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Murashige and
Skoog
(MS) medium (Murashige and Skoog, 1962)
Chemical
Final conc.
/
litre
StocW25O ml
Quantity to
be
dispensed
of the medium
for
1
litre MS n~ediuni
MnSOd.H20
22.3 mg
223 mg
ZnS04.7H20
8.6 mg
86.0 mg
H ~ B O J
6.2 mg
62.0 mg
KI
0.83 mg
8.3 mg
Na2Mo04.2H20
0.25 mg
2.5 mg
CuS04.5HzO
0.025 mg
0.25 mg
CoC12.6HzO
0.025 mg
0.25 mg
FeEDTA
40.0 mg
1.0 g
L O ml
Nicotinic acid
0.5 mg
12.5 rng
Pyridoxine
0.5 rng
12.5 rng
10 ml
Thiamine HC1
0.1 rng
2.5 mg
Glycine
2.0 mg
50.0 mg
Myoinositol
100.0 mg
Sucrose
30.0 g
Added as solid
PH
5.8
Adjusted with 0.1 N KOH and 0.1 N HCI
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