Multi-Wavelength Mode - Measurements At More Than One Wavelength; Set The Reading Mode At Different Wavelengths - Hach DR 5000 User Manual

Spectrophotometer
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Advanced Operations
6.5 Multi-Wavelength mode – measurements at more than one wavelength

6.5.1 Set the reading mode at different wavelengths

Options
More
Store Off/On
% Trans/Abs
λ
Wavelength
Timer icon
Concentration Factor
Concentration
Resolution
Absorbance Formula
Save as User Program
90
In the multi-wavelength mode, absorbance values can be
measured at up to four wavelengths and the results can be
mathematically processed to obtain sums, differences and
relationships.
Absorbance measures the amount of light absorbed by the sample,
in units of absorbance.
% Transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turning on the concentration factor allows selection of a specific
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.
Press Multi Wavelength in the Main Menu. Press Options for
Parameter Setup.
Table 13 Multi-wavelength setup options
Description
For further Options
With the Store On setting, all measurement data are stored automatically. With the Store Off
setting, no measurement data are stored.
To switch to % transmittance, concentration or absorbance readings
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement
wavelength. The entered wavelength must be in the range from 190–1100 nm.
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
Multiplication factor for converting absorbance values into concentration values.
To select the position of the decimal point in the calculated concentration readings.
Calculation basis for evaluating samples
To store the selected parameters as a User Program, see
section 6.1 on page 61

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