Collect at least Fill one Indigo Quickly invert 40 mL of ozone-free Ozone Reagent both ampuls several water (blank) in AccuVac Ampul with times to mix. Wipe another 50-mL the sample and one off any liquid or beaker. ampul with the blank.
Page 635 OZONE, continued Place the Press: READ AccuVac ampul containing the blank The display will into the cell holder. count down to 0. Then the display will Note: In bright sunlight, show the result in mg/ L ozone (O it may be necessary to close the cell compartment cover.
Page 636 Low Range A single operator repetitively tested samples of the same solutions, using one DR/700 and two representative lots of AccuVacs. Testing 0.15 mg/ samples, the standard deviation was ±0.009 mg/L O Testing zero concentration samples, the limit of detection was 0.024 mg/L.
Page 637 AccuVac Snapper Kit ....each ..24052-00 Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Cap for 10- and 25-mL sample cells .
Page 638 61-82...
Page 639 For water, wastewater and seawater Methylene Blue Method*; USEPA accepted for reporting** Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm concentration units, Note: Samples must be and module number decimal position and 61.01...
Page 640 Note: Typical indoor Immediately swirl to mix. lighting permits the DR/700 to operate with Note: A pink color will the cell compartment develop. Then the cover open. In bright solution will turn blue if sunlight, it may be sulfide is present.
Page 641 Examination of Water and Wastewater, 17th ed., page 4-195. STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions using one DR/700, matched sample cells and two representative lots of testing reagents, Testing 0.595 mg/L S concentration samples, the standard deviation was ±0.008 mg/L S Testing zero concentration samples, the limit of detection was 0.010 mg/L S...
Page 642 SULFIDE, continued INTERFERENCES For turbid samples, prepare a sulfide-free blank as follows. Use it in place of the demineralized water blank in the procedure. a) Measure 25 mL of sample into a 50-mL erlenmeyer flask. b) Add Bromine Water dropwise with constant swirling until a yellow color remains.
Page 643 Water and Wastewater, 18th ed..each ..22708-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 61-87...
Page 644 61-88...
Page 645 Method 8028 SURFACTANTS, ANIONIC (0 to 0.300 mg/L) For water, wastewater and seawater (Also called Detergents) Crystal Violet Method* 5 seconds Measure 300 mL Add 10 mL of Add the contents of sample in a 500-mL Sulfate Buffer of one Detergent separatory funnel.
Page 646 Install module Press: After 2 seconds, number the display will show 61.01 The display will show a program number, in a DR/700. 610 nm the concentration and module number units, decimal 61.01 position, and zero prompt. If necessary, press the...
Page 647 0. Then the display will Note: Typical indoor lighting permits the show 0.000 mg/L and lighting permits the DR/700 to operate with the zero prompt will DR/700 to operate with the cell compartment turn off. the cell compartment cover open.
Page 648 STATISTICAL EVALUATION A single operator repetitively tested samples of two prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.180 mg/L as LAS concentration samples, the standard deviation was ±0.0036 mg/L as LAS.
Page 649 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 61-93...
Page 650 61-94...
Page 651 Zincon Method*; USEPA approved for reporting (digestion is required; see Section 1)†. Install module Press: After 2 seconds, 61.01 the display will show in a DR/700. The display will show a program number, 610 nm concentration units Note: If sample cannot and module number and the zero prompt.
Page 652 ZINC, continued Fill a 25-mL Add the contents Measure 10 mL of graduated mixing of one ZincoVer 5 the solution into a cylinder to the 20-mL Reagent Powder 10-mL sample cell mark with sample. Pillow. Cap. Invert (the blank). Cap the several times until the cell.
Page 653 ZINC, continued 30 seconds Add 0.5 mL of Stopper the Pour the prepared cyclohexanone to the cylinder (the prepared sample into a 10-mL remaining solution in sample). Shake for sample cell. Cap the the cylinder. 30 seconds. cell. Note: Use a plastic Note: The sample color dropper because rubber will be reddish-brown,...
Page 654 ZINC, continued Within 10 Press: READ minutes of the three- minute period, place The display will the prepared sample count down to 0. in the cell holder. Then the display will show the results in Note: In bright light it mg/L zinc (Zn).
