Nikon ECLIPSE E400 POL Instructions Manual page 15

2. Microscopic Procedure
The settings of each part in orthoscopic m
refer to the previous
Orthoscopic Observation
Conoscopic Observation
Orthoscopic Observation/Conoscopic Observation NENNEN
The following provides an explanation of the characteristic observation method of polarizing
microscopes along with the microscopy procedure. If normal microscopy has not yet been completed,
section and complete normal microscopy.
In this method, the specimen is observed with
the polarizer and analyzer placed in the
optical path.
Operation
Pull out the analyzer slider and move the
analyzer into the optical path.
In this case. the shape of the specimen is
visible (direction of optical axis) and the
optical properties relative to the direction of
the thickness of the specimen can be
observed.
Analyzer in the optical path
In this method. in addition to the polarizer
and analyzer, the Bertrand lens is also moved
into the optical path when observing a
specimen.
Operation
Move the analyzer into the optical path. and
place the Bertrand lens turret of the
intermediate tube in position "В" to move the
Bertrand lens into the optical path. (Refer to
p.23 for details regarding the focusing
procedure.) Place the P-CL 1/42. & tint plate
in the hollow position. Use an objective
[B]
Analyzer and Bertrand lens
in the optical path
having a large numerical aperture (high
magnification: normally 40 or higher)
Specimens can be observed from various angles with diascopic light in the form of a single
image. Differing from orthoscopic observation, however, the shape of the specimen itself is
not visible with this observation.
croscopy and conoscopic microscopy are summarized below.
'Orthoscopic Observation Conoscopic Observation
- To investigate the vibration direction and birefringence | To distinguish between uniaxial and biaxial
observation | of observation light by observing the extinction and properties and observe the optic-axial angle
interference color due to the specimen. and optical characteristics in minerals.
Toplensor | 10x or higher IN
the condenser | 4x or lower
OUT
m
Bertrand lens 'OUT (Turret position: O) IN (Turret position: В)
70 - 80% of the erical с Арепше 10x or higher | о ыы ыы | Circumscribed the circumference of the
diaphragm z conoscopic field of view (or fully opened)
4x or lower Fully opened
^ = ibed if Field 10x or higher | Cireumscribed the circumference of | Cireumscribed the circumference of the
the eyepiece field of view
diaphragm orthoscopic field of view
dx or lower Fully opened
13