Applied Biosystems StepOne Installation, Networking, And Maintenance page 162

Glossary
cycling stage
data collection
∆
delta Rn ( Rn)
derivative reporter
(− Rn' )
Design Wizard
diluent
Diluted Sample
Concentration
(10✕ for Reaction
Mix)
dilution factor
dissociation curve
EFF%
endogenous
control
endpoint read
140
In the thermal profile, a stage that is repeated. A cycling stage is also called an
amplification stage. For cycling stages, you can enable AutoDelta settings. See also
amplification stage.
A process during the instrument run in which an instrument component detects
fluorescence data from each well of the reaction plate. The instrument transforms the
signal to electronic data, and the data are saved in the experiment file. In the StepOne
software, a data collection point is indicated by an icon in the thermal profile:
• Data collection on:
• Data collection off:
See baseline-corrected normalized reporter
The negative first-derivative of the normalized fluorescence generated by the reporter
during PCR amplification. In the derivative reporter (–Rn' ) vs. temperature melt curve,
the derivative reporter signal is displayed in the y-axis.
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A feature in the StepOne
through best practices as you enter your experiment design.
A reagent used to dilute a sample or standard before adding it to the PCR reaction. The
diluent can be water or buffer.
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In the StepOne software, a field displayed on the Sample Dilution Calculations tab of
the Reaction Setup screen. For this field, enter the sample concentration you want to use
to add to the reaction mix for all samples in the experiment. "10✕ for Reaction Mix"
indicates that the software assumes the sample or standard component of the reaction mix
is at a 10✕ concentration. For example, if the diluted sample concentration is 50.0 ng/µL
(10✕), the final sample concentration in the reaction is 5 ng/µL (1✕).
See serial factor.
See melt curve.
See amplification efficiency
A target or gene that should be expressed at similar levels in all samples you are testing.
Endogenous controls are used in relative standard curve and comparative C
experiments to normalize fluorescence signals for the target you are quantifying.
Housekeeping genes can be used as endogenous controls. See also
See post-PCR read.
Applied Biosystems StepOne
(∆Rn).
software that helps you set up your experiment by guiding you
(EFF%).
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and StepOnePlus
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
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∆∆
( C )
T T
housekeeping gene.
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Real-Time PCR Systems