Single Wavelength (Absorbance, Concentration And Transmittance Measurements); Set Up Single Wavelength Mode - Hach DR 5000 User Manual

Spectrophotometer
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6.4 Single Wavelength (absorbance, concentration and transmittance
measurements)

6.4.1 Set up single wavelength mode

Options
More
Store Off/On
% Trans/Abs
λ
Wavelength
Timer icon
Concentration Factor
Concentration
Resolution
The Single Wavelength mode can be used in three ways. For
sample measurements at a single wavelength, the instrument can
be programmed to measure the absorbance, % transmittance or
concentration of the analyte.
Absorbance measures the amount of light absorbed by the sample,
in units of absorbance.
% transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turn the concentration factor on to select a specific multiplier for
converting absorbance readings to concentration. In a graph of
concentration versus the absorbance, the concentration factor is
the slope of the line.
Press Single Wavelength in the Main Menu. Press Options for
Parameter Setup.
Table 12 Single wavelength setup options
Description
For further Options
With the Store On setting, all measurement data are stored automatically. With the Store Off
setting, no measurement data are stored.
To switch to % transmittance, concentration or absorbance readings
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement
wavelength. The entered wavelength must be in the range from 190–1100 nm.
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
Multiplication factor for converting absorbance values into concentration values.
To select the position of the decimal point in the calculated concentration readings.
Advanced Operations
87

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