Mitsubishi Electric MXZ-18TV -E1 Service Manual page 59

2.1.3.
Antibiotics, chemicals and their stock solutions
Antibiotics, chemicals and their working concentrations are presented in Tr~bie 8. The
stock solutions were made tiltcr sterilized using 0.22 pm filters (Millipore. Molsheim.
France) stored at -20
'C.
3.1.4. Restriction endonuclense enzymes
All restriction endonucleases and
T4
DNA iigase were purchased from
Promega, Madison, WI, USA and other molecular biology buffers and reagents were
obtained from Bangalore Genei, Bangalore. India.
Table 8 List of antibiotics, chemicals a n d their concentrations used in this study
Antibioticsf Stock solution Working concentration
Chemicals ( m d m l ) (pdml)
Ampicillin
100
(in water)
Kanamycin 100 (in water)
X-gal 20
(in DMSO)
lPTG 23.8 (in water)
Ethidium bromide 10 (in water)