Page 655 Prepare this solution daily. STATISTICAL EVALUATION A single operator repetitively tested samples of two prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.80 mg/L Zn concentration samples, the standard deviation was ±0.0068 mg/L Zn.
Page 656 25 mL ..1 ..each ... 1896-40 DR/700 Filter Module Number 61.01... 1 ..each ..46261-00 Dropper, plastic, 0.5 and...
Page 657 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. *Contact Hach for larger sizes. †100 Tests equals 100 samples and 100 blanks.
Page 658 61-102...
Page 659 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 660 Table of Contents for 690-nm parameters Oxygen, Dissolved, SHRDO, AccuVac Ampul ....69-1 Tannin and Lignin ........69-7...
Page 661 Install module Press: After 2 seconds, number the display will show 69.01 The display will show a program number, in a DR/700. 690 nm the concentration and module number units and the zero Note: The sample must 69.01 prompt. If necessary,...
Page 662 OXYGEN, DISSOLVED, SHR, continued 30 seconds Fill a blue ampul Fill a High Range Without inverting cap with sample. Dissolved Oxygen the ampul, Then fill a cell with AccuVac Ampul with immediately place the 10 mL of sample (the sample. ampul cap that has blank).
Page 663 OXYGEN, DISSOLVED, SHR, continued Press: ZERO Insert the Place the AccuVac Vial prepared sample in The display will Adapter into the cell the cell holder. count down to 0. holder. Then the display will Note: In bright sunlight show 0.00 mg/L and it may be necessary to the zero prompt will close the cell...
Page 664 STATISTICAL EVALUATION A single operator repetitively tested samples of the same solutions, using one DR/700 and two representative lots of AccuVac Ampuls. Testing samples, the standard deviation was ±1.08 mg/L O 24.0 mg/L O Testing zero concentration samples, the limit of detection was 1.60 mg/L...
Page 665 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 69-5...
Page 666 69-6...
Page 667 For water, wastewater, boiler water Tyrosine Method* Install module Press: After 2 seconds, 69.01 the display will show in a DR/700. The display will show a program number, 690 nm the concentration Note: If samples cannot and module number units and the zero 69.01...
Page 668 TANNIN AND LIGNIN, continued Fill a 25-mL cell Fill another 25-mL Pipet 0.5 mL of to the 25-mL line cell to the 25-mL line TanniVer 3 Tannin- with demineralized with sample (the Lignin Reagent into water (the blank). prepared sample). each cell.
Page 669 TANNIN AND LIGNIN, continued 25 minutes Pipet 5.0 mL of Wait 25 minutes. Place the blank in Sodium Carbonate the cell holder. Solution into each Note: Use a 10-mL cell cell. Cap and invert several times to mix. for the blank and the sample in bright Note: A blue color will sunlight.
Page 670 TANNIN AND LIGNIN, continued Press: Place the Press: ZERO READ prepared sample in the The display will cell holder. The display will count down to 0. count down to 0. Then the display will Note: Use a 10-mL cell Then the display will show 0.00 mg/L and show the results in for the blank and the...
Page 671 STATISTICAL EVALUATION A single operator repetitively tested samples of two prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 6.0 mg/L tannic acid concentration samples, the standard deviation was ±0.042 mg/L tannic acid.
Page 672 Spoon, measuring, 0.2 g ....each ... . 638-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 69-12...
Page 673 Warranties do not apply to limited-life electrical components such as batteries. Full warranty information is located on the reverse side of Hach invoices. IMPORTANT NOTICE A lamp intensity adjustment must be performed: •Before first or initial use...
Page 674 Table of Contents for 810-nm parameters Copper, Autocatalytic (user calibration) ..... . 81-1 Phosphonates ......... . . 81-5 Phosphorus, Acid Hydrolyzable, Test ‘N Tube™...
Page 675 Method 8117 COPPER, AUTOCATALYTIC (0 to 3.00 g/L) For finishing baths Colorimetric Method* Pipet 5.0 mL of Pipet 3.0 mL of Add the contents demineralized water bath sample and of one Acid Reagent into a 16-mm screw- 2.0 mL of Powder Pillow to top culture tube (the demineralized water...
