Preparing The Detector; Preparing The Hplc System - Agilent Technologies InfinityLab LC Series User Manual

4 Preparing the Module
Setting up an Analysis

Preparing the Detector

For best performance of the detector
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Preparing the HPLC System

1 Turn on the control software and the monitor.
2 Turn on the modules.
3 Start the control software. The screen should show all modules and the
4 Turn on the modules that require conditioning:
5 Load the default method.
6 Pump the water/acetonitrile (30/70 %) mobile phase through the column for
Agilent InfinityLab LC Series VWD User Manual
Let the lamp warm-up and stabilize for at least one hour (initial turn on of the
module requires a longer time depending on the environment and the
application needs); refer to
For high sensitivity measurements, a stable environment is required; refer to
"Environment" on page 28. Prevent drafts from air condition systems.
Do not work with removed/open front panels/doors. When the system
includes a G1316 TCC (typically located below the detector) and its front
panel is removed while the TCC is set to high temperatures, the up-streaming
air could influence the stability of the detector baseline.
system status is Not Ready.
a Detector lamp (warm-up for at least 60 min to get a stable baseline).
b Column compartment (set temperature as required).
c Pump (purge).
d Sampler (prepare the standard isocratic sample into a vial).
e Solvents (fill water and Acetontrile into the solvent bottles).
10 min for equilibration.
"Specification Conditions" on page 34.
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