Quick Guide; Section 6 Quick Guide - BIO RAD SmartSpec 3000 Instruction Manual

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Paper Feed
Alpha
Number pad
Section 6

Quick Guide

1. Power up the instrument. It will go through system status check and alert you if there are
problems.
2. Press one of the Assay buttons.
A. DNA/RNA.
i. Choose type of nucleic acid
a. dsDNA, ssDNA or RNA: accept or modify the conversion factor
b. DNA oligo or RNA oligo: input molar extinction coefficient and molecular
ii. Choose whether to subtract background and, if so, specify background
wavelength.
B. Protein
i. Choose type of assay.
a. Bradford. Measure absorbance at 595 nm.
b. Lowry. Measure absorbance at 750 nm.
c. BCA. Measure absorbance at 562 nm.
d. UV. Measure absorbance at 260, 280, and 320 nm.
e. Other. User specifies the wavelength to read.
Cancels the last entry or causes an exit from Setup.
When editing sequence information, press Cancel to
delete a base. When Options are displayed, press Cancel
to return to the assay.
Used to access other menus or screens. Also used to
move the cursor.
Feeds paper through the printer.
The Alpha key allows the user to toggle between alpha
and numeric input for naming stored standard curves or
naming operators. When in alpha mode, an LED is
illuminated on the key. To type an alphabetic character
when in the alpha mode, press one of the number keys
in the left or center columns. To type an A, press the 1
key once; to type a B, press the 1 key twice, and to type
a C, press the 1 key three times. These keys also enable
the user to enter sequence data. Sequence data are input
using the last column of keys on the number pad: to
type an A, press the 3 key; to type a C, press the 6 key;
to type a G, press the 9 key and to type a T or U, press
the exp key.
Used to input numeric values for SmartSpec 3000
calculations. The exp key is used for making entries in
exponential notation, and when it is pressed, an ε ε is
displayed by SmartSpec 3000. It refers to the base 10
and not to the base of the natural logarithm.
weight; otherwise choose method for SmartSpec 3000 to estimate these values.
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