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•
Use the number keys to input the length of the oligo and press Enter. You can
enter a length of up to 99 nucleotides. After you press Enter, SmartSpec 3000 will
calculate and display the extinction coefficient and molecular weight.
Mol ext coef=
MW= __________ >=Continue
The molar extinction coefficient is displayed with units of liter mole
•
Press the right arrow to continue the assay. SmartSpec 3000 will now be ready
to measure the absorbance of your oligonucleotide and to convert those
absorbance data into concentrations of µg/ml and pmol/µl.
Ready to read absorbance
<=Exit Assay
b. Composition.
Enter oligo composition:
#A:___#C:___#G:___#T:___
•
Use the number keys to input the number of each type of nucleotide. Use the
arrow keys to move from field to field. Press Enter when all four have been
specified. SmartSpec 3000 will calculate and display the extinction coefficient
and the molecular weight.
Mol ext coef= __________
MW= __________ >=Continue
The molar extinction coefficient is displayed with units of liter mole
•
Press the right arrow to continue the assay. SmartSpec 3000 will now be ready
to measure the absorbance of your oligonucleotide and to convert those
absorbance data into concentrations of µg/ml and pmol/µl.
Ready to read absorbance
<=Exit Assay
c. Sequence
Enter oligo sequence:
_______________________
•
SmartSpec 3000 will automatically shift to the Alpha mode on the keypad so
that you enter an A by pressing the 3 key, a C by pressing the 6 key, a G by pressing
the 9 key and a T or U by pressing the exp key. You may enter a sequence of up
to 60 nucleotides. As you enter sequence, it will scroll to the left. You can move
around in the sequence with the arrow keys. Put the cursor under a nucleotide
and press Cancel to delete it. Put the cursor under a nucleotide in front of which
you want to make an insertion, then type the base to be inserted. Press Enter
when the sequence has been completely specified. SmartSpec 3000 will calculate
and display the extinction coefficient and molecular weight of the oligo.
Mol ext coef=
MW= __________ >=Continue
The molar extinction coefficient is displayed with units of liter mole
>=Options
>=Options
__________
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