Agilent Technologies InfinityLab LC Series User Manual page 73

5 Optimizing the Detector
Match the Flow Cell to the Column
Figure 21
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the detector, it is necessary to have more precise information on
path lengths of the flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the flow cells:
Table 10 Correction factors for Agilent VWD flow cells
Part number
Standard flow cell 10 mm, 14 µL, 40 bar (G1314-60186)
Semi-micro flow cell 6 mm, 5 µL (G1314-60183)
Micro flow cell 3 mm, 2 µL, 120 bar (G1314-60187)
High pressure flow cell 10 mm, 14 µL, 400 bar (G1314-60182)
However you have to be aware that there is additional tolerance of gasket
NOTE
thickness and its compression ratio which is supposed to be very small in
comparison with the machining tolerance.
Agilent InfinityLab LC Series VWD User Manual
Time (min)
Influence of Cell Path Length on Signal Height
Analysis of pesticide standard
6-mm optical path length
10-mm optical path length
Path length (actual)
10.15 ± 0.19 mm
6.10 ± 0.19 mm
2.80 ± 0.19 mm
10.00 ± 0.19 mm
Correction factor
10/10.15
6/6.10
3/2.8
10/10
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