Beckman Coulter Spinchron DLX Instruction Manual
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Beckman Coulter Spinchron DLX Instruction Manual

Benchtop centrifuge
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SDLX-IM-8
Spinchron
DLX Benchtop Centrifuge
™
Instruction Manual

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Summary of Contents for Beckman Coulter Spinchron DLX

  • Page 1 SDLX-IM-8 Spinchron DLX Benchtop Centrifuge ™ Instruction Manual...
  • Page 2 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 3 SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual. The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation, operation, maintenance, or repair of this centri- fuge.
  • Page 4 Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter Field Service.
  • Page 5 SDLX-IM-8 December 2007 Spinchron DLX Benchtop Centrifuge ™ Instruction Manual © 2007–2009 Beckman Coulter, Inc.

  • Page 7: Table Of Contents

    Contents Page INTRODUCTION Certification ..........Scope of Manual.
  • Page 8 Contents Page SECTION 3: OPERATION Run Preparation ......... . 3-2 Setting Run Parameters .
  • Page 9 Chamber ..........1-4 Figure 2-1. Dimensions of the Spinchron DLX Centrifuge ....2-2 Tables Page Table 3-1.

  • Page 11: Introduction

    SCOPE OF MANUAL This manual is designed to familiarize you with the Beckman Coulter Spinchron DLX centrifuge, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you...

  • Page 12: Conventions

    Further, the use of any equipment other than that recom- mended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

  • Page 13: Cfc-Free Centrifugation

    • Cursor keys, used to increment values up or down when setting parameters, are shown as up and down arrows (▲ and ▼). CFC-FREE CENTRIFUGATION To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Spinchron DLX centrifuges.

  • Page 14: Radio Interference

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment,...

  • Page 15: Description

    Description CENTRIFUGE FUNCTION, SPECIFICATIONS, AND SAFETY FEATURES CENTRIFUGE FUNCTION The Beckman Coulter Spinchron DLX is a nonrefrigerated compact benchtop centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include: •...

  • Page 16: Specifications

    * Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected. SAFETY FEATURES The Spinchron DLX centrifuge has been designed and tested to operate safely indoors at altitudes up to 2 000 m (6 562 ft). Safety features include the following: •...

  • Page 17: Centrifuge Chassis

    Description rotors. When the door is closed it locks automatically. It can be unlocked only by pressing the [ ] key, and opened only OPEN DOOR when the power is on, the rotor is at rest, and the manual lock is in the unlocked position.

  • Page 18: Rotor Chamber

    Holes in the side of the bowl (4) allow air to flow out to the exhaust ports beneath the centrifuge. This air flow provides cooling for the rotor. Figure 1-1. Interior View of the Spinchron DLX Centrifuge Rotor Chamber DRIVE The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation.

  • Page 19: Control Panel

    Description selected to reduce deceleration time, allowing fast processing of samples; alternately, delicate gradients may be preserved using slower deceleration. CONTROL PANEL Mounted at an angle on the centrifuge front for easy visibility and access, the control panel comprises touch keys—system keys and parameter keys—and digital displays.

  • Page 20: Parameter Keys

    Description When pressed, the centrifuge decelerates to a complete stop STOP according to the selected deceleration rate, deceleration can be terminated and the centrifuge restarted by pressing [ ] again. START Causes a timed or continuous run to begin; can also be used to abort START deceleration and restart the centrifuge.

  • Page 21: Digital Displays

    Description Selects the run duration. TIME Timed run — can be set up to 9 hours and 59 minutes; a minutes • parameter exceeding 59 is automatically converted into hours. Continuous run — continuous operation is activated if a run time •...
  • Page 22 Description SPEED The contents of this display vary, depending on the operating mode. • In input mode, displays the value of the parameter being set ], [ ], or [ ]). For example, if the [ ] parameter ROTOR ROTOR key is pressed, a rotor number appears on the SPEED display.