Page 676 Wait until any Full insert a COD Install module foaming Vial Adapter into the 81.01 (effervescence) stops. cell holder with the in a DR/700. Cap and shake to tabs in the square dissolve all the slot. powder. Press: After 2 seconds,...
Page 677 The display will the adapter with the The display will count down from 0. Hach logo facing the count down to 0. Then the display will front of the Then the display will show 0.00 g/L and the instrument.
Page 678 Pipet Tips, for 19700-10 TenSette Pipet ..50/pkg ..21997-96 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 81-4...
Page 679 Install module Press: After 2 seconds, number the display will show 81.01 The display will show a program number, in a DR/700. 810 nm the concentration and module number units and the zero 81.01 prompt. If necessary, Note: If samples cannot...
Page 680 PHOSPHONATES, continued Choose a sample Split the diluted Add the contents size from Table 1 sample by pouring 25 of one Potassium below. Pipet the mL into each of two Persulfate For chosen sample sample cells. Phosphonate Powder volume into a 50-mL Pillow to one of the graduate mixing sample cells (the...
Page 681 PHOSPHONATES, continued 10 minutes Insert the Turn the lamp on. Turn the UV lamp ultraviolet (UV) lamp Wait for 10 minutes. off and remove from into the prepared the sample cell. Note: Phosphonates are sample. The other sample is the blank. converted to orthophosphate in this Note: UV safety goggles...
Page 682 PHOSPHONATES, continued 2 minutes Add the Wait 2 minutes. Place the blank contents of one in the cell holder. Note: If the sample is PhosVer 3 Phosphate Note: Use a 10-mL cell Reagent Powder colder than 15 C, 4 Pillow to each sample for the blank and minutes are required for cell.
Page 683 PHOSPHONATES, continued Press: Place the Press: ZERO READ prepared sample in The display will the cell holder. The display will count down to 0. count down to 0. Then the display will Then the display will Note: Use a 10-mL cell show 0.0 mg/L and show the results in for the blank and...
Page 684 PHOSPHONATES, continued Multiply the result by the appropriate value in Table 2 to obtain the actual concentration of the sample. Note: Results may be expressed in terms of active phosphonic acid by picking the appropriate conversion factor and using the equation in Table 3.
Page 685 PHOSPHONATES, continued SAMPLING AND STORAGE Collect sample in clean plastic or glass bottles that have been cleaned with 1:1 Hydrochloric Acid Solution and rinsed with demineralized water. Do not use a commercial detergent. If prompt analysis is impossible, adjust the pH to 2 or less with about 2 mL of sulfuric acid, ACS, per liter of sample.
Page 686 Goggles, UV safety ..1 ..each ..21134-00 DR/700 Filter Module Number 61.01... 1 ..each ..46261-00 Pipet, volumetric, Class A, 5.00 mL .
Page 687 UV Lamp......each ..20823-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 81-13...
Page 688 81-14...
Page 689 ™ Turn on the COD Install module Press: Reactor. Heat to 81.01 103 to 106 C. Place in a DR/700 The display will show the plastic shield in 810 nm front of the reactor. Note: Refer to and module 81.01...
Page 690 PHOSPHORUS, ACID HYDROLYZABLE, continued After 2 seconds, Fill a Test ’N Add 2 mL of the display will show Tube Cuvette vial 1.00 N H to the a program number, with 5 mL of sample. sample vial. the concentration units and the zero Note: For proof of Note: A 2-mL Repipet prompt.
Page 691 Place the sample Press: ZERO outside of the vial vial in the adapter with a towel. with the Hach logo The display will facing the front of the count down to 0. Note: Wiping with a instrument. Then the display will show 0.000 mg/L,...
Page 692 The display will Place the prepared count down to 0. sample into the Then the display will adapter with the Hach show the results in logo facing the front mg/L PO of the instrument. Note: Wiping with a...
Page 693 PHOSPHORUS, ACID HYDROLYZABLE, continued CALIBRATION PROCEDURE 1) If the DR/700 is on and in a pre-programmed method, proceed to Step 3. If it is off, press . The upper display will show the wavelength and the lower display will show the module installed and the software version number.
