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Table 6
List
of plasmids, cDNA clones and antisera used in this study
plasmid,
Relevant characteristics
Source
cDNA clone
and antiserum
pQE-60
Veclor slrc 343 1 hp.
T5
promoter
/ lac
oper;tlor,
ColEl origin of replication, Amp R
-gcnc,
C-terminal6x Hls-tag.
pET 28 a+
Vector size 5369 hp,
77
Promoter.
pBR322 urigin of replicntion. f1 origin,
Kan
R-
gene, N and C-terminal 6x HIS-tag
pMAL-p2x
Vcclor sizc 6721 bp. roc promoter, lac nper;ilor.
M I 3 origin, pBR322 origin
of
rcplicalion,
Amp R -gene, molE gene N-terminal fusion.
pCAM
Vector size 5,727 bp. Ubi I promoter, cah gene.
nos 3' terminator, Amp R -gene, pUC8 backbane.
pCAMBlA 1300
Veclor slze 8958 bp, hprll R-gene planls,
Kan R-gene bacteria, pUC18 polylinker.
T-DNA s i ~ e 2728 bp. MSV veclor family.
Borlchi
cDNA clone from a library of
Erysiphe graminis-infected barley
Llp
3FI
EDNA clone from a library of
Fusorium
grarnineurum-infecled wheat
Antiserum Cah
Cyanamide hydralase antiserum
Antiberum Bar2chi
Barley chitinase antiserum
Antisemm LTP
Wheal lipid transfer protein antiserum
Qiogcn. CA. USA
Novogcn. USA
New Englond
Biolabs Bevcrly. USA
J. Troy Weeks
University of Nehraska. USA.
R. Jefferson. CAMBIA.
Canherra. Australia.
W. Li, and S. Mulhuhshnan
Kansas Stale University, USA
W. Li, and S. Muthukrishnan
Kansas State University. USA.
S. Muthukrishnan
Kansas Stole University. USA.
S. Muthukrishnan
Kansas Slnte University, USA.
Tranquet Olivier, INRA, France
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