Related Manuals for Labnet ENDURO GEL XL E0160
Summary of Contents for Labnet ENDURO GEL XL E0160
- Page 1 ENDURO™ GEL XL User Manual HORIZONTAL GEL ELECTROPHORESIS UNIT E0160 E0160-230V E0160-230V-UK E160-CAN Lit M00078 Rev 3; September 2016...
- Page 2 About This Manual This manual is designed to assist you in the optimal usage of your Enduro GelXL. The manual is available in English, French, German, Italian, Portuguese, and Spanish on our website at www.labnetinternational.com/document-center...
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Page 3: Table Of Contents
TABLE OF CONTENTS MAINTENANCE OPTIONS AND SPECIFICATIONS A. Components and Accessories B. Specifications III. OPERATING INSTRUCTIONS A. Preparation of the Agarose Gel and Electrophoresis Buffer – DNA B. Preparation of the Agarose Gel and Electrophoresis Buffer - RNA C. Casting the Gel D. -
Page 4: Maintenance
MAINTENANCE Please handle the unit with care: Do not expose the unit or its accessories to temperatures above 60°C. Do not expose the unit to organic solvents. Do not clean the unit with abrasive cleaners or cleaning aids. In most cases, rinsing with deionized water will sufficiently clean the unit. For heavier dirt, use a mild cleansing solution such as dish soap (alkaline cleansers are not recommended). -
Page 5: Options And Specifications
WARNINGS: CAUTION! Injury, damage to equipment, or property, may result if used in a manner not specified by the manufacturer. CAUTION! A pinch hazard exists between the plastic enclosure and the shaking head. CAUTION! NOT for use with flammable liquids. OPTIONS AND SPECIFICATIONS Components and Accessories Catalog #... -
Page 6: Operating Instructions
Power Supply Overall dimension 7.5 × 17.0 × 6.2 cm Weight 410 g Input Voltage AC100 - 240V, 50/60Hz Output Voltage 10 to 150 volts; Constant peak voltage of 150V Output Amperage 10 to 400 mA Maximum Wattage 45 W Timer 99 hours 59 min, and continuous model... - Page 7 resolving powers of these buffers are very similar, the relative buffer capacities are very different, conferring different run attributes which are summarized below: TAE: Tris-acetate has traditionally been the more commonly used buffer. However, its relatively low buffer capacity will become exhausted during extended electrophoresis, making buffer recirculation necessary in runs exceeding 140 mA-hours.
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Page 8: Preparation Of The Agarose Gel And Electrophoresis Buffer - Rna
8. Add deionized water to replace any volume lost through evaporation during the heating process. Proceed to Section C, Step 1, “Casting the Gel” on page 12. Preparation of the Agarose Gel and Electrophoresis Buffer - RNA RNA molecules are separated by electrophoresis through denaturing gels prior to (1, 2, 3) analysis by northern hybridization. -
Page 9: Casting The Gel
6. Allow the solution to cool to 60 C. Place the flask in a hood and add 5 ml of 10X MAE buffer (see Appendix A for recipe), and 1.5 ml of 37 % formaldehyde. CAUTION: Formaldehyde vapors are toxic. Gel preparation should take place in a hood and solutions and gels containing formaldehyde should be kept covered when possible. -
Page 10: Removing The Comb
comb to either pop them or lightly brush them to the sides of the gel. If large bubbles are allowed to harden within the gel, they may cause artifacts to occur during electrophoresis. 5. Insert one or more combs by placing them into the slots in the casting stand. -
Page 11: Loading The Samples Onto The Gel
samples. 4. Load the samples into the wells with a micropipette or similar device taking care not to puncture the bottom of the wells or load the sample onto the top of the gel. . Electrical Connections to the Safety Lid and The ENDURO Gel XL can only be operated with the safety lid in place. -
Page 12: Sample Electrophoresis
Sample Electrophoresis The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the Gel XL is 150 volts. In a 1 % TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, and as a general rule, a 70 volt run will take twice as long as a 140 V run. - Page 13 them to medium wavelength (312 nm) UV light. Because the gel casting tray is UV transmittant, the gel does not need to be removed from the tray before viewing. Place the gel casting tray containing the gel on the filter surface of a UV Transilluminator for convenient viewing.
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Page 14: Iv. Appendices
IV. APPENDICES Buffers for Electrophoresis Tris Acetate EDTA Buffer (TAE): 1X Working Concentration: 10X Stock Solution: 40 mM Tris base 48.4 g Tris Base 20 mM Glacial Acetic Acid (NaOAc) 16.4 g or 11.42 ml NaOAc 2.0 mM EDTA pH 8.3 7.4 g EDTA or 20 ml 0.5 M EDTA (pH 8.0) -
Page 15: Physical Properties Of Electrophoretic Plastics
Solutions containing MOPS should be wrapped in aluminum foil and stored at room temperature. The buffer tends to yellow with age. Light yellow buffer may be used, however, dark yellow solutions should be discarded. Sample Loading Buffer, DNA Sample Loading Buffer, RNA 10X Stock Solution: 5X Stock Solution: 50 % Glycerol... - Page 16 For Technical Support and Information in the USA: Labnet International 31 Mayfield Ave. Edison, NJ 08837 Tel: 732-417-0700 www.labnetinternational.com Symbols and Conventions The following chart is an illustrated glossary of the symbols that may be used in this manual or on the product.
- Page 17 EQUIPMENT DISPOSAL-EUROPEAN REGULATIONS According to Directive 2012/19/EU of the European Parliament and of the Council of 4 July 2012 on waste electrical and electronic equipment (WEEE), Enduro GelXL is marked with the crossed-out wheeled bin and must not be disposed of with domestic waste. Consequently, the buyer shall follow the instructions for reuse and recycling of waste electronic and electrical equipment (WEEE) provided with the...
- Page 18 NOTES...
- Page 19 31 Mayfield Ave. Edison, NJ 08837 P: +732-417-0700 F: +732-417-1750 Toll Free: 888-522-6381 labnetinfo@corning.com www.labnetinternational.com 9300130000...
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