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Standard
protein
solution
BSA
-
I mg
Double distilled water
-
I ml
Sodium dodecyl sulphate-polyacrylanlide gel electrophoresis
(SDS-PAGE:)
materials
Acrylarnide stock (30%)
Acrylamide
-
29.2
g
Methyl bisacrylamide
-
0 . 8 g
Double distilled water
-
100ml
The acrylamide solution was deionized with Amberlire (I d l 0 0 ml), tiltcrcd through
Whatman No.1 paper and stored in brown hottle at 4
"C.
Separating gel
(5
mi)
10%
Double distilled water
-
1.9 ml
Acrylamide : Bisacrylamide (30%)
-
1.7 ml
1.5 M Tris
HCl
(pH 8.8)
-
1.3ml
10% SDS
-
0.05 ml
10% Ammonium persulphate
-
0.05 ml
TEMED
-
0.002 ml
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