  • Page 23: Available Rotors

    Description AVAILABLE ROTORS Refer to the applicable rotor manuals for complete rotor descriptions. Rotor Manual Rotor Profile and Description (× g) Capacity Number GH-3.8A Swinging Bucket (4 place) GS6-TB-017 4 × 750 mL Buckets (r = 204 mm) 3750 3210 4 ×...
  • Page 24 Description Rotor Manual Rotor Profile and Description (× g) Capacity Number PTS-2000 Horizontal (4 place) SP-TB-008 7 × 10 mL Sector base, 100-mm tube 3250 2010 = 170 mm) (each carrier) 7 × 7 mL Sector base, 75-mm tube 3250 1890 = 160 mm) (each carrier)

  • Page 25: Installation Requirements

    Installation Requirements WARNING This centrifuge weighs 65.4 kg (144.0 lb). DO NOT attempt to lift or move it without assistance from another person. INSTALLATION REQUIREMENTS WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge’s air system and be ignited.

  • Page 26: Space And Location Requirements

    The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation. Dimensions are shown in Figure 2-1. 94.0 37.0 39.4 15.5 58.4 23.0 54.6 21.5 Figure 2-1. Dimensions of the Spinchron DLX Centrifuge...

  • Page 27: Electrical Requirements

    NOTE In regions where a different plug is required to meet local electrical and safety requirements, contact your local Beckman Coulter representative. Make sure the voltage imprinted on the name rating plate affixed to the back of the centrifuge agrees with the line voltage of the outlet used.

  • Page 28: Test Run

    NAME RATING PLATE When corresponding with Beckman Coulter regarding your Spinchron DLX centrifuge, always mention its model and serial number. The serial number is shown on the name rating plate affixed to the rear of the centrifuge, and is also on the inside of the door.
  • Page 29 Operation This section contains operating procedures for the Spinchron DLX centrifuge, using any of the rotors designed for use in this centrifuge. A summary is provided at the end of this section. If you are an experi- enced user of this centrifuge, you can turn to the summary for a quick review of operating steps.

  • Page 30: Operation

    Operation RUN PREPARATION The following detailed operating procedures are summarized at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation.

  • Page 31: Setting Run Parameters

    Operation Action Result 6. Load the rotor according to the rotor manual. WARNING Do not substitute a metal fastener for the plastic tie-down nut supplied with the rotor. If a tie-down nut should come off the shaft during centrifugation, the plastic nut furnished will break apart in the chamber, causing minimal damage to the centrifuge.

  • Page 32: Selecting A Rotor

    Operation SELECTING A ROTOR Make sure the rotor is seated on the drive shaft and the tie-down nut is tight before each run. Action Result 1. Press [ The number of the rotor used in the previous run appears. ROTOR 2.

  • Page 33: Setting Run Time

    Operation Setting RPM Action Result SPEED 1. Press [ The last digit on the SPEED display flashes. 2. Press ▲ or ▼ to scroll The corresponding RCF will be automatically calculated by the through speeds until the centrifuge. required speed appears. Setting RCF Action Result...

  • Page 34: Setting Acceleration And Deceleration Rates

    Operation SETTING ACCELERATION AND DECELERATION RATES You can select one of nine acceleration and nine deceleration rates that will maintain optimum separation of delicate samples. Select rates depending on the type of run you are performing—for pelleting runs, where sample mixing is not a concern, maximum brake can be used.

  • Page 35: Starting A Run

    Operation Setting a Deceleration Rate Action Result ACC/DEC 1. Press [ The ACC/DEC display flashes. DECEL 2. Press ▲ or ▼ until the selected rate is displayed. STARTING A RUN ➠ NOTE For a short-duration run, press [ ] and then ENTER press the [ ] key.

