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Summary of Contents for Beckman Coulter SW 32 Ti
- Page 1 Instructions For Use SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors For Use in Beckman Coulter Class H, R, and S Preparative Ultracentrifuges LXL-TB-015BB November 2011 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
- Page 2 SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors LXL-TB-015AE (November 2011) Copyright © 2011 Beckman Coulter, Inc. All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.
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Page 3: Safety Notice
These rotors were developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge/ rotor system. Their safety or reliability cannot be assured if used in an ultracentrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter ultracentrifuge that has been modified... - Page 4 Although rotor components and accessories made by other manufacturers may fit in the SW 32 Ti and SW 32.1 Ti rotors, their safety in these rotors cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in these rotors may void the rotor warranty and should be prohibited by your laboratory safety officer.
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Page 5: Table Of Contents
Safety Notice, iii Alerts for Danger, Warning, Caution, and Note, iii Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors, iii SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors, 1 Specifications for the SW 32 Ti Rotor, 1 Specifications for the SW 32.1 Ti... - Page 7 Arranging Tubes Symmetrically in the Rotor Buckets, 6 Precipitation Curves for the SW 32 Ti Rotor.r, 15 CsCl Gradients at Equilibrium for the SW 32 Ti Rotor, 16 Precipitation Curves for the SW 32.1 Ti Rotor, 17 CsCl Gradients at Equilibrium for the SW 32.1 Ti...
- Page 8 Tables Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotor, 8 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti Rotor, 9 Relative Centrifugal Fields for the SW 32 Ti and SW 32.1 Ti Rotors, 14 viii...
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Page 9: Sw 32 Ti And Sw 32.1 Ti Swinging-Bucket Rotors
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Specifications for the SW 32 Ti Rotor Maximum speed ......... . . 32,000 RPM Density rating at maximum speed. -
Page 10: Specifications For The Sw 32.1 Ti Rotor
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Specifications for the SW 32.1 Ti Rotor Specifications for the SW 32.1 Ti Rotor Maximum speed ......... . . 32,000 RPM Density rating at maximum speed. -
Page 11: Description
These Beckman Coulter rotors have been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter ultracentrifuges. The SW 32 Ti and SW 32.1 Ti are swinging bucket rotors designed to centrifuge up to six tubes each. Used in Beckman Coulter class H, R, and S preparative ultracentrifuges, these rotors develop centrifugal forces for the separation of subcellular particles and viruses in density gradients. -
Page 12: Preparation And Use
Preparation and Use Preparation and Use Specific information about the SW 32 Ti and SW 32.1 Ti rotors is given here. Information common to these and other rotors is contained in Rotors and Tubes for Preparative Ultracentrifuges (publication LR-IM), which should be used together with this manual for complete rotor and accessory operation. Publication LR-IM is included in the literature package with this rotor manual. - Page 13 8. SW 32.1 Ti Bucket CAUTION Use only black caps (369643) on black SW 32 Ti buckets and red caps (369645) on red SW 32.1 Ti buckets. Use a lint-free cotton swab to apply Spinkote™ lubricant (396812) to cap grooves in the bucket tops.
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Page 14: Operation
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Grasp the bucket cap tabs and lower the buckets into the openings on the rotor body with the rounded sides toward the outside of the rotor. NOTE A bucket will not seat in the rotor if it is inserted in the rotor with the rounded side toward the rotor center. -
Page 15: Removal And Sample Recovery
To remove the rotor from the centrifuge, lift the rotor straight up and off the drive hub. a. Set the rotor on the rotor stand. Tubes and Accessories The SW 32 Ti rotor uses tubes and accessories listed in Table 1; the SW 32.1 Ti rotor uses tubes and... - Page 16 SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Table 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotor Tube Required Accessory Dimensions/ Max Speed/ Nominal Part Part RCF/ Volume/ Description Number Description Number k factor 25 ...
- Page 17 SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Table 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotor (Continued) Tube Required Accessory Dimensions/ Max Speed/ Nominal Part Part RCF/ Volume/ Description Number Description...
- Page 18 SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Table 2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti Rotor (Continued) Tube Required Accessory Dimensions/ Max Speed/ Nominal Part Part RCF/ Volume/ Description Number Description...
- Page 19 Quick-Seal tubes and floating spacers (also called g-Max spacers). • This means that you can run the shorter tubes listed in the Tables in the SW 32 Ti and SW 32.1 T rotors without reduction in g force.
