BIO RAD SmartSpec 3000 Instruction Manual page 46

Kinetics.
When you press Kinetic, you begin by specifying the reading wavelength.
Enter reading wavelength
for kinetic assay:
• Use the number keys to input the wavelength to be read and press Enter.
Enter duration of timed
assay :
• Use the number keys to input the duration of the assay in seconds and press Enter.
You may collect data for as long as 9999 seconds.
Enter interval of data
collection:
• Use the number keys to input the interval between successive readings and press
Enter. The shortest interval is 1 second and the longest is 999 seconds.
Do you want to subtract
a background reading? No
• If you do not want to subtract a background reading, press Enter and SmartSpec
3000 will be ready to begin collecting kinetic data. If you want to subtract a
background reading, press Select to toggle from No to Yes and press Enter.
Enter wavelength for the
background reading:
• Use the number keys to input the background wavelength and press Enter.
SmartSpec 3000 is now ready to collect kinetic absorbance data at the specified
wavelength. For best results, you should first zero the SmartSpec 3000 by inserting a
cuvette containing blank solution and pressing Read Blank.
Ready to read absorbance
<=Exit Assay
After the kinetic data are collected, SmartSpec 3000 will reset for the next scan and then
display the Ready screen. If you want to change any of the kinetic parameters, you can do
that without exiting the assay if you choose Options from the Ready screen.
nm
seconds
sec
nm
>=Options
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