Beckman Coulter SX4750A Instruction For Use, Installation And Assembly
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Beckman Coulter SX4750A Instruction For Use, Installation And Assembly

Swinging-bucket rotor
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Instructions For Use
SX4750A
Swinging-Bucket Rotor
For Use in the Beckman Coulter
Allegra X-12 Series,
Allegra X-14 Series, and
Allegra X-15R Centrifuges
GX-TB-004EE
February 2018
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

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  • Page 1 Instructions For Use SX4750A Swinging-Bucket Rotor For Use in the Beckman Coulter Allegra X-12 Series, Allegra X-14 Series, and Allegra X-15R Centrifuges GX-TB-004EE February 2018 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
  • Page 2 GX-TB-004EE (February 2018) © 2018 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

  • Page 3: Revision History

    • Replacement Rotor Parts Issue EC, 8/13 The following sections have changed subsequent to revision EB: • Table 2, Beckman Coulter Tubes and Bottles for the SX4750A Rotor • Beckman Coulter, Inc. SX4750A Warranty Issue ED, 11/15 The following sections have changed subsequent to revision EC: •...
  • Page 4 Revision History GX-TB-004EE...

  • Page 5: Safety Notice

    World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a GX-TB-004EE...
  • Page 6 Although rotor components and accessories made by other manufacturers may fit in the SX4750A rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the SX4750A rotor may void the rotor warranty and should be prohibited by your laboratory safety officer.

  • Page 7: Table Of Contents

    Contents Revision History, iii Safety Notice, v Alerts for Warning, Caution, and Note, v Safety Information for the SX4750A Rotor, v SX4750A Swinging-Bucket Rotor, 1 Specifications, 1 Description, 2 Buckets and Accessories, 3 Modular Disk Adapters, 3 Bottle Adapters, 3...
  • Page 8 Adapters, 27 Decontamination, 27 Sterilization and Disinfection, 28 Tube Breakage, 28 Aerosolve Cannisters, 29 SST or Corvac Tubes, 29 Storage, 30 Returning a Rotor, 30 Supply List, 31 Replacement Rotor Parts, 31 Other, 31 Beckman Coulter, Inc. SX4750A Warranty viii...
  • Page 9 Illustrations Illustrations Examples of Correctly and Incorrectly Loaded Buckets and Carriers, 9 Assembling a Modular Disk Adapter, 10 Blood Bag Loading Procedures, 13 Lubricating Pivot Pin/Bucket Contact Areas, 26...
  • Page 10 Tables Tables Adapters and Blood Bag Cups Available for the SX4750A Rotor, 18 Beckman Coulter Tubes and Bottles for the SX4750A Rotor, 20 Beckman Coulter Labware for Use with Multiwell-Plate Carriers, 23 Commercially Available Labware for Use with Multiwell-Plate Carriers, 24...

  • Page 11: Sx4750A

    SX4750A Swinging-Bucket Rotor Specifications Maximum speed Allegra X-15R (tube-and-bottle buckets) ......4750 RPM Allegra X-15R (multiwell-plate carriers)....... 4450 RPM Allegra X-14 series (tube-and-bottle buckets) ..... 4300 RPM Allegra X-14 series (multiwell-plate carriers)...... 4000 RPM Allegra X-12 series (all buckets) ..........3750 RPM Critical speed range ............580 to 720 RPM...

  • Page 12: Description

    5. Carriage 3. Bottle This Beckman Coulter rotor has been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges. The SX4750A is a four-place swinging bucket rotor. The rotor buckets carry adapters that allow centrifugation of a wide range of tube and bottle sizes (from 1.5 mL reaction vials to 750-mL bottles)

  • Page 13: Buckets And Accessories

    SX4750A Swinging-Bucket Rotor Description Buckets and Accessories Several types of labware can be run in the buckets, depending on your application. Modular Disk Adapters Tubes are supported in modular disk adapters, which can also serve as tube racks in the laboratory.

  • Page 14: Aerosolve Cannisters

    3. Aerosolve Cannister Aerosolve cannisters, designed to contain aerosol leakage and liquid spills, can be used in the SX4750A buckets when this additional containment is required. The cannister was tested demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed.

  • Page 15: Multiwell-Plate Carriages

    Run Preparation Specific information about the SX4750A rotor is given here. Information about the use and care of the centrifuge is contained in the centrifuge manual, which should be used together with this manual for complete rotor and centrifuge operation.

