Agilent Technologies InfinityLab LC Series User Manual page 58

4 Preparing the Module
Setting up an Analysis
Table 7 Choice of Priming Solvents for Different Purposes
Activity
After an installation
When switching between reverse
phase and normal phase (both times)
After an installation
To clean the system when using buf-
fers
After a solvent change
After the installation of normal phase
seals (P/N 0905-1420)
The pump should never be used for priming empty tubings (never let the pump
NOTE
run dry). Use a syringe to draw enough solvent for completely filling the tubings
to the pump inlet before continuing to prime with the pump.
1 Open the purge valve of your pump (by turning it counterclockwise) and set
2 Flush all tubes with at least 30 mL of solvent.
3 Set flow to required value of your application and close the purge valve.
Pump for approximately 10minutes before starting your application.
NOTE
Agilent InfinityLab LC Series VWD User Manual
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Hexane + 5% Isopropanol
flow rate to 3 – 5 mL/min.
Comments
Best solvent to flush air out of the sys-
tem
Best solvent to flush air out of the sys-
tem
Alternative to Isopropanol (second
choice) if no Isopropanol is available
Best solvent to re-dissolve buffer crys-
tals
Best solvent to re-dissolve buffer crys-
tals
Good wetting properties
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