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I \TG TCT TCT TCA CAA GTC AAA GCC AAC GCA TGG ACT GCC GTT CCA GTC ACC (;CA AAG CCC
( \ I S
S
S
E
l
4
\
T
4
P
\
4
4
h
\
61
4TT
GTT GAC TCC CTC GGA
GGT GAT GTC TCC TCA TAT T ( T CTG GAA GAT ATC (;CG
! , I \
D
S
I
.
(
;
b
L
(
;
U
\
S
h
\
S
\
F
I
)
I
h
121 TTC CCT GCG GCA GAC AAA CTT GTT GCC GAG CCA CAG GCC
lTT
GTG AAC GCC CCA TTG ACT
J I I ' E T
H
I
F
I
\
I
4
W K
181 CCC GAA ACC TAC AAT CAC TCC ATC CGC GTT TTC TAT TGC C(;A ACT (;TC ATC (;CG ACA CCT
, 1 1 1
I .
P A 4 l ) h :
I . \
\ t : \
Q
\
F
\
h
\
R
I
.
241
I'TA CTT CCC GAG CAA GCT AAA GAT TTC TCT CCA ACT ACA TGC GCA CTC ACA TGT CTT CTC
n
l
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I.
P
E
Q
\
h I l 1 .
h
P
4
I
\ \ \
I
' I
( ' I , l
1 1 1 1
(.4T CAT GTT CGT ACT GCC GAG GTA T.4C TTT ACA TCT A('A C(;A AT(; TCC TT(' (;AT ATT TAC
1 , 1 1 1 1 1)
\
C
T
h
t
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t ' l
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5
k
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M I I;(;T GCC ATTAAG CCT ATG GAG CTC; CTC AAC GTC CTT
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ACT A(;C A('C(;Ac'CA(; (;('T (;A(;
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a
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121 (;CT GTT GCC GAG CCC ATC ATT CCT CAT CA(; CAT (:TC CC(; (;TA (;AT
(;(;('
AA(' AT('A('A TTl'
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4
I
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I
I
H I 1
b
1)
1 1 ;
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I > ( , \
I
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481 ("I C GCT CAC TTG ATC CAC CTC GCT ACC CTT TAT (;AC AAT (:TC
(;C(;
<;C<'TA(' (;AT G(;(; AI'T
I
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I
0
I
1
'r
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I)
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I
( ;
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541 ( A T CAT TTT GGT AGC TCG GTT CAT CAC ACC AC4 C(;('AA(' ACT ATC AA(' A('(; (;('A TT(' C('A
h
l
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F
(
5
M
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I
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t
1 '
M l l
('(;A CAT GCT TGG TGT TCT TGC TTT CCC TG(' AC(; (;TT CCT AA(; (;AA CAA A(iT AA(' AAG
( ' ( ' I '
2111
K
I I
<;
M
( '
L
H
t
\
('
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W
h
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1,
4
\
h
I'
lhl T(;G TGC CAC ACA AC(; CAT ATC CCT CAC TTC CAT AAA CA(; AT(; (;AA
<;(' < ;
AA(' ACT TTG AT(;
I
\
(
I
1
T
H
I
P
0
t
II
h
0
\ I
t
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I
I
hl
721 \4<; CCT TGG GAG
! I l h
P
M
t
Fig. 23 Nucleotide and amino acid sequence of cyanamide hydratase
(coh)
gene.
I h d e r l i n e d sequence indicates the H b ~ d l l l site. In order to eliminate the H i n d l l l 1 1 e .
In
cah
M u t
K
mutant [he single base substitution,
G
L o
A
and
in c a h Mul
L
mutant [he
w g l e base substitution,
T
t o
C
w a 5
generated without the change of a m m o acld
q u e n c e .
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