Nikon ECLIPSE E400 POL Instructions Manual page 26

Centering the objectives
ul Before centering the objectives, focus on a specimen using the 10x objective.
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d Tools Used: Centering tools x 2 (provided) (when not in use, place them in the tool holder
Г ne the back of the microscope stand).
n 1 Bring an appropriate target such as granules that can be easily used as a marker in the
specimen to the center of the crosshairs of the eyepiece.
Insert the centering tools into the centering screws on the nosepiece.
Rotate the stage about 180°. Move the objective using the centering tools so that the cer
of the crosshairs moves by one-half the amount of movement of the target.
Next, move the specimen and bring the target to the center of the crosshairs.
Repeat this procedure several times. Carry out this centering procedure for each objecti
en
an
180° rotation
Middle point
the move
Target
ШІ Circular graduated stage for polarizing microscope
p
Stage rotation
Loosening the stage rotation clamp screw can rotate
the stage. The angle of rotation can be read to 0.1
degrees from the two vernier scales.
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