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Fig.
14 SDS-PAGE
analysis of recombinant
1,TP
fusion protein.
Lanc M. I'rotein
111i1lecular weight marker
gene^.
Bangalore, India): Lane I . total ccll protein from
uninduced E. coli BL-21 (DE.7)
containing
pMAL-LTP. L.anc 2, total cell protein
from induced
E.
roli
BL-21 (DE3) conta~ning pMAL-LTP: Lane 3, pellcl frcrm
ccntnfugation of induced son~cated cella; Lane 4, rupernatant I'rom centrifugauon of
Induced sonicated cells: Lane 5, purified LTP-MBP I'ur~on protein. The arrow
Indicates the MBP-LTP fusion protein (51 kDa).
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