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Page 20: Run Times
Run Times The k factor of the rotor is a measure of the rotor’s pelleting efficiency. (Beckman Coulter has calculated the k factors for all of its preparative rotors at maximum rated speed and using full tubes.) The k factor is calculated from the formula ... -
Page 21: Run Speeds
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Use the k factor in the following equation to estimate the run time t (in hours) required to pellet particles of known sedimentation coefficient s (in Svedberg units, S). - Page 22 SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Table 3 Relative Centrifugal Fields for the SW 32 Ti and SW 32.1 Ti Rotors Relative Centrifugal Field (g) Relative Centrifugal Field (g) Rotor Rotor At r At r...
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Page 23: Precipitation Curves For The Sw 32 Ti
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Figure 2 Precipitation Curves for the SW 32 Ti Rotor.r 1.90 1.80 1.70 1.60 1.50 1.40 1.30 SW 32 Ti ROTOR = 20°C = 4°C 1.20 1.10 5000... -
Page 24: Cscl Gradients At Equilibrium For The Sw 32 Ti
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Figure 3 CsCl Gradients at Equilibrium for the SW 32 Ti Rotor 1.90 1.80 1.70 SW 32 Ti ROTOR 1.60 = 20°C = 4°C 1.50 1.40 1.30 1.20 1.10... -
Page 25: Precipitation Curves For The Sw 32.1 Ti
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Figure 4 Precipitation Curves for the SW 32.1 Ti Rotor 1.90 1.80 1.70 1.60 1.50 1.40 1.30 SW 32.1 Ti ROTOR = 20°C = 4°C 1.20 1.10 5000... -
Page 26: Cscl Gradients At Equilibrium For The Sw 32.1 Ti
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Preparation and Use Figure 5 CsCl Gradients at Equilibrium for the SW 32.1 Ti 1.90 1.80 1.70 SW 32.1 Ti ROTOR = 20°C 1.60 = 4°C 1.50 1.40 1.30 1.20 1.10 1.00... -
Page 27: Selecting Cscl Gradients
Partial filling may be desirable when there is little sample or when you wish to shorten the run time For example, in the SW 32 Ti rotor, a quarter-filled tube of 1.69-g/mL homogeneous CsCl solution at 4°C may be centrifuged at 25,000 RPM (see Figure 2). -
Page 28: Typical Examples For Determining Cscl Run Parameters
Typical Examples for Determining CsCl Run Parameters Example A: Starting with a homogeneous CsCl solution density of 1.40 g/mL and approximate particle buoyant densities of 1.38 and 1.42 g/mL, at 20°C, where will particles band at equilibrium in the SW 32 Ti rotor? 1. At Speed 2. -
Page 29: Care And Maintenance
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Care and Maintenance Select the curve at the desired temperature (20°C) and tube volume (full) that gives the best particle separation. Note the run speed along the selected curve (20,000 RPM). -
Page 30: Cleaning
(306812). Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and accessory materials. • Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. Cleaning Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. -
Page 31: Decontamination
Use the minimum immersion time for each solution, per laboratory standards. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 32: Supply List
NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com, by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. See the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (BR-8101, available at www.beckmancoulter.com) or contact Beckman Coulter Sales (1-800-742-2345 in the United States) -
Page 33: Other
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Supply List Other NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com. Description Part Number Tubes and accessories Table 1 Table 2 Bucket holder rack 331186 Quick-Seal Cordless Tube Topper kit, 60 Hz... - Page 34 SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors Supply List LXL-TB-015BB...
- Page 35 2. If a Beckman Coulter centrifuge is damaged due to a failure of a rotor covered by this warranty, Beckman Coulter will supply free of charge (i) all centrifuge parts required for repair (except the drive unit, which...
- Page 36 Beckman Coulter will repair or replace the rotor free of charge. 4. If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube, bottle, tube cap, spacer, or adapter, covered under the Conditions of this Warranty, Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point (2) above, and the replacement policy.
- Page 38 Related Documents Rotors and Tubes for Preparative Additional References Ultracentrifuges (LR-IM) • Chemical Resistances for Beckman Coulter Centrifugation Products (IN-175) • Rotors • Beckman Coulter Ultracentrifuge Rotors, • Tubes, Bottles, and Accessories Tubes & Accessories catalog (BR-8101) • Using Tubes, Bottles, and Accessories •...
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