BIO RAD SmartSpec 3000 Instruction Manual page 47

Spectrophotometer
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OD 600.
This assay is useful for monitoring growth of bacterial cultures. When you press OD600,
SmartSpec 3000 will display the current conversion factor and offer you the choice of
accepting or modifying it.
A600 1.0=xx.xexx cell/ml
Is this factor OK? Yes
If the displayed conversion factor is acceptable, press Enter. Otherwise, press Select
to toggle from Yes to No and press Enter.
Enter new conversion
factor;
Use the number keys and the exp key to input the new conversion factor and press
Enter.
SmartSpec 3000 is now ready to collect OD600 readings. For best results, you should first
zero the SmartSpec 3000 by inserting a cuvette with media (without cells) and pressing
Read Blank.
Ready to read absorbance
<=Exit Assay
xx.xexx
cell/ml
>=Options
44

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