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DNA loading
dye
Glycerol
EDTA (pH 8.0)
Bromophenol blue
Protein estimation reagents
Alkaline copper reagent
Solution A (2% sodium carbonate in 0.1 N NaOH)
N ~ z C O J
-
I g
0.
I
N NaOH
-
50 ml
Solution
B (1%
C S 0 4 )
c u s o 4
-
0 . 1 g
Double distilled water
-
lOm1
Solution
C (2%
sodium potassium tartarate)
Sodium potassium tawrate
-
0.2 g
Double distilled water
-
lOml
Prior to use 49 ml of solution A was mixed with each 0.5 ml of solution
B
and C
Folin Ciocalteau reagent
The commercially available Fohn reagent was diluted with equal volume of water. or
in the dilution ratio as specified by the manufacturer (SD Fine-Chern. Ltd.. Murnbai).
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