  • Page 16: Installing The Rotor Yoke

    SX4750A Swinging-Bucket Rotor Run Preparation Installing the Rotor Yoke NOTE Before the first use of a new rotor, attach the tie-down T-bar (369718) to the rotor yoke. Screw it down as far as possible. Before installing the yoke in the centrifuge, lightly lubricate the drive hole with a lubricant such as Spinkote (306812).

  • Page 17: Loading The Buckets

    Symmetrical and Balanced Loading Beckman Coulter supplies buckets and carriers in weight-matched sets to make balancing easier (the weight and retirement date are marked on the side of each bucket or carrier). Modular disk adapters are also sold in weight-matched sets.

  • Page 18: Using Tube And Bottle Buckets

    SX4750A Swinging-Bucket Rotor Loading the Buckets It is not necessary to completely fill all tubes or positions in buckets; however, partially filled buckets must be balanced with respect to the bucket pivotal axis (see Figure 1). In multitube adapters, each tube should be placed so that its weight is balanced by a tube in a diametrically opposite position across the pivotal axis in the same adapter.
  • Page 19 SX4750A Swinging-Bucket Rotor Loading the Buckets Figure 1 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Balanced Load Unbalanced Load CORRECT INCORRECT 1. Balanced Load 5. CORRECT 2. Unbalanced Load 6. Examples of Symmetrically Loaded Multiwell Plates (load opposite plates the same way) 3.

  • Page 20: Using Tubes

    SX4750A Swinging-Bucket Rotor Loading the Buckets CAUTION Buckets are designed to be used only with modular disk adapters, bottle adapters, cell culture flask adapters, Aerosolve cannisters, and blood bag cups. Do not pour samples directly into buckets, bottle sleeves, or blood bag cups. Do not load bottles, cell culture flasks, or tubes directly into the buckets.

  • Page 21: Loading Tubes In Adapters

    SX4750A Swinging-Bucket Rotor Loading the Buckets Add more disks in this way until the height of the assembly is nearly as tall as the tubes you will be using. • (If the height of the disks is very tall, you may have to push the bail into the grooves of the top disks by hand.)

  • Page 22: Using Aerosolve Cannisters

    Aerosolve tube racks and the number and sizes of tubes they accommodate. When Aerosolve cannisters are used in the SX4750A rotor buckets, pads (361269) must be placed beneath the cannisters in the buckets to provide support and prevent the cannisters from being damaged during centrifugation.
  • Page 23 SX4750A Swinging-Bucket Rotor Loading the Buckets Figure 3 Blood Bag Loading Procedures 1. Blood Bag Label 4. Blood Bag Seam 2. Blood Bag Seam Aligned with 5. Blood Bag Cup Pivotal Axis of Bucket 6. Sandwich Coiled Tubing between 3. Ports...

  • Page 24: Using Cell Culture Flask Adapters

    SX4750A Swinging-Bucket Rotor Loading the Buckets CAUTION If bucket covers are not used, make sure the superstructure of the blood bag protruding from the cup does not inhibit the bucket from reaching its horizontal position. If it does, remove the cup from the rotor and reposition the blood bag so that it seats further into the cup.

  • Page 25: Loading Buckets Onto The Yoke

    If only two filled multiwell-plate carriers are run, install them opposite each other in the rotor and run two additional carriers or buckets (they can be empty) to prevent rotor imbalance. Insert the filled plate(s) into the carriage. If using two or three Beckman Coulter plates per carrier, place a 96-well cap strip (see • Table 3) between the plates to prevent breakage during centrifugation.

  • Page 26: Operation

    Refer to the instrument instruction manual for centrifuge operation. NOTE When using multiwell-plate carriers, enter SX4750A as the rotor and limit top speed of the instrument to 3750 RPM in an Allegra X-12 series centrifuge, 4000 RPM in an Allegra X-14 series centrifuge, or 4450 RPM in an Allegra X-15R centrifuge (when running up to three stacked Beckman Coulter plates separated by cap strips;...

  • Page 27: Removal And Sample Recovery

    Coulter multiwell plates are shown in Table Temperature Limits • Beckman Coulter plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and 25°C. For centrifugation at other temperatures, pretest tubes under anticipated run conditions.
  • Page 28 SX4750A Swinging-Bucket Rotor Operation Table 1 Adapters and Blood Bag Cups Available for the SX4750A Rotor MODULAR DISK ADAPTERS (polypropylene) Color Nom. Nom. Max. No. Max. No. RCF at Adapter Part No. Code Tube Tube Tubes per Tubes in Maximum...
  • Page 29 SX4750A Swinging-Bucket Rotor Operation CELL CULTURE FLASK ADAPTERS (EPDM) Color Code ) Number Flasks at Flask RCF at Max mum Part Number (qty Flask Size (cm per Adapter Speed ( g) two) Bottom (mm) orange 195.3 2080 369292 green 177.5...