Page 694 PHOSPHORUS, ACID HYDROLYZABLE, continued 12) Press the key. The left digit and the S2 annunciator RIGHT ARROW will flash. Use the arrow keys to enter the correct absorbance difference. If a negative number appears, continue to press the UP ARROW until a positive 0.570 appears.
Page 695 PRECISION DR/700: In a single laboratory, using a standard solution of 4.20 mg/L PO and two lots of reagent with a DR/700, a single operator obtained a standard deviation of ±0.11 mg/L PO SAMPLE DISPOSAL INFORMATION The phosphate vials contain only materials that are non-regulated for disposal.
Page 696 COD Reactor, 120/240 Vac ..1 ..each ...45600-00 COD Vial Adapter, DR/700..1 ..each ...46008-00 Funnel, micro .
Page 697 Pipet Tips, for 19700-10 TenSette Pipet ..50/pkg ..21997-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 81-23...
Page 699 PhosVer 3 Method; Test ’N Tube Procedure ™ Install module Press: After 2 seconds, 81.01 the display will show in a DR/700 The display will show a program number, 810 nm the concentration and module units and the zero Note: Refer to 81.01...
Page 700 Place the sample COD Vial Adapter of the sample vial vial in the adapter into the cell holder with a towel. with the Hach logo with the tabs in the facing the front of the square slot. Note: Wiping with a instrument.
Page 701 PHOSPHORUS, REACTIVE, continued Press: Remove the cap Using a funnel, ZERO from the vial. add the contents of The display will one PhosVer 3 count down to 0. Phosphate Reagent Then the display will Powder Pillow for show 0.0 mg/L, and 10-mL samples to the zero prompt will the vial.
Page 702 PO CALIBRATION PROCEDURE 1) If the DR/700 is on and in a pre-programmed method, proceed to Step 3. If it is off, press the key. The upper display will show the wavelength and the lower display will show the module installed and the software version number.
Page 703 PHOSPHORUS, REACTIVE, continued 9) Press . The display will count down and then show another ZERO four-digit concentration value. The S2 annunciator will begin flashing. 10) Press the RIGHT ARROW key. The left digit will begin flashing. Press the arrow keys to edit the display to read 5.000 for PO 11) Press .
Page 704 PHOSPHORUS, REACTIVE, continued ACCURACY CHECK Standard Additions Method a) Snap the neck of a Phosphate Voluette Ampule Standard, 25 mg/L as P. b) Use the TenSette Pipet to add 0.1 mL, 0.2 mL and 0.3 mL, respectively, to three 5-mL aliquots of a water samples. Mix well. c) Analyze each sample as described in the procedure.
Page 705 COD Reactor, 120/240 Vac . . . 1 ..each ... 45600-00 COD Vial Adapter, DR/700 . . . 1 ..each ..46008-00 DR/700 Filter Module 81.00.
Page 706 Pipet Tips, for 19700-10 TenSette Pipet ..50/pkg ..21997-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 81-32...
Page 707 ™ Turn on the COD Install module Press: Reactor. Heat to 81.01 103 to 106 C. Place in a DR/700. The display will show the plastic shield in 810 nm front of the reactor. Note: Refer to and module 81.01...
Page 708 PHOSPHORUS, TOTAL, continued After 2 seconds, Fill a Test ’N Add 2 mL of the display will show Tube Cuvette vial 1.00 N H to the a program number, with 5 mL of sample. sample vial. the concentration units and the zero Note: For proof of Note: A 2-mL Repipet prompt.
Page 709 The display will with the tabs in the the sample vial in the count down to 0. square slot. adapter with the Hach Then the display will logo facing the front show 0.0 mg/L, and of the instrument. the zero prompt will turn off.
Page 710 The display will Place the prepared count down to 0. sample into the Then the display will adapter with the Hach show the results in logo facing the front mg/L PO of the instrument. Note: Wiping with a...
Page 711 PHOSPHORUS, TOTAL, continued CALIBRATION PROCEDURE 1) If the DR/700 is on and in a pre-programmed method, proceed to Step 3. If it is off, press the key. The upper display will show the wavelength and the lower display will show the module installed and the software version number.
