BIO RAD SmartSpec 3000 Instruction Manual page 48

λ λ
This assay allows you to collect absorbance data from up to three different wavelengths
simultaneously. No concentrations are calculated. When you press λ λ , SmartSpec 3000 will
prompt you first for the number of wavelengths to read.
Enter number (1–3) of
wavelengths to read:
• Use the number keys to input the number of wavelengths to be read and press Enter.
SmartSpec 3000 will then ask for each individual wavelength.
Enter wavelength
• Use the number keys to input the wavelength and press Enter.
After all wavelengths are entered, choose if you want to subtract a background reading
from each wavelength. (The same background measurement is subtracted from all
readings.)
Do you want to subtract
a background reading? No
• If you do not want to subtract a background reading, press Enter and SmartSpec
3000 will be ready to collect absorbance data. If you want to subtract a background
reading, press Select to toggle from No to Yes and press Enter.
Enter wavelength for the
background reading:
• Use the number keys to input the wavelength and press Enter.
SmartSpec 3000 is now ready to collect absorbance data at the specified wavelengths. For
best results, you should first zero the SmartSpec 3000 at specified wavelengths by
inserting a blank solution and pressing Read Blank.
Ready to read absorbance
<=Exit Assay
3
#1:
nm
nm
>=Options
45
.