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Hindlll
Barn HI
Barn HI
Hind ill
EcoRl
Hind IN
5'
3'
5
'
3'
5'
3'
Ubiprornoter
Gene
(Ltp
3F
1
/BwZcM)
Cah+mir
I
Ligation
I
Hind Ill
Barn HI
Barn HI
Hindltl
5'
3'
5'
3'
J
Hind Ill
I
ClAP
Hind Ill
Barn HI
Three-Fragment Ligation
+
Hlnd Ill
H
i
n
d
I L : ,
EcoRl
1
EcoRl
pcmnein -w
PCAMBIA -cahL@ 3F 1 / Bw2chi
Fig. 28 Construction of gene cassettes for plant transformation. Three-fragment
ligation
was
performed using pCAMBlA 1300 to construct the gene cassettes.
pCAMBIA-cah-Ltp
3FI and
pCAMBI.4-cah-BarZchi.
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