Table of Contents
Advertisement
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
3
Refer to Rotors and Tubes for detailed information on the use and care of Quick-Seal tubes.
• Quick-Seal tubes are disposable and should be discarded after a single use.
konical™ Tubes
Polyallomer konical tubes, used to optimize pelleting separations, have a conical tip that
concentrates the pellet in the narrow end of the tube. The narrow bottom also reduces the tube's
nominal volume and minimizes gradient material requirement. The konical tubes come in both
open-top and Quick-Seal tube designs. Conical cavity adapters hold the tubes in the rotor buckets
Polyallomer and Ultra-Clear Open-Top Tubes
Polyallomer and Ultra-Clear® open-top tubes should be filled as full as possible (2 or 3 mm from the
tube top) for tube support. If necessary, float mineral oil (or some other low-density, immiscible
liquid) on top of the tube contents to fill the tube to its maximum volume. (Do not use an oil overlay
in Ultra-Clear tubes.) All opposing tubes for a run must be filled to the same level with liquid of the
same density.
Run Times
The k factor of the rotor is a measure of the rotor's pelleting efficiency. (Beckman Coulter
has calculated the k factors for all of its preparative rotors at maximum rated speed and using
full tubes.) The k factor is calculated from the formula
where is the angular velocity of the rotor in radians per second ( = 0.105 RPM), r
maximum radius, and r
After substitution:
12
1
ln
r
max
k
=
---------------------------------- -
is the minimum radius.
min
2.533
10
k
=
----------------------------------------------------------------------- -
1. Adapters
13
r
10
min
----------- -
3600
2
11
r
ln
r
min
max
2
RPM
EQ 1
is the
max
EQ 2
LXL-TB-015BB
Hide quick links:
Advertisement
Table of Contents
