Setting The Volume - Labnet BioPette PLUS BPP2 Instuction Manual

The accuracy and precision (repeatability) of liquid vol-
ume depend on the quality of tips used. The values for
accuracy and precision given in the table below were
obtained using Labnet tips. Only those tips guarantee
correct operation of the pipettes and ensure accuracy
and precision of liquid sampling.
Volume Accuracy
Model
[μl]
0.2
BPP2
1.0
Max 2.0
Min 0.5
BPP10
5.0
Max 10.0
Min 2
BPP20
10
Max 20
Min 10
BPP100
50
Max 100
Min 20
BPP200
100
Max 200
Min 100
BPP1000
500
Max 1000
Min 1000
BPP5000
2500
Max 5000
Min 1000
BPP10000
5000
Max 10000
These specifications are obtained in forward mode,
using a gravimetric method with the temperature of the
distilled water, tips and all other conditions stabilized
between 19°C and 21°C. Number of measurements -
minimum 10. The values given include all components of
error due to both normal handwarming and the changing
of the tip.
Performance test: The pipette is calibrated in accordance
with EN ISO 8655. Performance can be verified by
checking the pipette using the procedures outlined in the
EN ISO 8655 standard.
The pipette design enables the user to perform the recalibra-
tion process according to the rules presented in section 8.
3
Precision Fit to tips
[%] [%] μl
± 12.0 ± 6.0
± 2.7 ± 1.3
± 1.5 ± 0.7
10
± 4.0 ± 2.8
± 1.0 ± 0.6
± 0.5 ± 0.4
± 3.0 ± 1.5
± 1.0 ± 0.5
± 0.8 ± 0.3
± 1.6 ± 0.80
200
± 0.8 ± 0.24
± 0.8 ± 0.20
± 1.2 ± 0.60
± 0.8 ± 0.25
± 0.6 ± 0.20
± 1.6 ± 0.40
1000
± 0.7 ± 0.20
± 0.6 ± 0.15
± 0.6 ± 0.25
5000
± 0.6 ± 0.20
± 0.5 ± 0.15
± 2.5 ± 0.6
10000
± 0.8 ± 0.3
± 0.5 ± 0.2

2 - SETTING THE VOLUME

The volume display shown by the counter has three dig-
its, which should be read from top to bottom. In addition
the lowest counter drum is printed in a scale which
enables the setting of the volume in the minimum gradua-
tion range. Examples of the meanings of the black and
red digits:
Pipettes BPP2
Red figures at the bottom = 1/100 μl
Increment = 0.002 μl
Pipettes BPP10, BPP20,
Red figures at the bottom = 1/10 μl
Increment = 0.02 μl
Pipettes BBPP100, BPP200
Black figures only = 1 μl
Increment = 0.2 μl
Pipettes BPP1000, BPP5000,
Red figures at the top - ml
Increment =
Pipettes BPP10000
Red figures at the top - ml
Increment = 20 μl
The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the adjustment knob
(Fig. 1B). The volume adjustment can be performed
when the locking ring is set in the lower position (fig. 5A).
When the desired volume is selected, the the locking ring
should be set in the upper position (Fig.5B). To attain the
maximum accuracy, set volume must be approached
from a higher value by diminishing counter readings.
• If the desired volume is lower than set volume shown
by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the adjustment knob (Fig. 1B)
BPP2
BPP10 BPP20
BPP100 BPP200
BPP1000 BPP5000
2 μl 10 μl
BPP10000
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