Dye Calibration Failure - Applied Biosystems StepOne Installation, Networking, And Maintenance

Appendix A How to Troubleshoot the Installation

Dye Calibration Failure

Dye Calibration Failure

Troubleshooting
Workflow
Notes
134
Use this topic to troubleshoot failed dye calibrations.
Start
Does the instrument
complete the dye
calibration?
Yes
Does the
calibration pass?
Yes
Do the dye spectra
peak in the correct
filters?
Applied Biosystems StepOne
Reattempt the calibration. If the
problem persists, contact
Applied Biosystems as explained in
No
"How to Obtain Support" on page
One or more wells
produced spectra:
• Beyond the detectable
No
range for the system, or
• In more than one filters
If the peaks of one or more spectra appear in the wrong
filter, confirm that:
• The spectral calibration plate was run in the correct
orientation (the plate was not rotated 180° when run).
No
• The correct dyes were loaded onto the spectral
calibration plate, if you are running a custom plate.
™
and StepOnePlus
Installation, Networking, and Maintenance Guide
x.
Review the spectra for
irregularities as explained in
"Analyze the Calibration Data"
on page 106.
™
Real-Time PCR Systems