Advertisement
4.153.
Cyanamide tolerance leaf dip
assay
When thirty day old transformants were bubjected to cyanamide leaf dip assay
dong with the non-transformed control plants by applying 5% cyilnwlide solulion as
. d a c e spray. Transformed plants alone retained thetr healthy grren appearance.
uhere as leaves o f susceptible untransformed control plant\ scorched within 72 h of
cyanamide treatment. The treated plant\ grew to maturity and set heeds
U I ~ ~ O U I
any
~pparent side effects due to cyanamide treatment. T h e e results ~ n d ~ c i ~ t e d
the fact that
cyanamide can be employed as a solution or in granulc l'nrrn f'or effcct~vc mean* of
~drntifying transgenic plants from the non-transgenic plants In
a
populat~on of
\egregating progeny plants (Fig. 30).
Fig. 30 Cyanamide tolerance assay. Cyanamide tolerance depicted after foliar
application of 5% cyanamide.
( A ) Untransformed control and (8) transformed plant
after treatment.
Advertisement
