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SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
5
Grasp the bucket cap tabs and lower the buckets into the openings on the rotor body with the
rounded sides toward the outside of the rotor.
NOTE
A bucket will not seat in the rotor if it is inserted in the rotor with the rounded side toward the rotor
center. The bucket will fall through, spilling or disturbing the tube contents.
Six bucket assemblies must be installed, whether loaded or empty. If fewer than six tubes are being run,
they must be arranged symmetrically in the rotor (see Figure 1). Opposing tubes must be filled to
the same level with liquid of the same density.
Figure 1 Arranging Tubes Symmetrically in the Rotor Buckets
NOTE
Two, three, four, or six tubes can be centrifuged per run if they are arranged in the rotor as shown. All
bucket assemblies must be attached to the rotor, whether loaded or empty.
Operation
For low-temperature runs, precool the rotor in the centrifuge or in a refrigerator before use—especially
before short runs—to ensure that the rotor reaches the set temperature. A suggested precooling cycle is a
minimum of 30 minutes at 2000 RPM at the required temperature.
1
Refer to the centrifuge instruction manual for additional operating instructions.
2
For additional operating information, see the following:
•
Run
•
Run
•
Selecting CsCl
centrifugation.
6
Times, page 12, for using k factors to adjust run durations.
Speeds, page 13, for information about speed limitations.
Gradients, page 19, for methods to avoid CsCl precipitation during
LXL-TB-015BB
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