Page 712 PHOSPHORUS, TOTAL, continued 12) Press the key. The left digit and the S2 RIGHT ARROW annunciator will flash. Use the arrow keys to enter the correct absorbance difference. Press the key until a positive UP ARROW 0.570 appears. 13) Press .
Page 713 PRECISION DR/700: In a single laboratory, using a standard solution of 4.20 mg/L PO and two lots of reagent with a DR/700, a single operator obtained a standard deviation of ±0.11 mg/L PO SAMPLE DISPOSAL INFORMATION The phosphate vials contain only materials that are non-regulated for disposal.
Page 714 COD Reactor, 120/240 Vac . . . 1 ..each ..45600-00 COD Vial Adapter, DR/700 . . 1 ..each ..46008-00 DR/700 Filter Module Number 81.01.
Page 715 Pipet Tips, for 19700-10 TenSette Pipet ..50/pkg ..21997-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 81-41...
Page 716 81-42...
Page 717 Method 8180 PHOSPHORUS, ACID HYDROLYZABLE For water, wastewater, seawater Hydrolysis to Orthophosphate Method* 30 minutes Measure 25 mL of Add 2.0 mL of Place the flask sample into a 50-mL Sulfuric Acid (the prepared sample) erlenmeyer flask Solution, 5.25 N. on a hot plate.
Page 718 PHOSPHORUS, ACID HYDROLYZABLE, continued Cool the prepared Add 2.0 mL of Pour the prepared sample to room 5.0 N Sodium sample into a temperature. Hydroxide Solution. graduated cylinder. Swirl to mix. Add demineralized water rinsings from Note: Use the 1-mL the flask to return the volume to 25 mL.
Page 719 PHOSPHORUS, ACID HYDROLYZABLE continued SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed immediately. If prompt analysis is not possible, samples may be preserved up to 24 hours by cooling to 4 °C (39 °F). Warm to room temperature before testing.
Page 720 Meter, EC10, portable ....each ..50050-00 For Technical Information Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. *Contact Hach for larger sizes. 81-46...
Page 721 (0 to 2.50 mg/L PO For water, wastewater, seawater (also called: Orthophosphate) PhosVer 3 (Ascorbic Acid) Method* (Powder Pillows or AccuVac Ampuls), USEPA accepted for reporting** USING POWDER PILLOWS Install DR/700 Press: After 2 seconds, module the display will show 81.01...
Page 722 PHOSPHORUS, REACTIVE, continued 2 minutes Fill a 10-mL cell Add the contents Wait two minutes. to the 10-mL line of one PhosVer 3 Note: An 8-10 minute with sample. Phosphate Powder Pillow to the sample reaction period should cell (the prepared Note: For proof of be used if determining sample).
Page 723 PHOSPHORUS, REACTIVE, continued Fill a 10-mL cell Place the blank in Press: ZERO to the 10-mL line the cell holder. with sample (the The display will Note: In bright sunlight blank). count down to 0 Then the display will it may be necessary to show 0.00 mg/L, and close the cell the zero prompt will...
Page 724 PHOSPHORUS, REACTIVE, continued USING ACCUVAC AMPULS Install DR/700 Press: After 2 seconds, module the display will show 81.01 The display will show a program number, in the instrument. 810 nm concentration units, and module number decimal position and 81.01 the zero prompt. If...
Page 725 PHOSPHORUS, REACTIVE, continued 30 seconds Fill a cell with Fill a PhosVer 3 Place an ampul 10 mL of sample (the Phosphate AccuVac cap securely over the blank). Cap. Collect Ampul with sample. tip of the ampul. at least 40 mL of Shake the ampul for sample in a 50-mL Note: Keep the tip...
Page 726 PHOSPHORUS, REACTIVE, continued Insert the Place the Press: READ AccuVac Vial prepared sample in Adapter into the cell the cell holder. The display will holder. count down to 0. Note: In bright sunlight Then the display will show the results in it may be necessary to mg/L phosphate close the cell...
Page 727 PHOSPHORUS, REACTIVE, continued ACCURACY CHECK Standard Additions Method a) Snap the neck off a Phosphate Voluette Ampule Standard Solution, 50 mg/L PO b) Use the TenSette Pipet to add 0.1 mL, 0.2 mL and 0.3 mL of standard, respectively, to three 25-mL water sample. Mix each thoroughly. (For AccuVac Ampuls use 50-mL Beakers.) c) Analyze each sample as described above.
