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4.11. Antifungal
assays
of purified LTP
The purified LTP fusion protein showed a broad-spectrum antifung:~l :~ctivity
at a concentration of 100 and 300 pg against illrrrri~rrirt sp. (grain ~liscolor:~tion
of
rice), R. soloni (sheath blight of rice) (Fig. 21). C I~ttioro (Icaf spot of clover).
0.
oryzoe (brown spot o f rice). Cylindrocludittrn scopnriurii (root necrosis of h;lnana) ; ~ n d
Sorocludium oryzue (sheath rot of rice). When the mycelium of
C.
irrr~~tru m d
Airemuria sp. from the periphery of the zone of inhibition produced by purified LTP
fusion protein was examined under light microscope ( ~ 4 0 0 ) . the hyphne appeared to
have clear cut mycelial deformations such as poorly developed mycelium with
swollen margin, whereas the mycelium from the control plate was normill, branched.
well-developed and intact (Fig. 22 A and
B).
4.12. Effect of
LTP
on germination of spores
Spore germination assays revealed the fungistatic effects of LTP against
phytopathogenic fungi such as M. griseu,
M.
phuseolinu. Fusurium sp.,
A.
i.inereu,
S.
uryzue and
B.
oryzae. Fungicidal activity was observed against Alrernuriu sp. and C.
lunatu. The control spores showed germination but LTP fusion protein treated spores
did not show germination (Fig. 22 C and
D).
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