Applied Biosystems StepOne Installation, Networking, And Maintenance page 67

5a
5b
5c
Notes
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Applied Biosystems StepOne
Installation, Networking, and Maintenance Guide
5. Review the standard curve:
Select the Standard Curve tab.
a.
Click the upper-right corner of the Plate Layout to select all wells.
b.
Confirm that the R2 value is greater than or equal to 0.990.
c.
If the R2 value is less than 0.990, repeat the RNase P experiment using a different
RNase P plate. If the problem persists, contact Applied Biosystems as explained in
"How to Obtain Support" on page
6. Click Finish, click Yes when prompted to save the experiment.
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and StepOnePlus Real-Time PCR Systems
Chapter 3 Install the Colocated Layout
Perform the RNase P Experiment
x.
45