Hach DR/700 Procedures Manual page 203

PHENOLS
For water, wastewater and seawater
4-Aminoantipyrine Method*, USEPA accepted for reporting
(distillation is required; see Section 1)**
1.
Measure 300 mL
of demineralized
water in a 500-mL
graduated cylinder.
Note: Analyze samples
within four hours to
avoid oxidation; see
Sampling and Storage
following these steps.
4.
Pour the
measured sample into
another 500-mL
separatory funnel (the
prepared sample).
*Adapted from Standard Methods for the Examination of Water and Wastewater
**Procedure is equivalent to USEPA Method 420.1 for wastewater
(0 to 0.200 mg/L)
2.
Pour the
measured
demineralized water
into a 500-mL
separatory funnel (the
blank).
5.
Add 5 mL of
Hardness 1 Buffer to
each separatory
funnel. Stopper.
Shake to mix.
45-31
Method 8047
3.
Measure 300 mL
of sample in a 500-mL
graduated cylinder.
Note: For proof of
accuracy, a phenol
standard solution
(preparation given in
Accuracy Check) can be
used in place of the
sample.
6.
Add the contents
of one Phenol Reagent
Powder Pillow to each
separatory funnel.
Stopper. Shake to
dissolve.
Note: Spilled reagent
will affect test accuracy
and is hazardous to skin
and other materials.