  • Page 30: Beckman Coulter Tubes And Bottles For The Sx4750A Rotor

    Tube racks used with Aerosolve cannisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other manufacturers’...
  • Page 31 SX4750A Swinging-Bucket Rotor Operation Table 2 Beckman Coulter Tubes and Bottles for the SX4750A Rotor (Continued) OPEN-TOP TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 18 98 mm polypropylene 342082 359473 359152 29  104 mm...
  • Page 32 SX4750A Swinging-Bucket Rotor Operation b. Adapter used in Aerosolve cannister (part number 359232). c. Tube retainers and adapter plates are sold individually. CONICAL TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 62  141 mm...

  • Page 33: Beckman Coulter Labware For Use With Multiwell-Plate Carriers

    These tubes cannot be run with bucket covers in place. d. Replacement bottle cap part number is 356263 (set of 6). e. Do not load the 750-mL bottle directly into the bucket; always use the adapter. Table 3 Beckman Coulter Labware for Use with Multiwell-Plate Carriers Description Volume...

  • Page 34: Run Speeds

    SX4750A Swinging-Bucket Rotor Operation a. Caps are optional; however, if stacking two or three multiwell plates, use cap strips between plates for speeds greater than 2700 RPM. b. Requires soft rubber roller (4-in.), part number 538618, for installation. Table 4 Commercially Available Labware for Use with Multiwell-Plate Carriers...

  • Page 35: Care And Maintenance

    SX4750A Swinging-Bucket Rotor Care and Maintenance (Up to three Beckman Coulter plates can be run per carrier at maximum multiwell-plate speeds. If running four Beckman Coulter plates, reduce run speed to 2700 RPM. Observe manufacturer’s run speed and stacking limitations if using other manufacturers’ multiwell plates.) If using multiwell plates for runs exceeding 2 hours in a warm and/or humid environment, speed reduction may be required to maintain low sample temperatures.

  • Page 36: Cleaning

    SX4750A Swinging-Bucket Rotor Care and Maintenance Figure 4 Lubricating Pivot Pin/Bucket Contact Areas 1. Lubrication Area (both pin sockets) 2. Rotor Pin Cleaning Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred.

  • Page 37: Modular Disk Adapters

    If aluminum rotor components become contaminated with radioactive material, decontaminate them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or †...

  • Page 38: Sterilization And Disinfection

    SX4750A Swinging-Bucket Rotor Care and Maintenance While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.

  • Page 39: Aerosolve Cannisters

    SX4750A Swinging-Bucket Rotor Care and Maintenance Aerosolve Cannisters WARNING When working with potentially hazardous materials, open cannisters in an appropriate hood or biological safety cabinet in case of tube breakage. If a glass tube breaks in an Aerosolve cannister tube rack, discard and replace the O-ring and rubber tube cushion in the base of the rack.

  • Page 40: Storage

    Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. This form may be obtained from your local Beckman Coulter sales office. The form, entitled Returned Material Authorization (RMA) for United States returns or Returned Goods Authorization (RGA) for international returns, should contain the following information: •...

  • Page 41: Supply List

    NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com or by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. Call Beckman Coulter Sales (1-800-742-2345 in the United States) for detailed information on ordering parts and supplies.
  • Page 42 SX4750A Swinging-Bucket Rotor Supply List Neoprene tube cushions for blue rack (pkg of 24) 344117 for orange rack (pkg of 18) 344118 for purple rack (pkg of 18; 12 large, 6 small) 344119 for dark green rack (pkg of 18; 12 large, 6 small) 344120 for yellow rack (pkg of 8;...

  • Page 43: Beckman Coulter, Inc

    Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery...
  • Page 44 Beckman Coulter, Inc. SX4750A Warranty Warranty-2 GX-TB-004EE...
  • Page 46 Centrifugation Products (IN-175) Use and Care of Tubes and Bottles (IN-192) • Using Adapters for Corning 25-cm Cell • Culture Flask in SX4750 and SX4750A Rotors (GX-TB-007) Using Adapters for Corning 75-cm Cell • Culture Flask in SX4750 and SX4750A Rotors...

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