Page 728 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 1.60 mg/L PO concentration samples, the standard deviation was ±0.007 mg/L PO Testing zero concentration samples, the limit of detection was 0.019 mg/L PO...
Page 729 OPTIONAL APPARATUS Adapter, AccuVac Vial, DR/700 ..each ..46025-00 Ampule Breaker Kit ....each ..21968-00 Cap for 10- and 25-mL sample cells .
Page 730 81-56...
Page 731 Method 8190 PHOSPHORUS, TOTAL For water, wastewater and seawater (also called Organic and Acid Hydrolyzable) Acid Persulfate Digestion Method*; USEPA accepted for reporting Measure 25 mL Add the contents Add 2.0 mL of of sample into a of one Potassium 5.25 N Sulfuric Acid 50-mL erlenmeyer Persulfate Powder...
Page 732 PHOSPHORUS, TOTAL, continued 30 minutes Place the flask on Cool the sample Add 2.0 mL of a hot plate. Boil to room temperature. 5.0 N Sodium gently for 30 minutes. Hydroxide Solution. Swirl to mix. Note: Maintain the Note: Use the 1-mL volume near 20 mL by calibrated dropper adding small amounts of...
Page 733 PHOSPHORUS, TOTAL, continued Pour the sample into a 25-mL graduated cylinder. Using demineralized water rinsings from the flask, return the volume in the cylinder to 25 mL. Proceed with a reactive phosphorus test of the expected total phosphorus concentration range. Note: Results of the reactive phosphorus test at this point will include...
Page 734 PHOSPHORUS, TOTAL, continued SAMPLING AND STORAGE Most reliable results are obtained when samples are analyzed immediately. If prompt analysis is not possible, samples may be preserved up to 24 hours by cooling to 4 °C (39 °F). Warm to room temperature before testing.
Page 735 Meter, EC10, portable ....each ..50050-00 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A - Contact the Hach office or distributor serving you. 81-61...
Page 736 81-62...
Page 737 For water and seawater Heteropoly Blue Method* Install module Press: After 2 seconds, 81.01 the display will show in a DR/700. The display will show a program number, 810 nm concentration units, Note: If samples cannot and module number decimal position and 81.01...
Page 738 SILICA, LR, continued 4 minutes Fill a 10-mL cell Add 10 drops of Wait 4 minutes to the 10-mL line Molybdate 3 Reagent and remove cap. with sample. to the cell. Cap and Note: Reaction time invert several times to mix.
Page 739 SILICA, LR, continued 1 minute Press: Add the Wait 1 minute. ZERO contents of one The display will Amino Acid F count down to 0. Reagent Powder Then the display will Pillow to the cell. show 0.000 mg/L and This is the prepared the zero prompt will sample.
Page 740 STATISTICAL EVALUATION A single operator repetitively tested samples of two laboratory prepared solutions, using one DR/700, matched sample cells and two representative lots of testing reagents. Testing 0.750 mg/L SiO concentration samples the standard deviation was ±0.0161 mg/L SiO Testing zero concentration samples, the limit of detection was 0.0144 mg/L SiO...
Page 741 ..1 ..each ... . 968-00 DR/700 Filter Module Number 81.01 ... . . 1 ..each ..46281-00...
Page 742 C... . . each ... 1877-01 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or the distributor serving you. 81-68...
Page 743 All other apparatus is available from Hach. Install module Press: After 2 seconds, 81.01 the display will show in a DR/700. The display will show a program number, 810 nm the concentration and module number units and the zero 81.01 prompt.
Page 744 SUSPENDED SOLIDS, continued Fill a 25-mL cell Place the blank in Press: ZERO to the 25-mL line the cell holder. with tap or The display will Note: Use 10-mL cells demineralized water count down to 0. (the blank). Then the display will for the blank and the show 0.00 mg/L and prepared sample in...
Page 745 SUSPENDED SOLIDS, continued Slowly and Place the Press: READ gently invert and prepared sample into swirl the prepared the cell holder. The display will sample to uniformly count down to 0. suspend any residue. Note: Use 10-mL cells Then the display will Avoid vigorous show the results in for the blank and the...