  • Page 36: Changing Parameters During A Run

    Operation Action Result 1. Check that all parameters are correct and the door is CAUTION closed. Do not lift or move the centrifuge while the rotor is spinning. 2. Move the manual lock lever to the right (locked position). 3. Press [ ], then press •...

  • Page 37: Stopping A Run

    Operation ➠ NOTE The deceleration rate cannot be changed after deceleration starts. Use the parameter keys as described under ENTERING RUN , above, to change parameters. You must press [ PARAMETERS ENTER after you input each change. For example, you would change run speed during centrifugation as follows: Action Result...

  • Page 38: Unloading

    Operation Action Result 2. After the rotor stops spinning and the [ ] light OPEN DOOR CAUTION comes on, move the manual The door interlock system will release the latch lock lever to the left and allow the door to be opened when the rotor (unlocked).

  • Page 39: Summary Of Run Procedures

    Operation SUMMARY OF RUN PROCEDURES 1. Press the power switch to on (I). 2. Move the manual lock lever to the left (unlocked), then press ] and lift the door up. OPEN DOOR 3. Install the rotor according to the applicable rotor manual. Always run the rotor with a balanced load.

  • Page 41: Troubleshooting

    Beckman Coulter Field Service. ➠ NOTE It is your responsibility to decontaminate the centrifuge, as well as any accessories, before requesting service by Beckman Coulter Field Service representatives. USER MESSAGES CAUTION If the message SEr appears on the display, do not press any keys while the message is displayed.
  • Page 42 Troubleshooting Table 4-1. Error Message Chart. If the recommended action does not correct the problem, notify Beckman Coulter Field Service. Error Number Problem Result Recommended Action 1 through 22, Microprocessor or Deceleration to full stop; After rotor comes to a complete stop, turn...

  • Page 43: Other Possible Problems

    Possible causes for each problem are listed in the probable order of occurrence. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service. Table 4-2. Troubleshooting Chart Problem...

  • Page 44: Accessing The Rotor In Case Of Power Failure

    Troubleshooting ACCESSING THE ROTOR IN CASE OF POWER FAILURE If the facility power fails only momentarily, the centrifuge will resume operation when power is restored and the rotor will return to set speed. However, if the rotor comes to a complete stop you will have to restart the run when the power is restored.
  • Page 45 Troubleshooting Action Result 4. Insert the supplied hex wrench (929317) into the override key slot in the left Secondary (manual) Lock Lever side panel. 5. Turn the hex wrench (either The latch “clicks” or the door pops open. direction). If the rotor is still spinning, close the door and wait until it stops before attempting to remove it.

  • Page 47: Care And Maintenance

    Care and Maintenance For maintenance not covered in this manual, contact Beckman Coulter Field Service. WARNING Any maintenance procedure or servicing of this equipment that requires removal of any covers can expose parts that involve the risk of electric shock or personal injury. Make sure that the power switch is off (O) and the centrifuge is disconnected from the main power source, and refer such servicing to...

  • Page 48: Cleaning

    Care and Maintenance • To prevent the rotor from sticking, lubricate the drive shaft with Spinkote (306812) at least once a month, and after each cleaning. • Refer to the applicable rotor manual for instructions on the care of rotors and other components and accessories. ➠...

  • Page 49: Decontamination

    While Beckman Coulter has tested this method and found that it does not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 50: Storage And Transport

    Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions.

  • Page 51: Supply List

    742-2345 in the United States, or by contacting your local Beckman Coulter office. Contact Beckman Coulter Sales (1-800-742-2345 in the United States; worldwide offices are listed at the back of this manual) for information about ordering parts and supplies. For your convenience, a partial list is given below.
  • Page 53 CONDITIONS below and the warranty clause of the Beckman Coulter terms and conditions in effect at the time of sale, Beckman Coulter Beckman Coulter makes no warranty concerning products or agrees to correct either by repair or, at its election, by replace- accessories not manufactured by it.
  • Page 55 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com ©2009 Beckman Coulter, Inc. All rights reserved...

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