BIO RAD SmartSpec 3000 Instruction Manual page 36

• If you have varying numbers of replicates for the different standards, press Select
to toggle from Yes to No and press Enter. Skip ahead to b. If you have the same
number of replicates for each standard, press Enter.
a. If there are the same number of replicates for each standard. You can
have as many a nine replicates for each standard
standard replicates:
• Use the number keys to input the number of standard replicates and press
Enter.
Now SmartSpec 3000 will prompt for the concentration of the first standard and then for
units of concentration.
standard #1:
• Use the number keys to input the concentration of standard #1 and press Enter.
You may enter concentrations with as many as eight significant digits, but all results are
reported with four significant digits because SmartSpec 3000 absorbance data are measured
to four significant digits.
Once you have selected the units of concentration, SmartSpec 3000 will begin to collect
absorbance measurements on the replicates of standard #1. After all replicates of standard #1 are
read, then you will be asked for the concentration of standard #2, and then directed to input the
replicates of standard #2. SmartSpec 3000 will continue in this manner until all replicates of all
standards are measured.
• Insert the cuvette with the first replicate of standard #1 into SmartSpec 3000 and press
Read Sample.
SmartSpec 3000 will continue to prompt for each additional replicate of standard #1 until
all are read.
Insert Rep #2 of Std #1
Insert Rep #9 of Std #2
Enter the number of
Enter concentration of
_________
Insert Rep 1 of Std #1
and PRESS Read Sample
and PRESS Read Sample
•
•
•
and PRESS Read Sample
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