Sterilising The Dish Inserts; Validation Check; Gas Calibration - K-Systems G210 InviCell User Manual

Section 12
Maintenance

Sterilising the Dish Inserts

Use this procedure in cases of contamination and/or spillage:
Packaging:
1. Promptly soak up excess liquid using a sterile cloth.
2. Remove the Dish Insert from the incubator chamber.
3. Moisten a sterile cloth with sterile water and wipe all surfaces, especially all of the milled
grooves.
4. Wrap Dish Inserts in a breathable sterilisation wrap or place in a sterilisation pouch
in accordance with local procedures. In the United States (US), use an FDA-cleared
sterilisation wrap or sterilisation pouch.
Steam Sterilisation:
5. Use a validated, properly maintained and calibrated steam steriliser.
6. Effective steam sterilisation can be achieved using the following cycle:
Cycle Type
Gravity 121°C (250°F)
For further guidance for Sterilisation, refer to: ANSI/AAMI ST79 "Comprehensive guide to
steam sterilisation and sterility assurance in health care facilities."

Validation Check

Perform the following validation gas and temperature checks after all cleaning, disinfecting
and sterilising or at least every two weeks to ensure the G210 InviCell is operating correctly.

Gas Calibration

It is very important that the G210 is not emptied of gas during the gas calibration procedure.
To do so will cause unstable gas levels and gas flow and will result in a considerable time
before the gas concentration is recovered and becomes reliable and stable again.
To ensure the G210 is not emptied of gas during the gas sampling please follow the below
instructions.
There are two methods for collecting gas samples from the incubator.
1. Via the Gas Sample Port (located behind the Preparation Chamber, and connected
directly to the internal gas mixing chamber)
2. Via the silicone plug on each of the chamber lids
12 Always check the gas concentration in the setpoint menu during sampling. If the gas
concentration differs more than 0.1% from the setpoint, allow the G210 to stabilise to the
gas concentration set point before taking the next gas sample.
Allow 2 minutes between samples to allow the incubator to stabilise.
Please Note: To ensure accurate and reliable gas measurements, please use a high-quality
calibrated gas analyser. The calibration procedure described here assumes the use of the
G100 gas analyser (Order Code: 11103).
44
Minimum Temperature Minimum Exposure Time / Dry Time
30 minutes / 30 minutes dry time