BIO RAD SmartSpec 3000 Instruction Manual page 38

b. If there are varying numbers of standard replicates. Each time SmartSpec 3000
prepares to read a standard, you will be prompted for the number of replicates for that
standard and then for the concentration of the standard. After all replicates of the first
standard are read, then SmartSpec 3000 will prompt for the number of replicates of the
second standard and then for the concentration of the second standard. This will continue
until the last replicate of the last standard is read.
Enter number replicates
for Standard #1 (1–9): __
• Use the number keys to input the number of replicates of standard #1 and press Enter.
Now SmartSpec 3000 will prompt for the concentration of the first standard and then for
units of concentration.
Enter concentration of
Standard #1:
• Use the number keys to input the concentration of standard #1 and press Enter.
You may enter concentrations with as many as eight significant digits, but all results are
reported with four significant digits because SmartSpec 3000 absorbance data are
measured to four significant digits.
SmartSpec 3000 will begin to collect absorbance measurements on the replicates of
standard #1.
Insert Rep #1 of Std #1
and press Read Sample
• Insert the cuvette with the first replicate of standard #1 into SmartSpec 3000 and
press Read Sample.
SmartSpec 3000 will continue to prompt for each additional replicate of standard #1 until
all are read.
Insert Rep #2 of Std #1
and press Read Sample
Insert Rep #9 of Std #1
and press Read Sample
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