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4.3. Amplification of chitinase and lipid transfer protein genes
When the barley cDNA clone
(Bur2chi)
was used as template, the gene-
.pecific primers successfully amplified chitinase gene of 1.1 kh size
(Fig.
7). The
nucleotide and amino acid sequence of harley chitinase gene is presented (Fig. R).
When the wheat cDNA clone ( L t p 3FI
)
was used as template. gene-specific primer\
\uccessfully amplified wheal lipid transfer protern gene of' 348 hp size (Fig. 9). The
nucleotide and ammo acid sequence of wheat lipid transfer protein gene is presented
(Fig. 101.
Fig.
' 7
Amplification of DNA corresponding to the sequence encoding barley
chitinase.
Agarose gel: Lane 1, I kb DNA ladder (Promega, Madison, WI USA);
Lane
2, Bur2chi
gene ( I .
1
kb).
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