Page 746 Sample Cell, 25-mL with screw cap ..6/pkg ..24019-06 For Technical Assistance, Prices and Ordering In the U.S.A. - Call 800-227-4224 toll-free for more information. Outside the U.S.A. - Contact the Hach office or distributor serving you. 81-72...
Page 747 SECTION III Technical Support...
Page 749: Technical Training Workshops
To obtain the latest workshop listing and schedule, call toll-free (800) 227-4224 and ask for the Training Center Administrator or contact the Hach office or distributor serving you. TECHNICAL AND APPLICATION ASSISTANCE A staff of trained technical service representatives and chemists are available to handle your specific questions and make application recommendations.
Page 750: Ordering Information
Published three times per year, News and Notes for the Analyst contains updates on federal regulations, in-depth discussions of chemical methods, application notes for instrument or test procedures and a Hach product update. Products for Analysis catalog describes laboratory, portable, and on-line...
Page 751 Provides answers to the questions: What is BOD? What is the significance of BOD? How is BOD measured? How reliable is the BOD test? Chemical Oxygen Demand Hach COD system for Wastewater Testing. Describes a semi-micro COD monitoring system with ready-to-use sample vials. Includes a cost comparison.
Page 752 Digestion Acid Digestion Using the Hach Digesdahl Digestion Apparatus. Sample Preparation for Protein and Elemental Analysis. By Scott V. Brayton, 1988. Focuses on digestion using sulfuric acid and hydrogen peroxide, a modification of the Kjeldahl method, which is suitable for determining a range of metals and nonmetals including nitrogen.
Page 753 General Chemistry “Measurement of Color and Insoluble Matter in Reagent Chemicals”. By Clifford C. Hach. Reprinted from American Laboratory, June, 1983. When results from colorimetric and turbidimetric tests are compared with traditional gravimetric measurements, the colorimetric tests are shown to offer more sensitive and useful information about insoluble and color-related impurities.
Page 754 “pH Measurement Problems Solved”. By Gale McGaha Miller. Reprinted from American Laboratory News Edition, October, 1988. This article compares the conventional reference junction used in most pH electrodes and the free diffusion reference junction used in Hach pH electrodes. Plating and Surface Finishing “Heavy Metal Removal Using Dithiocarbamates”.
Page 755 This article describes the use of an on-line instrument for measuring chlorine in effluent at a 12 mgd facility. “Nonradioactive Silica Can Be Monitored On-line”. By Clifford Hach. Reprinted from Power, Vol. 128, No. 1, 1984. Combining turbidity measurement with on-line measurement of reactive silica provides a much needed tool for protection of high-pressure- system components.
Page 756 Turbidity Measurement “Turbidimetric Measurements of High Purity Water”. By Clifford C. Hach and Lawrence M. Liggett. Reprinted from Ultrapure Water, Vol. 2 No. 4, July/August, 1985. Describes a very sensitive instrument technique for detecting particles in liquids, including particles too small to be detected by the most sensitive particle counters.
Page 757 National Pollution Discharge Elimination System testing requirements and still stay within budget. “Weigh the Advantages of Hach’s Prepared Reagents”. Compares the cost of using reagents prepared by a manufacturer to the cost of using do-it-yourself reagents for chlorine analysis.
Page 758 3-10...
Page 759: How To Order
Outside the United States Hach maintains a network of dealers and distributors throughout the world. Obtain assistance from a nearby Hach distributor or: Hach Company World Headquarters P.O.
Page 760 Shipment Hach products are shipped FOB from the shipping point. If you do not specify a method of shipment, our staff will determine the best means of transportation consistent with Department of Transportation and postal service regulations.
Page 761: Service
Customers may send remittance with orders or we can ship C.O.D. if preferred. Warranty Hach warrants its products to be of high quality, to be free of material defects on the date of shipment and to be as specified. Full warranty information is on the back of Hach invoices.
Page 762 ONLY. They may not be used as drugs, cosmetics or food additives. MSDS Hach Material Safety Data Sheets, among the most complete and informative in the industry, provide comprehensive safety data essential for day-to-day operations and safety training.