BIO RAD SmartSpec 3000 Instruction Manual page 25

Spectrophotometer
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If you want to download the data, make sure the connections are made to the
computer and then press Enter. If you do not want to download the data, press
Cancel.
Ready to Read Absorbance
<=Exit Assay
After the data are downloaded SmartSpec 3000 will reset for the next kinetic data col-
lection. From the Ready screen, you can change any of the parameters for the kinetic data
collection.
E. OD600. Immediately after the absorbance data are collected, they are displayed
along with the cellular concentration.
OD600 = x.xxx
Conc = xxxe
You can read a second sample by placing the cuvette in the chamber and pressing
Read Sample. You can also return to the Ready screen by pressing Enter.
Ready to Read Absorbance
<=Exit Assay
Available options are Change next sample number and Change number of sample
replicates.
F. λ λ . Immediately after data are collected they are displayed
A480=x.xxx
A680=x.xxx
You can read a second sample by placing the cuvette in the chamber and pressing
Read Sample. You can also return to the Ready screen by pressing Enter.
Ready to Read Absorbance
<=Exit Assay
Available options are Change next sample number and Change number of sample
replicates.
7. Print the data. You can print data as they are collected, but it is more efficient to print
absorbance and concentration data at the end of an assay. For assays other than Scan and
Kinetics, if you press Print, the display will show
1.Print unprinted Samp #s
2.Select # 3. Full Report
Press 3 to print the full report which includes the absorbances and concentrations (if
available) for each sample in the current assay.
Press 1 to print all data collected since the last time you printed.
press 2 to print only one sample
Enter Sample # ______ to
print sample report.
>=Options
xxx
cell/ml
>=Options
A580=x.xxx
Samp #1
>=Options
22

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