BIO RAD SmartSpec 3000 Instruction Manual page 37

After all replicates of standard #1 are measured, SmartSpec 3000 asks for the concentration
of standard #2 and directs you to put the replicates into SmartSpec 3000 one at a time until
all are read.
Enter concentration of
Standard #2:
Insert Rep #1 of Std #2
and PRESS Read Sample
Insert Rep #9 of Std #1
and PRESS Read Sample
This process continues until all replicates of all standards have been read.
When collecting data for construction of the standard curve, SmartSpec 3000 does not
display the absorbance data, but you may view by pressing Abs. If you press Abs, you must
indicate if you want the reading of the current replicate or if you want the mean and standard
deviation of the current replicate group. All standard absorbance data can also be printed at
the end of the assay.
<=Show last replicate
>=Show mean and std dev
• Press the left arrow key to view the absorbance of the last replicate.
• Press the right arrow key to view the mean and standard deviation of the absorbance of
current replicate group.
Next SmartSpec 3000 will prompt for the units of concentration. Your standards must
all have the same units. You will only be prompted for units after the first standard.
Std #1 Rep #2
A750
Std #1 Mean
• Press the right arrow key to return to the assay.
After the last replicate of the last standard is measured, SmartSpec 3000 will construct the
standard curve. Skip ahead to C.
__________
•
•
•
or
A750 = x.xxx
>=Continue
or
= x.xxx +- x.xxx
>=Continue
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