Preface
Purpose of This Guide
Guide
Applied Biosystems StepOne
System Software Help
Text Conventions
User Attention
Words
Safety Alert Words
viii
™
Real-Time PCR
This guide uses the following conventions:
• Bold text indicates user action. For example:
Type 0, then press Enter for each of the remaining fields.
• Italic text indicates new or important words and is also used for emphasis. For example:
Before analyzing, always prepare fresh matrix.
• A right arrow symbol ( ) separates successive commands you select from a drop-down
or shortcut menu. For example:
Select File Open.
Two user attention words appear in Applied Biosystems user documentation. Each word
implies a particular level of observation or action as described below:
– Provides information that may be of interest or help but is not critical to the use of
Note:
the product.
– Provides information that is necessary for proper instrument operation,
IMPORTANT!
accurate chemistry kit use, or safe use of a chemical.
Examples of the user attention words appear below:
The Calibrate function is also available in the Control Console.
Note:
To verify your client connection, you need a valid user ID.
IMPORTANT!
Safety alert words also appear in user documentation. For more information, see
Alert Words"
on
page
xi.
Applied Biosystems StepOne
Purpose and Audience
Explains how to use the StepOne
• Set up, run, and analyze experiments using the
™
StepOne
and StepOnePlus
• Monitor networked StepOne
instruments.
™
• Calibrate StepOne
and StepOnePlus
instruments.
• Verify the performance of StepOne
™
StepOnePlus
instruments with an RNase P run.
Intended for:
• Laboratory staff and principal investigators who
perform experiments using the StepOne
™
StepOnePlus
system.
• Laboratory staff responsible for the installation and
maintenance of the StepOne
system.
™
and StepOnePlus
Installation, Networking, and Maintenance Guide
™
software to:
™
systems.
™
™
and StepOnePlus
™
™
and
™
or
™
™
or StepOnePlus
"Safety
™
Real-Time PCR Systems
PN
NA
Need help?
Do you have a question about the StepOne and is the answer not in the manual?