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Present investigation was aimed for construction of plant transfomntion
cassettes and heterologous expression of cyanamide hydratase (cah) marker gene and
antifungal genes encoding chitinase and lipid transfer protein (LTP).
Objectives of the present study have been formulated as follows:
I.
Cloning and expression of chitinase and LTP genes in
E. coli
and evaluation
of their antimicrobial potential towards phytopathogens.
2.
Site-directed mutagenesis, cloning and expression of cuh gene in
E. coli
3. Construction of gene cassettes with cah marker and antifungal gents encoding
chitinase and LTP.
4. Transgenic expression of cah marker and antifungal genes in tobacco
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