BIO RAD SmartSpec 3000 Instruction Manual page 23

• Press A260:A280 to see the ratio displayed
A260=
A260:A280
• For DNA oligos and RNA oligos, press Conc to see the concentration displayed in
units of pmoles/µl.
A260=
Conc=
• To read the next sample, insert the cuvette in the chamber and press Read Sample.
From any of the above screens you can press Enter to return to the Ready screen.
Ready to Read absorbance
<=Exit Assay
B. Protein. Immediately after the sample is read, the data are displayed in the form
A595=
Conc=
• To read the next sample, insert the cuvette in the chamber and press Read Sample.
From any of the above screens you can press Enter to return to the Ready screen.
Ready to Read Absorbance
<=Exit Assay
The available options from the Ready screen are Change next sample number and Change
number of sample replicates.
C. Scan. Immediately after the absorbances are calculated the screen will display the
message
Press <Print> when ready
<=nm scale
• Press Print to begin printing the scan. If you do not want to print the scan, press
Cancel. From this screen, you can press the left arrow key to select nm scale and
direct SmartSpec 3000 to print only a subset of the data collected.
Begin scanning at ____ nm
End scanning at ____ nm
You can also change the minimum and maximum values on the AU scale, by pressing the
right arrow key.
Min AU scale:
Max AU scale:
• Enter the lower limit. If you want the lower limit to be a negative value, enter the
absolute value of the lower limit and press Enter and then press Select. For example,
if you want the lower limit to be -0.500, press the decimal key and the 5 key and then
Enter. The display will show
Min AU scale: +/-
Max AU scale:
x.xxx A280= x.xxx
= x.xxxx
x.xxx Samp #1
xxx.xxx pmoles/µl
>=Options
x.xxx Samp #1
xxx.xxx µg/ml
>=Options
>=AU scale
0.500
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