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Fig. 3 3
PCR
analysis of transgenic tobacco plants containing pCAMBIA-cah-Up
3F1. ( A ) Detection of Lrp gene using gene-rpec~iic primers (348 hp). Lane M, I kh
DNA ladder; Lane 1-8, transformants showing amplification of the desired s~zed gene
fragment$. Lane
9,
untransfbrmed control (negative control) and Lane 10, plasmid
pCAMBIA-cuh-Lrp 3F1 (positive control). (B) Southern blot analysis of 1 . 1 1 , in
transformants. Lane C, positive control, pCAMB1A-cuh-Lfp 3FI. Lanes 1-8,
transgenic plants, plant 2 (lane 2) and plant
4
(lane
4)
showing one hybridizahle band
and other 6 transformants, plant 1, 3.
5-8
(lanes
I .
3 . 5-8) showing two hybr~dizable
hands for
Lrp
gene.
Lane
9, untransformed control plant
(C)
Western blot showed
expression of wheat
LTP 9 kDa
polypeptide in all the 8 transformants. where& such
expression was absent in the untransformed control plant.
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